Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers

Amal Saad-Hussein; Mona Mohamed Taha

doi:10.1515/jcim-2019-0166

Article

Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers

Amal Saad-Hussein and Mona Mohamed Taha

Published/Copyright: December 25, 2020

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From the journal Journal of Complementary and Integrative Medicine Volume 17 Issue 4

Abstract

Background

Epigenetic represents a study of occurred heritable gene expression changes without changing in the DNA sequence. It includes DNA methylation and miRNA expression that attract increasing attention as potential links between the genetic and environmental determinants of health and disease. Pesticide exposure is associated with adverse health effects and DNA methylation due to oxidative stress induced following its exposure. This study aimed to define the association of genetic polymorphisms of XRCC1, PON1, GSTP1 and GST genes with global genes DNA methylation in urban and rural occupationally pesticides exposed workers.

Methods

This study included 100 pesticides exposed workers; 50 rural sprayers (RE) and 50 urban researchers (UE). Controls included equal numbers. DNA methylation of global genes was evaluated by pyrosequencing assay. XRCC1, PON1 and GSTP1 genotyping were assessed by PCR–RFLP, and GST M1 and T1 were performed by PCR.

Results

The results of this study revealed that most genotypes in XRCC1, PON1, GSTP1 and GST genes were associated with LINE-1 hypomethylation among UE group. However, heterozygote genotypes (Gln-Arg and Ile-Val) in XRCC1 and GSTP1 genes, respectively, were associated with LINE-1 hypermethylation among UE compared with other corresponding genotypes. Only GSTT1 polymorphism recorded a significant change in percent methylation of Alu elements among urban and rural groups.

Conclusion

Urbanization could play an additional risk for epigenetic changes associated with pesticide exposure, and that could be attributed to the quality of life including their dietary habits, working and living in closed areas, and their exposure to extra pollutions emitted from urbanization sources.

Keywords: DNA methylation; gene polymorphism; GST; pesticides exposed workers; PON1; XRCC1

Acknowledgments

We are grateful to the laboratory and field staff of the “Wide range Medical Molecular Laboratory Research Unit, Digital Molecular Biology Research Lab” for their contributions. We are especially thankful to Prof. Dr Hanaa Mohamed Rasmy for their help through using the PSQ Q96 MD Pyrosequencing system (QIAGEN, Valencia, CA) in this study.

Author contributions: Dr Amal Saad-Hussein designed the study and performed statistical analyses and tabulated the results. Dr Mona M. Taha performed laboratory analysis and drafted the manuscript. All authors revised the final manuscript and approved their responsibility for the work.
Research funding: This study was supported by grants from the Science and Technology development Fund (STDF), Egypt.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The authors declared there is no conflict of interest in this work content.

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Received: 2019-06-25

Accepted: 2019-11-11

Published Online: 2020-12-25

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Articles in the same Issue

https://doi.org/10.1515/jcim-2019-0166

Keywords for this article

DNA methylation; gene polymorphism; GST; pesticides exposed workers; PON1; XRCC1