Species delimitation and population structure in three Onosma (Boraginaceae) species
-
Masoud Sheidai
,
Samira Sadeghi
Abstract
The present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.
References
Badruddeen R., Sheeba ., Hefazat H., Haque S.E., Khalid M. & Akhtar J. 2012. Psychoimmunomudellatory effects of Onosma bracteatum Wall. (Gaozaban) on stress model in Sprague Dawley rats. J. Clinic. Diag. Res. 6: 1356-1360.Search in Google Scholar
Felsenstein J. 1989. PHYLIP - Phylogeny Inference Package (version 3.2). Cladistics 5: 164-166.Search in Google Scholar
Falush D., Stephens M. & Pritchard J.K. 2007. Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol. Ecol. Notes 7: 574-578.10.1111/j.1471-8286.2007.01758.xSearch in Google Scholar PubMed PubMed Central
Frichot E., Schoville S.D., Bouchard G. & Francois O. 2013. Testing for Associations between Loci and Environmental gradients using latent factor mixed models. Mol. Biol. Evol. 30: 1687-1699.10.1093/molbev/mst063Search in Google Scholar PubMed PubMed Central
Gohari A.R., Saeidneya S., Gohari M.R., Moradi-Afrapoli F., Malmir M., Yazdanpanah M. & Hajiakhoondi A. 2009. Study of the cytotoxic effects of some medicinal plants in Lamiaceae, Asteraceae, Boraginaceaea and Rosaceae on the larva of Artemia salina. J. Med. Plants 29: 87-93.Search in Google Scholar
Hammer O., Harper D.A.T. & Ryan P.D. 2012. “PAST: Paleontological Statistics software package for education and data analysis. Palaeont. Elect. 4: 9.Search in Google Scholar
Holsinger K.E. & Lewis P.O. 2003. Hickory: a package for analysis of population genetic data V1.0. Available at http://www.eeb.uconn.edu; Accessed 2003.Search in Google Scholar
Khatamsaz M. 2002. Flora of Iran (in Farsi), Tehran, Research Institute of Forests and Rangelands.Search in Google Scholar
Kolarčik V., Zozomova-Lihova J. & Martonfi P. 2010. Systematics and evolutionary history of the Asterotricha group of the genus Onosma (Boraginaceae) in central and southern Europe inferred from AFLP and nrDNA ITS data. Plant Syst. Evol. 290: 21-45.10.1007/s00606-010-0346-6Search in Google Scholar
Kolarčik V., Zozomova-Lihova J., Erik Ducar E. & Martonfi P. 2014. Evolutionary significance of hybridization in Onosma (Boraginaceae): analyses of stabilized hemisexual odd polyploids and recent sterile hybrids. Biol. J. Linn. Soci. 112: 89-107.10.1111/bij.12270Search in Google Scholar
Križman M., Jakse J., Baricevic D., Javornik B. & Prošek M. 2006. Robust CTAB activated charcoal protocol for plant DNA extraction. Acta Agri. Sloven. 87: 427-433.Search in Google Scholar
Legendre P. & Makarenkov V. 2002. Reconstruction of biogeographic and evolutionary networks using reticulograms. Syst. Biol. 51: 199-216.10.1080/10635150252899725Search in Google Scholar PubMed
Makarenkov V. & Lapointe F.J. 2004. A weighted least-squares approach for inferring phylogenies from incomplete distance matrices. Bioinformatics 20: 2113-2121.10.1093/bioinformatics/bth211Search in Google Scholar PubMed
Mehrabian A.M., Sheidai M., Noormohammadi Z., Mozafarian V. & Asrei Y. 2012. Palynological diversity in the genus Onosma L. (Boraginaceae) of Iran. Ann. Biol. Res. 3: 3885-3893.Search in Google Scholar
Mehrabian A.R., Sheidai M., Noormohammadi Z., Asrei Y. & Mozafarian V. 2011. Inter-simple sequence repeats (ISSR) and morphological diversity in Onosma L. (Boraginaceae) species in Iran. Afr. J. Biotechnol. 10: 10831-10838.10.5897/AJB11.1910Search in Google Scholar
Mengoni A., Selvi F., Cusimano N., Galardi F. & Gonnelli C. 2006. Genetic diversity inferred from AFLP fingerprinting in populations of Onosma echioides (Boraginaceae) from serpentine and calcareous soils. Plant Biosyst. 140: 211-219.10.1080/11263500600756660Search in Google Scholar
Peakall R. & Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288-295.10.1111/j.1471-8286.2005.01155.xSearch in Google Scholar
Podani J. 2000: Introduction to the Exploration of Multivariate Data (English translation), Leide, Netherlands, Backhuyes.Search in Google Scholar
Perrier X. & Jacquemoud-Collet J.P. 2006. DARwin software version 5·0·155. CIRAD: http://darwin.cirad.fr/darwin .Search in Google Scholar
Pritchard J.K., Stephens M. & Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945-959.10.1093/genetics/155.2.945Search in Google Scholar PubMed PubMed Central
Sheidai M., Seif E., Nouroozi M. & Noormohammadi Z. 2012. Cytogenetic and molecular diversity of Cirsium arvense (Asteraceae) populations in Iran. J. Jpn. Bot. 87: 193-205.Search in Google Scholar
Sheidai M., Zanganeh S., Haji-Ramezanali R., Nouroozi M., Noormohammadi Z. & Ghsemzadeh-Baraki S. 2013. Genetic diversity and population structure in four Cirsium (Asteraceae) species. Biologia 68: 384-397.10.2478/s11756-013-0162-xSearch in Google Scholar
Stebbins G.L. 1993. Concepts of species and genera in flora of North America, pp. 229-246. In: Flora of North America, vol. 1. New York: Oxford University Press.Search in Google Scholar
Suteu D., Popescu F., & Popescu O. 2007. Genetic diversity evaluation of the species Onosma sp. (Boraginaceae). Ann. West Univ. Timisoara Ser. Chem. 16: 45-54.Search in Google Scholar
Suteu D., Băcilă I., Ursu T. & Coldea G. 2012. Genetic diversity assessment of the Romanian populations of Onosma pseudoarenaria and Onosma arenaria. Contributii Botanice 47: 49-57.Search in Google Scholar
Tosun A., Akkol E. K., Bahadir O. & Ye，silada E. 2008. Evaluation of anti-inflammatory and antinociceptive activities of some Onosma L. species growing in Turkey. J. Ethnopharmacol. 120: 378-381.10.1016/j.jep.2008.09.007Search in Google Scholar PubMed
Weising K., Nybom H., Wolff K. & Kahl G. 2005. DNA Fingerprinting in Plants: Principles, Methods, and Applications. Boca Rayton, Fl., USA, CRC Press, 472 pp.10.1201/9781420040043Search in Google Scholar
Yeh F.C. & Boyle T.J.B. 1997. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian J. Bot. 129: 157 Search in Google Scholar
© 2016
Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
