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The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression

  • Zhijun Liang , Aili Bao , Haixing Li und Hongmei Cai EMAIL logo
Veröffentlicht/Copyright: 8. Januar 2016
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Biologia
Aus der Zeitschrift Biologia Band 70 Heft 10

Abstract

As one of the essential macroelements, nitrogen (N) plays an important role in plant growth and development. In order to know the effect of different N levels on the rice plant growth and carbon-nitrogen metabolism, we analyzed the rice growth phenotype, leaf SPAD value, photosynthesis, carbon-nitrogen metabolic status and gene expression profile under four different N levels (0×N, 0.1×N, 1×N and 5×N). The plant height and dry weight increased with increasing N levels, whereas an opposite trend was observed for the root length, which decreased with increasing N levels. The leaf SPAD value, stem nitrate concentration, soluble proteins, photosynthetic rate, stomatal conductance and total nitrogen concentration increased with increasing N levels, whereas an opposite trend was observed for soluble carbohydrates and carbon/nitrogen ratio which decreased with increasing N levels. Metabolite profile analysis revealed that the low N treatment caused visible decreases in the concentrations of total sugars and organic acids in the leaves, while caused visible increases in the concentrations of total sugars, organic acids and free amino acids in the roots. Gene expression analysis showed that the transcriptional levels of 5 genes (GS1;3, NADH-GOGAT1, NADH-GOGAT2, PEPC4 and PEPC7) altered significantly under four different N levels.

References

Agüera E., Cabello P. & de la Haba P. 2010. Induction of leaf senescence by low nitrogen nutrition in sunflower (Helianthus annuus) plants. Physiol. Plantarum 138: 256-267.10.1111/j.1399-3054.2009.01336.xSuche in Google Scholar

Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Anal. Biochem. 72: 248-254.10.1016/0003-2697(76)90527-3Suche in Google Scholar

Chen X., Cui Z., Vitousek P.M., Cassman K.G., Matson P.A., Bai J., Meng Q., Hou P., Yue S., Römheld V. & Zhang F.2011. Integrated soil-crop system management for food security. Proc. Natl. Acad. Sci. 108: 6399-6404.10.1073/pnas.1101419108Suche in Google Scholar

Coruzzi G.M. & Zhou L. 2001. Carbon and nitrogen sensing and signaling in plants: emerging ‘matrix effects’. Curr. Opin. Plant Biol. 4: 247-253.10.1016/S1369-5266(00)00168-0Suche in Google Scholar

Crawford N.M. & Forde B.G. 2002. Molecular and developmental biology of inorganic nitrogen nutrition. In: Meyerowitz E. & Somerville C. (eds), The Arabidopsis Book, American Society of Plant Biologists, Rockville, MD.10.1199/tab.0011Suche in Google Scholar PubMed PubMed Central

Diaz C., Purdy S., Christ A., Morot-Gaudry J.F., Wingler A. & Masclaux-Daubresse C. 2005. Characterization of markers to determine the extent and variability of leaf senescence in Arabidopsis. A metabolic profiling approach. Plant Physiol. 138: 898-908.10.1104/pp.105.060764Suche in Google Scholar PubMed PubMed Central

Diaz C., Saliba-Colombani V., Loudet O., Belluomo P., Moreau L., Daniel-Vedele F., Morot-Gaudry J.-F. & Masclaux- Daubresse C. 2006. Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana. Plant Cell Physiol. 47: 74-83.10.1093/pcp/pci225Suche in Google Scholar PubMed

Ding L., Wang K.J., Jiang G.M., Biswas D.K., Xu H., Li L.F. & Li Y.H. 2005. Effects of nitrogen deficiency on photosynthetic traits of maize hybrids released in different years. Ann. Bot. (London) 96: 925-930.10.1093/aob/mci244Suche in Google Scholar PubMed PubMed Central

Frink C.R., Waggoner P.E. & Ausubel J.H. 1999. Nitrogen fertilizer: retrospect and prospect. Proc. Natl. Acad. Sci. USA. 96: 1175-1180.10.1073/pnas.96.4.1175Suche in Google Scholar PubMed PubMed Central

Galloway J.N., Townsend A.R., Erisman J.W., Bekunda M., Cai Z., Freney J.R., Martinelli L.A., Seitzinger S.P. & Sutton M.A. 2008. Transformation of the nitrogen cycle: recent trends, questions, and potential solutions. Science 320: 889-892.Suche in Google Scholar

Garnett T., Conn V. & Kaiser B.N. 2009. Root based approaches to improving nitrogen use efficiency in plants. Plant Cell Environ. 32: 1272-1283.10.1111/j.1365-3040.2009.02011.xSuche in Google Scholar PubMed

Gebbing T. & Schnyder H. 1999. Pre-anthesis reserve utilization for protein and carbohydrate synthesis in grains of wheat. Plant Physiol. 121: 871-878.10.1104/pp.121.3.871Suche in Google Scholar PubMed PubMed Central

Gebbing T., Schnyder H. & Kuhbauch W. 1999. The utilization of pre-anthesis reserves in grain filling of wheat: assessment by13C/12C steady-state labelling. Plant Cell Environ. 22: 857-858.10.1046/j.1365-3040.1999.00436.xSuche in Google Scholar

Godfray H.C.J. 2010. Food security: the challenge of feeding 9 billion people. Science 327: 812-818.10.1126/science.1185383Suche in Google Scholar PubMed

Good A.G., Shrawat A.K. & Muench D.G. 2004. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends Plant Sci. 9: 597-605.10.1016/j.tplants.2004.10.008Suche in Google Scholar PubMed

Guo J.H., Liu X.J., Zhang Y., Shen J.L., Han W.X., Zhang W.F., Christie P., Goulding K.W.T., Vitousek P.M. & Zhang F.S. 2010. Significant acidification in major Chinese croplands. Science 327: 1008-1010.10.1126/science.1182570Suche in Google Scholar PubMed

Hirano T., Saito Y., Ushimaru H. & Michiyama H. 2005. The effect of the amount of nitrogen fertilizer on starch metabolism in leaf sheath ofjaponica and indica rice varieties during the heading period. Plant Prod. Sci. 8: 122-130.10.1626/pps.8.122Suche in Google Scholar

Hodge A. 2006. Plastic plants and patchy soils. J. Exp. Bot. 57: 401-411.10.1093/jxb/eri280Suche in Google Scholar PubMed

Krapp A., Ferrario-Mery S. & Touraine B. 2002. Nitrogen and signaling. In: Foyer C. & Noctor G. (eds), Photosynthetic Nitrogen Assimilation and Associated Carbon and Respiratory Metabolism. Advances in Photosynthesis and Respiration, vol 12. Springer, Dordrecht, pp 205-225.Suche in Google Scholar

Krapp A. & Truong H.N. 2005. Regulation of C/N interaction in model plant species. In: Goyal S. Tischner R. & Basra A. (eds), Enhancing the Efficiency of Nitrogen Utilization in Plants. Haworth Press, New York, pp 127-173.Suche in Google Scholar

Kusano M., Fukushima A., Arita M., Jonsson P., Moritz T., Kobayashi M., Hayashi N., Tohge T. & Saito K. 2007a. Unbiased characterization of genotype-dependent metabolic regulations by metabolomic approach in Arabidopsis thaliana. BMC Syst. Biol. 1: 53.10.1186/1752-0509-1-53Suche in Google Scholar PubMed PubMed Central

Kusano M., Fukushima A., Kobayashi M., Hayashi N., Jonsson P., Moritz T., Ebana K. & Saito K. 2007b. Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatographytime-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 855: 71-79.10.1016/j.jchromb.2007.05.002Suche in Google Scholar PubMed

Liu J., You L., Amini M., Obersteiner M., Herreroe M., Zehnder A.J.B. & Yang H. 2010. A high-resolution assessment on global nitrogen flows in cropland. Proc. Natl. Acad. Sci. 107: 8035-8040.10.1073/pnas.0913658107Suche in Google Scholar PubMed PubMed Central

Maness N. 2010. Extraction and analysis of soluble carbohydrates. Methods Mol. Biol. 639: 341-370.10.1007/978-1-60761-702-0_22Suche in Google Scholar PubMed

Martin T., Oswald O. & Graham I.A. 2002. Arabidopsis seedling growth, storage lipid mobilization and photosynthetic gene expression are regulated by carbon: nitrogen availability. Plant Physiol. 128: 472-481.Suche in Google Scholar

Masclaux-Daubresse C., Purdy S., Lema ıtre T., Pourtau N., Taconnat L., Renou J.-P. & Wingler A. 2007. Genetic variation suggests interaction between cold acclimation and metabolic regulation of leaf senescence. Plant Physiol. 143: 434-446.10.1104/pp.106.091355Suche in Google Scholar PubMed PubMed Central

Melo P.M., Lima L.M., Santos I.M., Carvalho H.G. & Cullimore J.V. 2003. Expression of the plastid-located glutamine synthetase of Medicago truncatula: accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids. Plant Physiol. 132: 390-399.10.1104/pp.102.016675Suche in Google Scholar PubMed PubMed Central

Mohd-Radzman N.A., Djordjevic M.A. & Imin N. 2013. Nitrogen modulation of legume root architecture signaling pathways involves phytohormones and small regulatory molecules. Front. Plant Sci. 4: 1-7.10.3389/fpls.2013.00385Suche in Google Scholar PubMed PubMed Central

Morris D.L. 1948 Quantitative determination of carbohydrates with Dreywood’s anthrone reagent. Science 107: 254-255.10.1126/science.107.2775.254Suche in Google Scholar PubMed

Nunes-Nesi A., Fernie A.R. & Stitt M. 2010. Metabolic and signaling aspects underpinning the regulation of plant carbon nitrogen interactions. Mol. Plant 3: 973-996.10.1093/mp/ssq049Suche in Google Scholar PubMed

Pan J., Cui K., Wei D., Huang J., Xiang J. & Nie L. 2011. Relationships of non-structural carbohydrates accumulation and translocation with yield formation in rice recombinant inbred lines under two nitrogen levels. Physiol. Plant. 141: 321-331.10.1111/j.1399-3054.2010.01441.xSuche in Google Scholar PubMed

Redestig H., Fukushima A., Stenlund H., Moritz T., Arita M., Saito K. & Kusano M. 2009. Compensation for systematic cross-contribution improves normalization of mass spectrometry based metabolomics data. Analyt. Chem. 81: 7974-7980.10.1021/ac901143wSuche in Google Scholar PubMed

Robinson D., Hodge A., Griffiths B.S. & Fitter A.H. 1999. Plant root proliferation in nitrogen-rich patches confers competitive advantage. Proc. Biol. Sci. 266: 431-435.10.1098/rspb.1999.0656Suche in Google Scholar

Ruffel S., Krouk G., Ristova D., Shasha D., Birnbaum K.D. & Coruzzi G.M. 2011. Nitrogen economics of root foraging: transitive closure of the nitrate-cytokinin relay and distinct systemic signaling for Nsupplyvs demand. Proc. Natl. Acad. Sci. USA 108: 18524-18529.10.1073/pnas.1108684108Suche in Google Scholar PubMed PubMed Central

Ruuska S.A., Lewis D.C., Kennedy G., Furbank R.T., Jenkins C.L.D. & Tabe L.M. 2008. Large scale transcriptome analysis of the effects of nitrogen nutrition on accumulation of stem carbohydrate in reproductive-stage wheat. Plant Mol. Biol. 66: 15-32.10.1007/s11103-007-9249-5Suche in Google Scholar PubMed

Scofield G.N., Ruuska S.A., Aoki N., Lewis D.C., Tabe L.M. & Jenkins C.L.D. 2009. Starch storage in the stems of wheat plants: localization and temporal changes. Ann. Bot. (London) 103: 859-868.10.1093/aob/mcp010Suche in Google Scholar PubMed PubMed Central

Socolow R. 1999. Nitrogen management and the future of food: Lessons from the management of energy and carbon. Proc. Natl. Acad. Sci. USA 96: 6001-6008.10.1073/pnas.96.11.6001Suche in Google Scholar PubMed PubMed Central

Stitt M., Krapp A. 1999. The interaction between elevated carbon dioxide and nitrogen nutrition: the physiological and molecular background. Plant Cell Envrion. 22: 583-621.10.1046/j.1365-3040.1999.00386.xSuche in Google Scholar

Tilman D., Fargione J., Wolff B., D’Antonio C., Dobson A., Howarth R., Schindler D., Schlesingerm W.H., Simberloff D. & Swackhamer D. 2001. Forecasting agriculturally driven global environmental change. Science 292: 281-284.10.1126/science.1057544Suche in Google Scholar PubMed

Vitousek P.M., Naylor R., Crews T., David M.B., Drinkwater L.E., Holland E., Johnes P.J., Katzenberger J., Martinelli L.A., Matson P.A., Nziguheba G., Ojima D., Palm C.A., Robertson G.P., Sanchez P.A., Townsend A.R. & Zhang F. 2009. Nutrient imbalances in agricultural development. Science 324: 1519-1920.10.1126/science.1170261Suche in Google Scholar PubMed

Yoshida S., Forno D.A., Cook J.H. & Gomez K.A. 1976. Laboratory manual for physiological studies of rice, 3rd ed., International Rice Research Institute, Manila. Walch-Liu P., Ivanov I.I., Filleur S., Gan Y., Remans T. & Forde B.G. 2006. Nitrogen regulation of root branching. Ann. Bot. 97: 875-881.Suche in Google Scholar

Wingler A., Purdy S., Maclean J.A. & Pourtau N. 2006. The role of sugars in integrating environmental signals during the regulation of leaf senescence. J. Exp. Bot. 57: 391-399.10.1093/jxb/eri279Suche in Google Scholar PubMed

Wingler A., von Schaewen A., Leegood R.C., Lea P.J. & Quick W.P. 1998. Regulation of leaf senescence by cytokinin, sugars, and light. Effect on NADH-dependent hydroxypyruvate reductase. Plant Physiol. 116: 329-335.10.1104/pp.116.1.329Suche in Google Scholar

Yoshida S. & Ahn S.B. 1968. The accumulation process of carbohydrate in rice varieties in relation to their response to nitrogen in the tropics. Soil Sci. Plant Nutr. 14: 153-162.10.1080/00380768.1968.10432759Suche in Google Scholar

Yoshida S., Forno D.A., Cook J.H. & Gomez K.A. 1976. Laboratory manual for physiological studies of rice, 3rd ed., International Rice Research Institute, Manila.Suche in Google Scholar

Zhang F,. Cui Z., Fan M., Zhang W., Chen X. & Jiang R. 2011. Integrated soil-crop system management: reducing environmental risk while increasing crop productivity and improving nutrient use efficiency in China. J. Environ. Qual. 40: 1051-1057.10.2134/jeq2010.0292Suche in Google Scholar PubMed

Zhang H., Jennings A., Barlow P.W. & Forde B.G. 1999. Dual pathways for regulation of root branching by nitrate. Proc. Natl. Acad. Sci. USA 96: 6529-6534.10.1073/pnas.96.11.6529Suche in Google Scholar PubMed PubMed Central

Zhang Q. 2007. Strategies for developing green super rice. Proc. Natl. Acad. Sci. USA 104: 16402-16409.10.1073/pnas.0708013104Suche in Google Scholar PubMed PubMed Central

Zheng Z. 2009. Carbon and nitrogen nutrient balance signaling in plants. Plant Signal. Behav. 4: 584-591.10.4161/psb.4.7.8540Suche in Google Scholar PubMed PubMed Central

Zhu Z. & Chen D. 2010. Nitrogen fertilizer use in China - contributions to food production, impacts on the environment and best management strategies. Nutr. Cycl. Agroecosyst. 63: 117-127.Suche in Google Scholar

Received: 2015-1-19
Accepted: 2015-8-27
Published Online: 2016-1-8
Published in Print: 2015-10-1

© 2016

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