Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
-
Tatjana I. Vujović
,
Đurđina V. Ružić
Abstract
In vitro shoot tips of Serbian autochthonous plums ‘Sitnica’ (Prunus domestica L.) and ‘Crvena Ranka’ (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum ‘Sitnica’, with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. ‘Crvena Ranka’ exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3- 81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.
References
Ashmore S.E. 1997. Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. IPGRI, Rome, 67 pp.Search in Google Scholar
Barraco G., Chatelet P., Balsemin E., Decourcelle T., Sylvestre I. & Engelmann F. 2012. Cryopreservation of Prunus cerasus through vitrification and replacement of cold hardening with preculture on medium enriched with sucrose and/or glycerol. Sci. Hortic. 148: 104-108.10.1016/j.scienta.2012.09.034Search in Google Scholar
Benelli C., De Carlo A. & Engelmann F. 2013. Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol. Adv. 31: 175-185.10.1016/j.biotechadv.2012.09.004Search in Google Scholar
Bortiri E., Oh S.H., Jiang J., Baggett S., Granger A., Weeks C., Buckingham M., Potter D. & Parfitt D.E. 2001. Phylogeny and systematics of Prunus (Rosaceae) as determined by sequence analysis of ITS and the chloroplast trnL-trnF spacer DNA. Syst. Bot. 26: 797-807.Search in Google Scholar
Cruz-Cruz C.A., González Arnao M.T. & Engelmann F. 2013. Biotechnology and conservation of plant biodiversity. Resources 2: 73-95.10.3390/resources2020073Search in Google Scholar
Das B., Ahmed N. & Singh P. 2011. Prunus diversity - early and present development: a review. Int. J. Biodivers. Conserv. 3: 721-734.10.5897/IJBCX11.003Search in Google Scholar
De Boucaud M.T., Brison M., Helliot B. & Hervé-Paulus V. 2002. Cryopreservation of Prunus, pp. 287-311. In: Towill L.E. & Bajaj Y.P.S (eds), Cryopreservation of Plant Germplasm II - Biotechnology in Agriculture and Forestry, Springer-Verlag, Berlin.10.1007/978-3-662-04674-6_21Search in Google Scholar
De Carlo A., Benelli C. & Lambardi M. 2000. Development of a shoot-tip vitrification protocol and comparison with encapsulation-based procedures for plum (Prunus domestica L.) cryopreservation. CryoLetters 21: 215-222.Search in Google Scholar
Engelmann F. 2004. Plant cryopreservation: progress and prospects. In Vitro Cell. Dev. Biol. Plant 40: 427-433.10.1079/IVP2004541Search in Google Scholar
Kaviani B. 2011. Conservation of plant genetic resources by cryopreservation. Aust. J. Crop Sci. 5: 778-800.Search in Google Scholar
Kim H.H., Cho E.G., Baek H.J., Kim C.Y., Keller E.R.J. & Engelmann F. 2004. Cryopreservation of garlic shoot tips by vitrification: effects of dehydration, rewarming, unloading and regrowth conditions. CryoLetters 25: 59-70.Search in Google Scholar
Kim H.H., Lee Y.G., Park S.U., Lee S.C., Baek H.J., Cho E.G. & Engelmann F. 2009a. Development of alternative loading solutions in droplet-vitrification procedures. CryoLetters 30: 291-299.Search in Google Scholar
Kim H.H., Lee Y.G., Shin D.J., Ko H.C., Gwag J.G., Cho E.G. & Engelmann F. 2009b. Development of alternative plant vitrification solutions in droplet-vitrification procedures. CryoLetters 30: 320-334.Search in Google Scholar
Kryszczuk A., Keller J., Gr¨ube M. & Zimnoch-Guzowska E. 2006. Cryopreservation of potato (Solanum tuberosum L.) shoot tips using vitrification and droplet method. J. Food Agric Environ. 4: 196-200.Search in Google Scholar
Murashige T. & Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.10.1111/j.1399-3054.1962.tb08052.xSearch in Google Scholar
Nenadović-Mratinić E., Nikićević N., Milatović D. & Đurović D. 2007. Suitability of autochthonous plum cultivars (Prunus insititia L.) for brandy production. J. Pomology 41: 159-164.Search in Google Scholar
Nishizawa S., Sakai A., Amano Y. & Matsuzawa T. 1993. Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Sci. 91: 67-73.10.1016/0168-9452(93)90189-7Search in Google Scholar
Panis B. & Lambardi M. 2006. Status of cryopreservation technologies in plants (crops and forest trees), pp. 43-54. In: Ruane J. & Sonnino A. (eds), The Role of Biotechnology in Exploring and Protecting Agricultural Genetic Resources, Food and Agricultural Organization of the United Nations, Rome.Search in Google Scholar
Panis B., Piette B., André E., Van den Houwe I. & Swennen R. 2011. Droplet vitrification: the first generic cryopreservation protocol for organized plant tissues? Acta Hort. 908: 157-162.10.17660/ActaHortic.2011.908.17Search in Google Scholar
Preetha T.S., Hemanthakumar A.S. & Krishnan P.N. 2013. Shoot tip cryopreservation by vitrification in Kaempferia galanga L. an endangered, overexploited medicinal plant in Tropical Asia. IOSR J. Pharm. Biol. Sci. 8: 19-23.Search in Google Scholar
Ružić Đ., Vujović T. & Cerović R. 2008. Propagation of autochthonous plum Sitnica (Prunus domestica L.) by micropropagation in vitro. J. Pomology 42: 103-109.Search in Google Scholar
Ružić Dj., Vujović T. & Cerović R. 2013. Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus × Prunus canescens) shoot tips by droplet-vitrification technique. J. Hortic. Res. 21: 79-85.10.2478/johr-2013-0025Search in Google Scholar
Ružić Dj., Vujović T. & Cerović R. 2014. Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. Hort. Sci. 41: 55-63.10.17221/234/2013-HORTSCISearch in Google Scholar
Sakai A. & Engelmann F. 2007. Vitrification, encapsulationvitrification and droplet-vitrification: a review. CryoLetters 28: 151-157.Search in Google Scholar
Shatnawi M.A., Shibli R., Qrunfleh I., Bataeineh K. & Obeidat M. 2007. In vitro propagation and cryopreservation of Prunus avium using vitrification and encapsulation dehydration methods. J. Food Agric. Environ. 5: 204-208.Search in Google Scholar
Vujović T., Sylvestre I., Ružić Đ. & Engelmann F. 2011. Dropletvitrification of apical shoot tips of Rubus fruticosus L. and Prunus cerasifera Ehrh. Sci. Hortic. 130: 222-228.10.1016/j.scienta.2011.06.049Search in Google Scholar
Zhao Y., Wu Y., Chen H., Li C., Chen X. & Lu X. 2008. Cryopreservation of shoot tips from Prunus. Acta Hort. Sinica 35: 423-426.Search in Google Scholar
© 2016
Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
