Modulatory effect of Polyalthia longifolia leaves against cadmium-induced oxidative stress and hepatotoxicity in rats
Abstract
Background
Cadmium serves as a major pollutant in the environment and it has been documented for its widespread harmful effects. This study sought to investigate the prophylactic and the curative effects of aqueous and methanolic leaf extracts of Polyalthia longifolia against cadmium-induced hepatotoxicity in rats.
Methods
Animals in group I served as the normal control and administered distilled water only for 14 days, group II was administered cadmium (4 mg/kg/body weight) for 7 days, groups III and IV rats served as the prophylactic group and were pre-treated with P. longifolia aqueous and methanolic leaf extract for 7 days and then exposed to cadmium for another 7 days, serving as pre-treatment group, groups V, VI, VII, and VIII served as curative groups and were first exposed to cadmium for 7 days and then post-treated with 100 and 200 mg/kg body weight of aqueous extract and 100 and 200 mg/kg body weight of methanolic extract P. longifolia for another 7 days.
Results
Pre- and post-treatment with both extracts of P. longifolia revealed a significant hepatoprotective ability by decreasing the alanine transaminase, alkaline phosphatase, aspartate transaminase, acid phosphatase, gamma glutamyl transferase enzymatic activities were elevated due to cadmium intoxication. Pre- and post-treatment with aqueous and methanolic extract of P. longifolia extract significantly decreased hepatic malondialdehyde levels, together with an improvement in the antioxidant status of superoxide dismutase, glutathione peroxidase, catalase, glutathione S-transferase, and reduced glutathione of rats exposed to cadmium. Histopathology examinations also confirm the above biochemical findings.
Conclusion
The findings from this work suggested that P. longifolia may be beneficial in ameliorating the cadmium-induced hepatotoxicity and oxidative stress in rats.
Author contributions: All the authors read and approved this submitted manuscript. The authors have also accepted responsibility for the entire content of this manuscript.
Research funding: There is none to declare.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization (s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
[1] Santos SD, Verveer PJ, Bastiaens PI. Growth factor-induced MAPK network topology shapes Erk response determining PC-12 cell fate. Nat Cell Biol 2007;9:324–30.10.1038/ncb1543Suche in Google Scholar PubMed
[2] Shati AA, Elsaid FG. Effects of water extracts of thyme (Thymus vulgaris) and ginger (Zingiber officinale Roscoe) on alcohol abuse. Food Chem Toxicol 2009;47:1945–9.10.1016/j.fct.2009.05.007Suche in Google Scholar PubMed
[3] Sarkar A, Ravindran G, Krishnamurthy V. A brief review on the effect of cadmium toxicity from cellular to organ level. Int J Biotech 2013;3:17–36.Suche in Google Scholar
[4] Chatterjee TK. Medicinal plants with hepatoprotective properties: in herbal options, 3rd ed. Calcutta: Books and Allied (P) Ltd., 2000:135.Suche in Google Scholar
[5] Adesanoye OA, Adekunle AE, Adewale OB, Mbagwu AE, Delima AA, Adefegha SA, et al. Chemoprotective effect of Vernonia amygdalina Del. (Astereacea) against 2-acetylaminofluorene-induced hepatotoxicity in rats. Toxicol Indust Health 2016;32:47–58.10.1177/0748233713498436Suche in Google Scholar PubMed
[6] Molehin OR, Oloyede OI, Adefegha SA. Streptozotocin-induced diabetes in rats: effects of white butterfly (Clerodendrum volubile) leaves on blood glucose levels, lipid profile and antioxidant status. Toxicol Mech Meth 2018;28:573–86.10.1080/15376516.2018.1479476Suche in Google Scholar PubMed
[7] Adefegha SA, Omojokun OS, Oboh G. Modulatory effects of protocatechuic acid on cadmium-induced nephrotoxicity and hepatotoxicity in rats in vivo. Springer Plus 2015;4:619.10.1186/s40064-015-1408-6Suche in Google Scholar PubMed PubMed Central
[8] Molehin OR, Oloyede OI, Idowu KA, Adeyanju AA, Olowoyeye AO, Tubi OI, et al. White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats. Biomed Pharmacother 2017;96:924–9.10.1016/j.biopha.2017.12.005Suche in Google Scholar PubMed
[9] Kirtikar KR, Basu BD. Indian medicinal plants Vol. I and II, 3rd Edn., International Book Distributors, Dehradun, 1995:562Suche in Google Scholar
[10] Tanna A, Nair R, Chanda S. Assessment of anti-inflammatory and hepatoprotective potency of Polyalthia longifolia var. pendula leaf in wistar albino rats. J Nat Med 2009;63:80–5.10.1007/s11418-008-0288-2Suche in Google Scholar PubMed
[11] Faizi S, Khan RA, Mughal NR, Malik MS, Sajjadi KE, Ahmad A. Antimicrobial activity of various parts of Polyalthia longifolia isolation of active principles from the leaves and the berries. Phytotherapy Res 2008;22:907–12.10.1002/ptr.2414Suche in Google Scholar PubMed
[12] Murthy MM, Subramanyam M, Bindu MH, Annapurna J. Antimicrobial activity of clerodane diterpenoids from Polyalthia longifolia seeds. Fitoterapia 2005;76:336–9.10.1016/j.fitote.2005.02.005Suche in Google Scholar PubMed
[13] Nair R, Chanda S. Evaluation of polyalthia longifolia (sonn.) thw. leaf extracts for anti-fungal activity. J Cell Tissue Res 2006;6:581–4.Suche in Google Scholar
[14] Nair R, Shukla V, Chanda S. Assessment of Polyalthia longifolia var. pendula for hypoglycemic and antihyperglycemic activity. J Clin Diag Res 2007;1:116–21.Suche in Google Scholar
[15] Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJ. Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals. Indian J Pharmacol 2008;40:126–8.10.4103/0253-7613.42306Suche in Google Scholar PubMed PubMed Central
[16] Farooq T. Phytochemical and pharmacological investigation of the leaves of Carica papaya Linn. Dhaka: East West University, 2009:26–37.Suche in Google Scholar
[17] Debella A. Manual for phytochmical screening of medicinal plants. Addis Ababa, Ethiopia: Department of Drug Research, Ethiopian Health and Nutrition Research Institute, 2002.Suche in Google Scholar
[18] Ojo OA, Ajiboye BO, Oyinloye BE, Ojo AB. Prophylactic effects of ethanolic extract of Irvingia gabonensis stem bark against Cadmium-induced toxicity in albino rats. Adv in Pharmaceutics 2014. DOI: 10.1155/2014/894610.Suche in Google Scholar
[19] European Union. Directive 2010/63/EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. Off J Eur Union. 2010;20:L 276/33–276/ 38.Suche in Google Scholar
[20] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75.10.1016/S0021-9258(19)52451-6Suche in Google Scholar
[21] Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.10.1016/0003-2697(79)90738-3Suche in Google Scholar
[22] Misra HP, Fridovich I. The generation of superoxide radical during the antioxidant of hemoglobin. J Biol Chem 1972;247:6960–2.10.1016/S0021-9258(19)44679-6Suche in Google Scholar
[23] Asru KS. Colorimetric assay of catalase. Anal Biochem 1972;47:389–94.10.1016/0003-2697(72)90132-7Suche in Google Scholar
[24] Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacol 1974;11:151–69.10.1159/000136485Suche in Google Scholar
[25] Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical Role as a Component of Glutathione Peroxidase. Science. 1973;179:588–90. DOI: 10.1126/science.179.4073.588Suche in Google Scholar
[26] Elliott AJ, Scheiber SA, Thomas C, Pardini RS. Inhibition of glutathione reductase by flavonoids:A structure-activity study. Biochemical Pharmacology. 1992 10;44:1603–8. DOI: 10.1016/0006-2952(92)90478-2Suche in Google Scholar
[27] Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130–9.10.1016/S0021-9258(19)42083-8Suche in Google Scholar
[28] Disbrey BD, Rack JH. Book of histological laboratory methods. London: Harcourt Brace/Churchill Livingstone, 1970.Suche in Google Scholar
[29] Betty RK, Jonathan AC. Essential medical statistics, 2nd ed. USA: Blackwell science, 2003:15–409.Suche in Google Scholar
[30] Vincente-Sanchez C, Egido J, Sanchez-Gonzalez PD, Perez- Bariocanal F, Lopez-Novoa JM, Morales AI. Effect of the flavonoid quercetin on cadmium induced hepatotoxicity. Food Chem Toxicol 2008;46:2279–87.10.1016/j.fct.2008.03.009Suche in Google Scholar PubMed
[31] Waisberg M, Joseph P, Hale B, Beyersmann D. Molecular mechanisms of cadmium carcinogenesis. Toxicology 2003;192:95–117.10.1016/S0300-483X(03)00305-6Suche in Google Scholar
[32] Recknagel RO, Glende EA, Dolak JA, Waller RL. Mechanisms of carbon tetrachloride toxicity. Pharmacol Ther 1989;43:139–54.10.1016/0163-7258(89)90050-8Suche in Google Scholar
[33] Nagulendran KR, Velavan S, Mahesh R, Hazeena Begum V. Invitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. Eur J Chem 2007;4:440–9.10.1155/2007/903496Suche in Google Scholar
[34] Nwokocha CR, Nwokocha MI, Owu DU, Obi J, Olatunde B, Ebe C. Comparative analysis on the effect of palm oil (Elaeis guineensis) in reducing cadmium and lead accumulation in liver of Wistar rats. Pharmacognosy Res 2012;4:214–18.10.4103/0974-8490.102266Suche in Google Scholar
[35] Williamson EM, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant material. England: Wiley, 1996:1.Suche in Google Scholar
[36] Morgan NS, ArtavanisTsakonas S, Mooseker MS. Characterization of Drosophila myosin- IA at 31D-F. Mol Biol Cell 1993;4:153a.Suche in Google Scholar
[37] Cui XU, Gu XU, Kang WE. Antioxidant activity in vitro and hepatoprotective effects in vivo of compound lobelia. Altern Med 2016;13:114–22.10.21010/ajtcam.v13i5.15Suche in Google Scholar
[38] Prakash O, Singh GN, Singh RM, Mathur SC, Bajpai M, Yadav S. Protective effects of herbal formula against carbon tetrachloride-induced hepatoxicity. Int J Pharmacol 2008;4:282–6.10.3923/ijp.2008.282.286Suche in Google Scholar
[39] Jain AK, Jain A, Jain A, Jain S, Sikarwar MS, Dubey SK. Hepatoprotective activity of ethanolic extracts of leaves of Polyalthia. Plant Arch. 2006;6:841–2Suche in Google Scholar
[40] Nazima B, Manoharan V, Miltonprabu S. Grape seed proanthocyanidins ameliorates cadmium-induced renal injury. Toxicol Res 2015;3:131–41.10.1039/c3tx50085cSuche in Google Scholar
[41] Asagba SO, Adaikpoh MA, Kadiri H, Obi FO. Influence of aqueous extract of Hibiscus sabdariffa L petal on cadmium toxicity in rats. Biol Trace Elem Res 2007;115:47–57.10.1385/BTER:115:1:47Suche in Google Scholar
[42] Ding WT, Zhang GC, Liu JJ. Truncation of the GPD1 promoter in Saccharomyces cerevisiae for improved ethanol yield and productivity. Appl Environ Microbiol 2013;79:3273–81.10.1128/AEM.03319-12Suche in Google Scholar
[43] Sharma RK, Mandal S, Rajani GP, Gupta N, Srivastava DP. Antiulcer and antiinflammatory activity of fresh leave extracts of Polyalthia Longifolia in rats. Int J Drug Dev Res. 2011;3:351–9Suche in Google Scholar
[44] Afolabi OK, Oyewo EB, Adekunle AS, Adedosu OT, Adedeji AL. Impaired lipid levels and inflammatory response in rats exposed to cadmium. Excli J 2011;11:677–87.Suche in Google Scholar
[45] Soetan KO. Pharmacological and other beneficial effects of antinutritional factors in plants. Afr J Biotechnol 2008;7:4713–21.Suche in Google Scholar
[46] Sunitha S, Nagaraj M, Varalakshmi P. Hepatoprotective effect of lupeol and lupeol linoleate on tissue antioxidant defense system in cadmium-induced hepatotoxicity in rats. Fitoterapia 2001;72:516–23.10.1016/S0367-326X(01)00259-3Suche in Google Scholar
[47] Hossain SK, Bhattacharya S. Prevention of cadmium induced lipid peroxidation, depletion of some antioxidative enzymes and glutathione by a series of novel organoselenocyanates. Environ Toxicol Pharmacol 2006;22:298–308.10.1016/j.etap.2006.04.004Suche in Google Scholar
[48] Pari L, Murugavel P. Role of diallyl tetra sulfide in ameliorating the cadmium induced biochemical changes in rats. Environ Toxicol Pharmacol 2005;20:493–500.10.1016/j.etap.2005.05.009Suche in Google Scholar
[49] Casalino E, Calzaretti G, Sblano C, Landriscina C. Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicol 2002;179:37–50.10.1016/S0300-483X(02)00245-7Suche in Google Scholar
[50] El-Maraghy SA, Gad MZ, Fahim AT, Hamdy MA. Effect of cadmium and aluminum intake on the antioxidant status and lipid peroxidation in rat tissues. J Biochem Mol Toxicol 2001;15:207–14.10.1002/jbt.18Suche in Google Scholar PubMed
© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Reviews
- Music in the workplace: A narrative literature review of intervention studies
- Vitamin C supplementation and C-reactive protein levels: Findings from a systematic review and meta-analysis of clinical trials
- An overview on red algae bioactive compounds and their pharmaceutical applications
- RA-Experimental
- Phenolic composition and antioxidant capacity of hawthorn (Crataegus oxyacantha L.) flowers and fruits grown in Algeria
- Identification of bioactive constituents in Coldenia procumbens L. and its antidiabetic activity against streptozotocin induced Wistar albino rats
- 10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies
- Evaluation of subacute toxicity and herb–drug interaction potential of an herbal Arishta formulation
- Modulatory effect of Polyalthia longifolia leaves against cadmium-induced oxidative stress and hepatotoxicity in rats
- Can measurements be physically conditioned by thought? Further observations following a focused intention experiment
- The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect
- Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats
- Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage
- RA-Clinical
- Ethnobotanical survey of three members of family Lamiaceae among the inhabitants of Bejaia, Northern Algeria
- Effectiveness of video game on bio- physiological parameters during intravenous cannulation among preschool children
- Could Anise decrease the intensity of premenstrual syndrome symptoms in comparison to placebo? A double-blind randomized clinical trial
- Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers
- Effect of yoga practices on general mental ability in urban residential school children
- Perceptions and utilization of traditional healing among Marshallese adults residing in Arkansas
- Effectiveness of neurobic exercise program on memory and depression among elderly residing at old age home
- Does soft tissue mobilization assist static stretching to improve hamstring flexibility? A randomized controlled trial
- Letter to the Editor
- Hot arm and foot bath on heart rate variability and blood pressure in healthy volunteers – needs to be verified with standard device?
Artikel in diesem Heft
- Reviews
- Music in the workplace: A narrative literature review of intervention studies
- Vitamin C supplementation and C-reactive protein levels: Findings from a systematic review and meta-analysis of clinical trials
- An overview on red algae bioactive compounds and their pharmaceutical applications
- RA-Experimental
- Phenolic composition and antioxidant capacity of hawthorn (Crataegus oxyacantha L.) flowers and fruits grown in Algeria
- Identification of bioactive constituents in Coldenia procumbens L. and its antidiabetic activity against streptozotocin induced Wistar albino rats
- 10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies
- Evaluation of subacute toxicity and herb–drug interaction potential of an herbal Arishta formulation
- Modulatory effect of Polyalthia longifolia leaves against cadmium-induced oxidative stress and hepatotoxicity in rats
- Can measurements be physically conditioned by thought? Further observations following a focused intention experiment
- The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect
- Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats
- Interspecific variability of 1,8-cineole content, phenolics and bioactivity among nine Eucalyptus taxa growing under the sub-humid bioclimate stage
- RA-Clinical
- Ethnobotanical survey of three members of family Lamiaceae among the inhabitants of Bejaia, Northern Algeria
- Effectiveness of video game on bio- physiological parameters during intravenous cannulation among preschool children
- Could Anise decrease the intensity of premenstrual syndrome symptoms in comparison to placebo? A double-blind randomized clinical trial
- Epigenetic study of global gene methylation in PON1, XRCC1 and GSTs different genotypes in rural and urban pesticide exposed workers
- Effect of yoga practices on general mental ability in urban residential school children
- Perceptions and utilization of traditional healing among Marshallese adults residing in Arkansas
- Effectiveness of neurobic exercise program on memory and depression among elderly residing at old age home
- Does soft tissue mobilization assist static stretching to improve hamstring flexibility? A randomized controlled trial
- Letter to the Editor
- Hot arm and foot bath on heart rate variability and blood pressure in healthy volunteers – needs to be verified with standard device?