Startseite Molecular insights into endolysosomal microcompartment formation and maintenance
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Molecular insights into endolysosomal microcompartment formation and maintenance

  • Daniel Kümmel EMAIL logo , Eric Herrmann , Lars Langemeyer und Christian Ungermann ORCID logo EMAIL logo
Veröffentlicht/Copyright: 13. Dezember 2022
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 404 Heft 5

Abstract

The endolysosomal system of eukaryotic cells has a key role in the homeostasis of the plasma membrane, in signaling and nutrient uptake, and is abused by viruses and pathogens for entry. Endocytosis of plasma membrane proteins results in vesicles, which fuse with the early endosome. If destined for lysosomal degradation, these proteins are packaged into intraluminal vesicles, converting an early endosome to a late endosome, which finally fuses with the lysosome. Each of these organelles has a unique membrane surface composition, which can form segmented membrane microcompartments by membrane contact sites or fission proteins. Furthermore, these organelles are in continuous exchange due to fission and fusion events. The underlying machinery, which maintains organelle identity along the pathway, is regulated by signaling processes. Here, we will focus on the Rab5 and Rab7 GTPases of early and late endosomes. As molecular switches, Rabs depend on activating guanine nucleotide exchange factors (GEFs). Over the last years, we characterized the Rab7 GEF, the Mon1-Ccz1 (MC1) complex, and key Rab7 effectors, the HOPS complex and retromer. Structural and functional analyses of these complexes lead to a molecular understanding of their function in the context of organelle biogenesis.

Keywords: endosome; GEF; HOPS; lysosome; membrane fusion; tethering
Corresponding authors: Daniel Kümmel, Institute of Biochemistry, University of Münster, Corrensstraße 36, D-48149 Münster, Germany, E-mail: ; and Christian Ungermann, Department of Biology/Chemistry, Biochemistry Section, Osnabrück University, Barbarastraße 13, D-49076 Osnabrück, Germany; and Center of Cellular Nanoanalytics (CellNanOs), Osnabrück University, Barbarastraße 11, D-49076 Osnabrück, Germany, E-mail:

Funding source: Deutsche Forschungsgemeinschaft

Award Identifier / Grant number: SFB 944 - P11

Award Identifier / Grant number: SFB 944 - P17

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was funded by DFG (SFB 944, project P11 to C.U., project P17 to D.K.).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2022-09-28
Accepted: 2022-11-22
Published Online: 2022-12-13
Published in Print: 2023-04-25

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Highlight: Physiology and Dynamics of Cellular Microcompartments
  3. Highlight: on the past and the future of cellular microcompartments
  4. Nuclear redox processes in land plant development and stress adaptation
  5. The readily retrievable pool of synaptic vesicles
  6. Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation
  7. Modulation of self-organizing circuits at deforming membranes by intracellular and extracellular factors
  8. Computational resolution in single molecule localization – impact of noise level and emitter density
  9. Setting up a data management infrastructure for bioimaging
  10. Molecular insights into endolysosomal microcompartment formation and maintenance
  11. The role of lysosomes in lipid homeostasis
  12. Membrane damage and repair: a thin line between life and death
  13. Neuronal stress granules as dynamic microcompartments: current concepts and open questions
  14. Molecular determinants of protein half-life in chloroplasts with focus on the Clp protease system
  15. Neprilysin 4: an essential peptidase with multifaceted physiological relevance
  16. Determinants of synergistic cell-cell interactions in bacteria
  17. Drosophila collagens in specialised extracellular matrices
https://doi.org/10.1515/hsz-2022-0294
Schlagwörter für diesen Artikel
endosome; GEF; HOPS; lysosome; membrane fusion; tethering

Artikel in diesem Heft

  1. Frontmatter
  2. Highlight: Physiology and Dynamics of Cellular Microcompartments
  3. Highlight: on the past and the future of cellular microcompartments
  4. Nuclear redox processes in land plant development and stress adaptation
  5. The readily retrievable pool of synaptic vesicles
  6. Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation
  7. Modulation of self-organizing circuits at deforming membranes by intracellular and extracellular factors
  8. Computational resolution in single molecule localization – impact of noise level and emitter density
  9. Setting up a data management infrastructure for bioimaging
  10. Molecular insights into endolysosomal microcompartment formation and maintenance
  11. The role of lysosomes in lipid homeostasis
  12. Membrane damage and repair: a thin line between life and death
  13. Neuronal stress granules as dynamic microcompartments: current concepts and open questions
  14. Molecular determinants of protein half-life in chloroplasts with focus on the Clp protease system
  15. Neprilysin 4: an essential peptidase with multifaceted physiological relevance
  16. Determinants of synergistic cell-cell interactions in bacteria
  17. Drosophila collagens in specialised extracellular matrices
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