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The readily retrievable pool of synaptic vesicles

  • Sai Krishnan ORCID logo and Jürgen Klingauf EMAIL logo
Published/Copyright: March 6, 2023
Biological Chemistry
From the journal Biological Chemistry Volume 404 Issue 5

Abstract

In the CNS communication between neurons occurs at synapses by secretion of neurotransmitter via exocytosis of synaptic vesicles (SVs) at the active zone. Given the limited number of SVs in presynaptic boutons a fast and efficient recycling of exocytosed membrane and proteins by triggered compensatory endocytosis is required to maintain neurotransmission. Thus, pre-synapses feature a unique tight coupling of exo- and endocytosis in time and space resulting in the reformation of SVs with uniform morphology and well-defined molecular composition. This rapid response requires early stages of endocytosis at the peri-active zone to be well choreographed to ensure reformation of SVs with high fidelity. The pre-synapse can address this challenge by a specialized membrane microcompartment, where a pre-sorted and pre-assembled readily retrievable pool (RRetP) of endocytic membrane patches is formed, consisting of the vesicle cargo, presumably bound within a nucleated Clathrin and adaptor complex. This review considers evidence for the RRetP microcompartment to be the primary organizer of presynaptic triggered compensatory endocytosis.

Keywords: clathrin; exocytosis: endocytosis; RRetP; RRP; synapse
Corresponding author: Jürgen Klingauf, Institute of Medical Physics and Biophysics, University of Münster, Robert-Koch Strasse 31, D-48149, Münster, Germany; and Center for Soft Nanoscience, Busso-Peus Strasse 10, D-48149, Münster, Germany, E-mail:

Funding source: Deutsche Forschungsgemeinschaft

Award Identifier / Grant number: SFB 1348 A02

Award Identifier / Grant number: SFB 944 P5

Acknowledgments

We would like to thank our colleague Dr. Martin Kahms for proofreading this manuscript. This work was supported by grants of the German Research Foundation to JK (DFG: SFB 944 P5 and SFB 1348 A02).

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was financially supported by the Deutsche Forschungsgemeinschaft (SFB 1348 A02, SFB 944 P5).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2022-09-29
Accepted: 2023-02-16
Published Online: 2023-03-06
Published in Print: 2023-04-25

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Highlight: Physiology and Dynamics of Cellular Microcompartments
  3. Highlight: on the past and the future of cellular microcompartments
  4. Nuclear redox processes in land plant development and stress adaptation
  5. The readily retrievable pool of synaptic vesicles
  6. Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation
  7. Modulation of self-organizing circuits at deforming membranes by intracellular and extracellular factors
  8. Computational resolution in single molecule localization – impact of noise level and emitter density
  9. Setting up a data management infrastructure for bioimaging
  10. Molecular insights into endolysosomal microcompartment formation and maintenance
  11. The role of lysosomes in lipid homeostasis
  12. Membrane damage and repair: a thin line between life and death
  13. Neuronal stress granules as dynamic microcompartments: current concepts and open questions
  14. Molecular determinants of protein half-life in chloroplasts with focus on the Clp protease system
  15. Neprilysin 4: an essential peptidase with multifaceted physiological relevance
  16. Determinants of synergistic cell-cell interactions in bacteria
  17. Drosophila collagens in specialised extracellular matrices
https://doi.org/10.1515/hsz-2022-0298
Keywords for this article
clathrin; exocytosis: endocytosis; RRetP; RRP; synapse

Articles in the same Issue

  1. Frontmatter
  2. Highlight: Physiology and Dynamics of Cellular Microcompartments
  3. Highlight: on the past and the future of cellular microcompartments
  4. Nuclear redox processes in land plant development and stress adaptation
  5. The readily retrievable pool of synaptic vesicles
  6. Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation
  7. Modulation of self-organizing circuits at deforming membranes by intracellular and extracellular factors
  8. Computational resolution in single molecule localization – impact of noise level and emitter density
  9. Setting up a data management infrastructure for bioimaging
  10. Molecular insights into endolysosomal microcompartment formation and maintenance
  11. The role of lysosomes in lipid homeostasis
  12. Membrane damage and repair: a thin line between life and death
  13. Neuronal stress granules as dynamic microcompartments: current concepts and open questions
  14. Molecular determinants of protein half-life in chloroplasts with focus on the Clp protease system
  15. Neprilysin 4: an essential peptidase with multifaceted physiological relevance
  16. Determinants of synergistic cell-cell interactions in bacteria
  17. Drosophila collagens in specialised extracellular matrices
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