Home Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
Article
Licensed
Unlicensed Requires Authentication

Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities

  • Agbaje Lateef EMAIL logo , Sunday A. Ojo , Abiola S. Akinwale , Luqmon Azeez , Evariste B. Gueguim-Kana and Lorika S. Beukes
Published/Copyright: January 8, 2016
Become an author with De Gruyter Brill
Author Information Explore this Subject
Biologia
From the journal Biologia Volume 70 Issue 10

Abstract

The cell-free extract of Bacillus safensis LAU 13 strain (GenBank accession No: KJ461434) was used for green biosynthesis of silver nanoparticles (Ag-NPs). Characterization of Ag-NPs was carried out using UV-VIS spectroscopy, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Evaluation of synthesized Ag-NPs as antimicrobial agents was done using multi-drug resistant clinical isolates as well as their synergistic effects when combined with some selected antibiotics. Furthermore, potential of Ag-NPs as antimicrobial additives in paint was demonstrated. The Ag-NPs have maximum absorbance at 419 nm, with peaks at 3308, 2359, 1636, and 422 cm−1, indicating that proteins were the capping and stabilisation molecules in the synthesis of Ag-NPs. The particles were spherical shaped having size of 5-95 nm, with silver as the prominent metal from the energy dispersive X-ray analysis, while selected area electron diffraction pattern agrees well with the crystalline nature and face-centred cubic phase of Ag-NPs. Inhibition of Staphylococcus aureus, Escherichia coli, Klebsiella granulomatis and Pseudomonas aeruginosa was achieved at 100 μg/mL. Improvement of activities of augmentin, ofloxacin and cefixime to the tune of 7.4-142.9% was achieved in synergistic study, while total inhibitions of P. aeruginosa, S. aureus, Aspergillus flavus and Aspergillus fumigatus were achieved in Ag-NPs-paint admixture. The Ag-NPs showed potent antioxidant and larvicidal activities with IC50 and LC50 of 15.99 and 42.19 μg/mL, respectively. The present study demonstrated that the biosynthesized Ag-NPs have potent biological activities, which can find applications in diverse areas. The report adds to the growing biotechnological relevance of B. safensis.

Keywords: Bacillus safensis; silver nanoparticles; antimicrobial activity; multi-drug resistance; paint; antioxidant; larvicidal activity

References

Adewoye S.O. & Lateef A. 2004. Assessment of the microbiological quality of Clarias gariepinus exposed to an industrial effluent in Nigeria. Environmentalist 24: 249-254.10.1007/s10669-005-1000-7Search in Google Scholar

Andrews J.M. 2005. BSAC standardized disc susceptibility testing method (version 4). J. Antimicrob. Chemother. 56: 60-76.10.1093/jac/dki124Search in Google Scholar PubMed

Bhakya S., Muthukrishnan S., Sukumaran M. & Muthukumar M. 2015. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Appl. Nanosci. (in press) http://dx.doi.org.10.1007/s13204-015-0473-z.10.1007/s13204-015-0473-zSearch in Google Scholar

Bhat R., Desphande R., Ganachari S.V., Huh D.O. & Venkarataman A. 2011. Photo-irradiated bio-synthesis of silver nanoparticles using edible mushroom Pleurotus florida and their antibacterial activity studies. Bioinorg. Chem. Applic. 2011: Article ID: 650979.10.1155/2011/650979Search in Google Scholar PubMed PubMed Central

Cho K.H., Park J.E., Osaka T. & Park S.G. 2005. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim. Acta 51: 956-960.10.1016/j.electacta.2005.04.071Search in Google Scholar

Chortyk T.O., Severson R.F., Cutler H.C. & Siesson V.A. 1993.Antibiotic activities of sugar esters isolated from selected Nicotiana species. Biosci. Biotechnol. Biochem. 57: 1355-1356.10.1271/bbb.57.1355Search in Google Scholar PubMed

Devi L.S. & Joshi S.R. 2012. Antimicrobial and synergistic effects of silver nanoparticles synthesized using soil fungi of high altitudes of Eastern Himalaya. Mycobiology 40: 27-34.10.5941/MYCO.2012.40.1.027Search in Google Scholar PubMed PubMed Central

El-Batal A.I., ElKenawya N.M., Yassin A.S. & Amin M.A. 2015. Laccase production by Pleurotus ostreatus and its application in synthesis of gold nanoparticles. Biotechnol. Reports 5: 31-39.10.1016/j.btre.2014.11.001Search in Google Scholar PubMed PubMed Central

El-Shanshoury A.E.R., ElSilk S.E. & Ebeid M.E. 2011. Extracellular biosynthesis of silver nanoparticles using Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, and Streptococcus thermophilus Esh1 and their antimicrobial activities. ISRN Nanotechnology 2011: Article ID: 385480.Search in Google Scholar

Fayaz A.M., Balaji K., Girilal M., Yadav R., Kalaichelvam P.T. & Venketesan R. 2010. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed. Nanotechnol. Biol. Med. 6: 103-109.10.1016/j.nano.2009.04.006Search in Google Scholar PubMed

Goncharova A.V., Karpenyuk T.A., Tsurkan Y.S., Beisembaeva R.U., Kalbaeva A.M., Mukasheva T.D. & Ignatova L.V. 2013.Screening and identification of microorganisms-potential producers of arachidonic acid. Int. J. Biol. Agric. Biosystems Life Sci. Eng. 7: 368-371.Search in Google Scholar

Jayanthi P. & Lalitha P. 2011. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int. J. Pharm. Pharmaceut. Sci. 3: 126-128.Search in Google Scholar

Kaiser J.P., Zuin S. & Wick P. 2013. Is nanotechnology revolutionizing the paint and lacquer industry? A critical opinion. Sci. Total Environ. 442: 282-289.10.1016/j.scitotenv.2012.10.009Search in Google Scholar PubMed

Kannan R.R.R., Arumugam R., Ramya D., Manivannan K. & Anantharaman P. 2013. Green synthesis of silver nanoparticles using marine macroalga Chaetomorpha linum. Appl. Nanosci. 3: 229-233.10.1007/s13204-012-0125-5Search in Google Scholar

Khaneja R., Perez-Fons L., Fakhry S., Baccigalupi L., Steiger S., To E., Sandmann G., Dong T.C., Ricca E., Fraser P.D. & Cutting S.M. 2010. Carotenoids found in Bacillus. J. Appl. Microbiol. 108: 1889-1902.10.1111/j.1365-2672.2009.04590.xSearch in Google Scholar PubMed

Kothari V.V., Kothari R.K., Kothari C.R., Bhatt V.D., Nathani N.M., Koringa P.G., Joshi C.G. & Vyas B.R.M. 2013. Genomic sequence of salt-tolerant Bacillus safensis strain VK, isolated from saline desert area of Gujarat, India. Genome Announc. 2: e00337-14.Search in Google Scholar

Lateef A. 2004. The microbiology of a pharmaceutical effluent and its public health implications.World J. Microbiol. Biotechnol. 20: 167-171.10.1023/B:WIBI.0000021752.29468.4eSearch in Google Scholar

Lateef A., Adelere I.A. & Gueguim-Kana E.B. 2015a. Bacillus safensis LAU 13: a new source of keratinase and its multifunctional biocatalytic applications. Biotechnol. Biotechnol. Equip. 29: 54-63.10.1080/13102818.2014.986360Search in Google Scholar PubMed PubMed Central

Lateef A., Adelere I.A. & Gueguim-Kana E.B. 2015b. The biology and potential biotechnological applications of Bacillus safensis. Biologia 70: 411-419.10.1515/biolog-2015-0062Search in Google Scholar

Lateef A., Adelere I.A., Gueguim-Kana E.B., Asafa T.B. & Beukes L.S. 2015c. Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13. Int. Nano Lett. 5: 29-35.10.1007/s40089-014-0133-4Search in Google Scholar

Lateef A., Azeez M.A., Asafa T.B., Yekeen T.A., Akinboro A., Oladipo I.C., Ajetomobi F.E., Gueguim-Kana E.B. & Beukes, L.S. 2015d. Cola nitida-mediated biogenic synthesis of silver nanoparticles using seed and seed shell extracts and evaluation of antibacterial activities. BioNanoSci. (in press) http://dx.doi.org.10.1007/s12668-015-0181-x.10.1007/s12668-015-0181-xSearch in Google Scholar

Lateef A., Davies T.E., Adelekan A., Adelere I.A., Adedeji A.A. & Fadahunsi A.H. 2010. Akara Ogbomoso: microbiological examination and identification of hazards and critical control points. Food Sci. Technol. Int. 16: 389-400.10.1177/1082013210366894Search in Google Scholar PubMed

Lateef A. & Ojo M.O. 2015. Public health issues in the processing of cassava (Manihot esculenta) for the production of lafun and the application of hazard analysis control measures. Qual. Assur. Safety Crops Foods (in press) http://dx.doi.org.10.3920/QAS2014.0476.10.3920/QAS2014.0476Search in Google Scholar

Lateef A., Ojo S.A., Azeez M.A., Asafa T.B., Yekeen T.A., Akinboro A., Oladipo I.C., Gueguim-Kana E.B. & Beukes L.S. 2015e. Cobweb as novel biomaterial for the green and ecofriendly synthesis of silver nanoparticles. Appl. Nanosci. (in press) http://dx.doi.org.10.1007/s13204-015-0492-9.10.1007/s13204-015-0492-9Search in Google Scholar

Lateef A., Oloke J.K. & Gueguim-Kana E.B. 2004. Antimicrobial resistance of bacterial strains isolated from orange juice products. Afr. J. Biotechnol. 3: 334-338.10.5897/AJB2004.000-2061Search in Google Scholar

Lateef A., Oloke J.K. & Gueguim-Kana E.B. 2005. The prevalence of bacterial resistance in clinical, food, water and some environmental samples in Southwest Nigeria. Environ. Monit. Assess. 100: 59-69.Search in Google Scholar

Lateef A., Oloke J.K., Gueguim Kana E.B., Oyeniyi S.O., Onifade O.R., Oyeleye A.O., Oladosu O.C. & Oyelami A.O. 2008. Improving the quality of agro-wastes by solid state fermentation: enhanced antioxidant activities and nutritional qualities. World J. Microbiol. Biotechnol. 24: 2369-2374.10.1007/s11274-008-9749-8Search in Google Scholar

Lateef A., Oloke J.K., Gueguim-Kana E.B. & Pacheco E. 2006. The microbiological quality of ice used to cool drinks and foods in Ogbomoso metropolis, Southwest, Nigeria. Internet J. Food Safety 8: 39-43.Search in Google Scholar

Lateef A. & Yekeen T.A. 2006. Microbial attributes of a pharmaceutical effluent and its genotoxicity on Allium cepa. Int. J. Environ. Stud. 63: 534-536.Search in Google Scholar

Lateef A., Yekeen T.A. & Ufuoma P.E. 2007. Bacteriology and genotoxicity of some pharmaceutical wastewaters in Nigeria. Int. J. Environ. Health 1: 551-562. Mishra A., Kumari M., Pandey S., Chaudhry V., Gupta K.C. & Nautiyal C.S. 2014. Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by Trichoderma sp. Bioresour. Technol. 166: 235-242.Search in Google Scholar

Mokhtari M., Deneshpojouh S., Seyedbagheri S., Atashdehghan R., Abdi K., Sarkar S. & Sharverdi R.A. 2009. Biological synthesis of very small silver nanoparticles by culture supernatant of Klebsiella pneumoniae: the effect of visible-light irradiation and the liquid mixing process. Mater. Res. Bull. 44: 1415-1421.10.1016/j.materresbull.2008.11.021Search in Google Scholar

Nazeruddin G.M., Prasad N.R., Prasad S.R., Shaikh Y.I., Waghmare S.R. & Adhyapak P. 2014. Coriandrum sativum seed extract assisted in situ green synthesis of silver nanoparticle and its anti-microbial activity. Ind. Crops Prod. 60: 212-216.10.1016/j.indcrop.2014.05.040Search in Google Scholar

Olajire A.A. & Azeez L. 2011. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. Afr. J. Food Sci. Technol. 2: 022-029.Search in Google Scholar

Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315.10.5264/eiyogakuzashi.44.307Search in Google Scholar

Patil C.D., Borase H.P., Patil S.V., Salunkhe R.B. & Salunke B.K. 2012. Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and non-target fish Poecillia reticulata. Parasitol. Res. 111: 555-562.10.1007/s00436-012-2867-0Search in Google Scholar PubMed

Perez C., Paul M. & Bazerque P. 1990. Antibiotic assay by agar well diffusion method. Acta Biol. Med. Exp. 15: 113-115.Search in Google Scholar

Porob S., Nayak S., Fernandes A., Padmanabhan P., Patil B.A., Meena R.M. & Ramaiah N. 2013. PCR screening for the surfactin (sfp) gene in marine Bacillus strains and its molecular characterization from Bacillus tequilensis NIO11. Turk. J. Biol. 37: 212-221.10.3906/biy-1206-40Search in Google Scholar

Priyadarshini K.A., Murugan K., Panneerselvam C., Ponarulselvam S., Hwang J.S. & Nicoletti M. 2012. Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res. 111: 997-1006.10.1007/s00436-012-2924-8Search in Google Scholar PubMed

Priyadarshini S., Gopinath V., Priyadharsshini N.M., Ali D.M. & Velusamy P. 2013. Synthesis of anisotropic silver nanoparticles using novel strain, Bacillus flexus and its application. Colloids Surf. B Biointerfaces 102: 232-237.Search in Google Scholar

Raja C.E. & Omine K. 2012. Arsenic, boron and salt resistant Bacillus safensis MS11 isolated from Mongolia desert soil. Afr. J. Biotechnol. 11: 2267-2275.Search in Google Scholar

Rajarathinam M., Dhanpal D., Morukattu G., Joseph S. & Thangavelu K.P. 2014. Imparting potential antimicrobial and antifungal activities to water based interior paint using nanoparticles of silver as an additive - an eco-friendly approach. Adv. Sci. Eng. Med. 6: 676-682.10.1166/asem.2014.1550Search in Google Scholar

Raliya R. & Tarafdar J.C. 2014. Biosynthesis and characterization of zinc, magnesium and titanium nanoparticles: an ecofriendly approach. Int. Nano Lett. 4: 1-10.Search in Google Scholar

Reza K.M., Ashrafalsadat N., Reza R.M., Taher N. & Ali N. 2014. Isolation and molecular identification of extracellular lipase-producing Bacillus species from soil. Annals Biol. Res. 5: 132-139.Search in Google Scholar

Roohi A., Ahmed I., Khalid N., Iqbal M. & Jamil M. 2014. Isolation and phylogenetic identification of halotolerant/halophilic bacteria from the salt mines of Karak, Pakistan. Int. J. Agric. Biol. 16: 564-570.Search in Google Scholar

Roopan S.M., Madhumitha G., Rahuman A.A., Kamaraj C., Bharathi A. & Surendra T.V. 2013. Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Ind. Crops Prod. 43: 631-635.10.1016/j.indcrop.2012.08.013Search in Google Scholar

Salem W.M., Haridy M., Sayed W.F. & Hassan N.H. 2014. Antibacterial activity of silver nanoparticles synthesized from latex and leaf extract of Ficus sycomorus. Ind. Crops Prod. 62: 228-234.10.1016/j.indcrop.2014.08.030Search in Google Scholar

Samadi N., Golkaran D., Eslamifar A., Jamalifar H., Fazeli M.R. & Mohseni F.A. 2009. Intra/extracellular biosynthesis of silver nanoparticles by an autochtonous strain of Proteus mirabilis isolated from photographic waste. J. Biomed. Nanotechnol. 5: 247-253 Satomi M., Myron T., Duc L. & Venkateswaran K. 2006. Bacillus safensis sp. nov., isolated from spacecraft and assemblyfacility surfaces. Int. J. Syst. Evol. Microbiol. 56: 1735-1740.Search in Google Scholar

Shameli K., Ahmad M.B., Zargar M., Wan Yunus W.M.Z., Ibrahim N.A., Sha-banzadeh P. & Ghaffari-Moghadam M. 2011. Synthesis and characterization of silver/montmorillonite/ chitosan bionanocomposites by chemical reduction method and their antibacterial activity. Int. J. Nanomed. 6: 271-284.10.2147/IJN.S16043Search in Google Scholar PubMed PubMed Central

Shankar S., Jaiswal L., Aparna R.S.L. & Prasad R.G.S.V. 2014.Search in Google Scholar

Synthesis, characterization, in vitro biocompatibility, and antimicrobial activity of gold, silver and gold silver alloy nanoparticles prepared from Lansium domesticum fruit peel extract. Mater. Lett. 137: 75-78.10.1016/j.matlet.2014.08.122Search in Google Scholar

Shanmugam C., Sivasubramanian G., Parthasarathi B., Baskaran K., Balachander R. & Parameswaran V.R. 2015. Antimicrobial, free radical scavenging activities and catalytic oxidation of benzyl alcohol by nano-silver synthesized from the leaf extract of Aristolochia indica L.: a promenade towards sustainability. Appl. Nanosci. (in press) http://dx.doi.org.10.1007/ s13204-015-0477-8. Search in Google Scholar

Received: 2015-9-14
Accepted: 2015-10-16
Published Online: 2016-1-8
Published in Print: 2015-10-1

© 2016

Articles in the same Issue

  1. An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
  2. A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
  3. Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
  4. Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
  5. Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
  6. Species delimitation and population structure in three Onosma (Boraginaceae) species
  7. Glycinebetaine priming improves salt tolerance of wheat
  8. The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
  9. High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
  10. Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
  11. Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
  12. Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
  13. New and firstly recorded oribatid mites from Turkey
  14. Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
  15. A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
  16. Skull variability of mice and voles inhabiting the territory of a great cormorant colony
  17. High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
  18. Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
  19. Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
https://doi.org/10.1515/biolog-2015-0164
Keywords for this article
Bacillus safensis; silver nanoparticles; antimicrobial activity; multi-drug resistance; paint; antioxidant; larvicidal activity

Articles in the same Issue

  1. An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
  2. A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
  3. Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
  4. Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
  5. Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
  6. Species delimitation and population structure in three Onosma (Boraginaceae) species
  7. Glycinebetaine priming improves salt tolerance of wheat
  8. The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
  9. High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
  10. Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
  11. Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
  12. Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
  13. New and firstly recorded oribatid mites from Turkey
  14. Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
  15. A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
  16. Skull variability of mice and voles inhabiting the territory of a great cormorant colony
  17. High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
  18. Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
  19. Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 21.10.2025 from https://www.degruyterbrill.com/document/doi/10.1515/biolog-2015-0164/html
Scroll to top button