Startseite Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
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Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities

  • Agbaje Lateef EMAIL logo , Sunday A. Ojo , Abiola S. Akinwale , Luqmon Azeez , Evariste B. Gueguim-Kana und Lorika S. Beukes
Veröffentlicht/Copyright: 8. Januar 2016
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Biologia
Aus der Zeitschrift Biologia Band 70 Heft 10

Abstract

The cell-free extract of Bacillus safensis LAU 13 strain (GenBank accession No: KJ461434) was used for green biosynthesis of silver nanoparticles (Ag-NPs). Characterization of Ag-NPs was carried out using UV-VIS spectroscopy, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Evaluation of synthesized Ag-NPs as antimicrobial agents was done using multi-drug resistant clinical isolates as well as their synergistic effects when combined with some selected antibiotics. Furthermore, potential of Ag-NPs as antimicrobial additives in paint was demonstrated. The Ag-NPs have maximum absorbance at 419 nm, with peaks at 3308, 2359, 1636, and 422 cm−1, indicating that proteins were the capping and stabilisation molecules in the synthesis of Ag-NPs. The particles were spherical shaped having size of 5-95 nm, with silver as the prominent metal from the energy dispersive X-ray analysis, while selected area electron diffraction pattern agrees well with the crystalline nature and face-centred cubic phase of Ag-NPs. Inhibition of Staphylococcus aureus, Escherichia coli, Klebsiella granulomatis and Pseudomonas aeruginosa was achieved at 100 μg/mL. Improvement of activities of augmentin, ofloxacin and cefixime to the tune of 7.4-142.9% was achieved in synergistic study, while total inhibitions of P. aeruginosa, S. aureus, Aspergillus flavus and Aspergillus fumigatus were achieved in Ag-NPs-paint admixture. The Ag-NPs showed potent antioxidant and larvicidal activities with IC50 and LC50 of 15.99 and 42.19 μg/mL, respectively. The present study demonstrated that the biosynthesized Ag-NPs have potent biological activities, which can find applications in diverse areas. The report adds to the growing biotechnological relevance of B. safensis.

Keywords: Bacillus safensis; silver nanoparticles; antimicrobial activity; multi-drug resistance; paint; antioxidant; larvicidal activity

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Received: 2015-9-14
Accepted: 2015-10-16
Published Online: 2016-1-8
Published in Print: 2015-10-1

© 2016

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https://doi.org/10.1515/biolog-2015-0164
Schlagwörter für diesen Artikel
Bacillus safensis; silver nanoparticles; antimicrobial activity; multi-drug resistance; paint; antioxidant; larvicidal activity

Artikel in diesem Heft

  1. An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
  2. A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
  3. Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
  4. Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
  5. Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
  6. Species delimitation and population structure in three Onosma (Boraginaceae) species
  7. Glycinebetaine priming improves salt tolerance of wheat
  8. The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
  9. High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
  10. Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
  11. Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
  12. Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
  13. New and firstly recorded oribatid mites from Turkey
  14. Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
  15. A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
  16. Skull variability of mice and voles inhabiting the territory of a great cormorant colony
  17. High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
  18. Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
  19. Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
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