Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
-
Aneta Štochmačová
,
Attila Kádasi
Abstract
Rooibos is widely used in folk medicine, but its influence on reproductive processes has been studied insufficiently. The aim of present study was to examine possible involvement of rooibos on the release of progesterone (P4), testosterone (T) and insulin-like growth factor I (IGF-I), as well as its response to gonadotropin - follicle-stimulating and luteinizing hormone (FSH+LH), the most known hormonal regulators for reproduction. Rabbit ovarian fragments were cultured in the presence of rooibos (1, 10 and 100 μg/mL medium), FSH+LH (0.01, 0.1 and 1 IU/mL medium) and rooibos (10 μg/mL medium) in combination with FSH+LH (0.01, 0.1 and 1 IU/mL medium). The release of hormones was evaluated by using radioimmunoassay. It was observed that rooibos addition to culture medium stimulated P4 and inhibited T release. IGF-I release was significantly stimulated by rooibos at all doses added. FSH+LH when given alone promoted IGF-I, but not P4 or T release. The addition of rooibos induced the suppressive effect of FSH+LH on P4 release, the stimulatory action of FSH+LH on T output and reduced the response of IGF-I to FSH+LH treatment. These data suggest that rooibos is able to directly affect the release of steroid (P4 and T) and peptide (IGF-I) hormones by rabbit ovarian cells and to modulate their response to gonadotropins.
References
Agarwal A., Sajal G. & Sharma R.K. 2005. Role of oxidative stress in female reproduction. Reprod. Biol. Endocrinol. 3: 28.10.1186/1477-7827-3-28Search in Google Scholar
Awonyi D.O., Aboua Y.G., Marnewick J. & Brooks N. 2012. The effects of rooibos (Aspalathus linearis), green tea (Camellia sinensis) and commercial rooibos and green tea supplements on epididymal sperm in oxidative stress-induced rats. Phytother. Res. 26: 1231-1239.Search in Google Scholar
Bramati L., Aquilano F. & Pietta P. 2002. Unfermented rooibos tea: quantitative characterization of flavonoids by HPLC-UV and determination of the total antioxidant activity. J. Agric. Food Chem. 51: 72-74.10.1021/jf0347721Search in Google Scholar
Duke J.A., Bogenschutz-Godwin M.J., Ducelliar J. & Duke P.A.K. 2002. Handbook of Medicinal Herbs, 2nd Edn., CRC Press, Boca Raton.10.1201/9781420040463Search in Google Scholar
Fang J., Zhou Q., Shi X.L. & Jiang B.H. 2006. Luteolin inhibits insulin-like growth factor 1 receptor signaling in prostate cancer cells. Carcenogenesis 28: 713-723.10.1093/carcin/bgl189Search in Google Scholar
Gilani A.H., Shah A.J., Ghayur M.N. & Majeed K. 2005. Pharmacological basis for the use of tumeric in gastrointestinal and respiratory disorders. Life Sci. 76: 3089-3105.10.1016/j.lfs.2004.12.021Search in Google Scholar
Gonzales-Robayna I.J., Falender A.E., Ochsner S., Firestone G.L. & Richards J.S. 2000. Follicle-stimulating hormone (FSH) stimulates phosphorylation and activation of protein kinase B (PKB/Akt) and serum and glucocorticoid-induced kinase (Sgk): evidence for A kinase-independent signaling by FSH in granulosa cells. Mol. Endocrinol. 14: 1283-1300.10.1210/mend.14.8.0500Search in Google Scholar
Hagan C.R., Faivre E.J. & Lange C.A. 2009. Scaffolding actions of membrane-associated progesterone receptors. Steroids 74: 568-572.10.1016/j.steroids.2008.12.004Search in Google Scholar
Inanami O., Asanuma T., Inukai N., Jin T., Shimokawa S., Kasai N., Nakano M., Sato F. & Kuwabara M. 1995. The suppression of age-related accumulation of lipis peroxides in rat brain by administration of Rooibos tea (Aspalathus linearis). Neurosci Lett. 196: 85-88.10.1016/0304-3940(95)11853-OSearch in Google Scholar
Joubert E., Winterton P., Britz T.J. & Ferreira D. 2004. Superoxide anion and α,α-diphenyl-β-picrylhydrazyl radical scavenging capacity of rooibos (Aspalathus linearis) aqueous extracts, crude phenolic fractions, tannin and flavonoids. Food Res. Int. 37: 133-138.10.1016/j.foodres.2003.09.011Search in Google Scholar
Jurani M., Lamošova D., Mačajova M., Košťal Ľ., Joubert E. & Greksak M. 2008. Effect of rooibos tea (Aspalthus linearis) on Japanese quail growth, egg production and plasma metabolites. Br. Poult. Sci. 49: 55-64.Search in Google Scholar
Kayampilly P.P. & Menon K. 2004. Inhibition of extracellular signal-regulated protein kinase-2 phosphorylation by dihydrotestoterone reduces follicle stimulating hormone mediated cyclin D2 messenger ribonucleic acid expression in rat granulosa cells. Endocrinology 145: 1786-1793.10.1210/en.2003-1029Search in Google Scholar PubMed
Marnewick J., Joubert E., Joseph S., Swanevelder S., Swart P. & Gelderbolm W. 2005. Inhibition of tumour promotor in mouse skin by extracts of rooibos (Aspalathus linearis) and honeybush (Cyclopia intermedia), unique Southern African herbal teas. Cancer Lett. 224: 193-202.Search in Google Scholar
Marnewick J.L., Rautenbach F., Venter I., Neethling H., Blackhurst D.M., Wolmarans P. & Macharia M. 2011. Effects of rooibos (Aspalathus linearis) on oxidative stress and biochemical parameters in adults at risk for cardiovascular disease. J. Ethnopharmacol. 133: 46-52.10.1016/j.jep.2010.08.061Search in Google Scholar PubMed
McGarvey C., Cates P.S., Brooks A.N., Swanson I.A., Milligan S.R., Coen C.W. & O’Byrne K.T. 2013. Phytoestrogens and gonadotropin-releasing hormone pulse generator activity and pituitary luteinizing hormone release in the rat. Endocrinology 142: 1202-1208.10.1210/endo.142.3.8015Search in Google Scholar PubMed
McKay D.L. & Blumberg J.B. 2007. A review of the bioactivity of South African teas: rooibos (Aspalathus linearis) and honeybush (Cyclopia intermedia). Phytother. Res. 21: 1-16.10.1002/ptr.1992Search in Google Scholar PubMed
Na H.K., Mossanda K.S., Lee J.Y. & Surh Y.J. 2004. Inhibition of phorbol ester-induced COX-2 expression by some edible African plants. Biofactors 2: 149-153.10.1002/biof.552210130Search in Google Scholar PubMed
Nakano M., Ioth Y., Mizuno T. & Nakashima H. 1997. Polysaccharide from Aspalathus linearis with strong anti-HIV activity. Biosci. Biotechnol. Biochem. 61: 71-267.10.1271/bbb.61.267Search in Google Scholar PubMed
Nejaty H., Lacey M. & Whitehead S.A. 2001. Differing effects of endocrine-disrupting chemicals on basal and FSH-stimulated progesterone production in rat granulosa-luteal cells. Exp. Biol. Med. 226: 570-576.10.1177/153537020122600610Search in Google Scholar PubMed
Nikolova V., Petrova S., Petkova V., Pavlova A. & Georgieva T. 2007. Antioxidant effects of rooibos tea on workers occupationally exposed to lead. Toxicol. Lett. 172 (Suppl.): 120-121.10.1016/j.toxlet.2007.05.320Search in Google Scholar
Opuwari C.S. 2013. Effect of tea and herbal infusions on mammalian reproduction and fertility. PhD Thesis, Faculty of Science University of Western Cape, South Africa; http://etd.uwc.ac.za/xmlui/handle/11394/3015.Search in Google Scholar
Opuwari C.S. & Monsees T.K. 2015. Reduced testosterone production in TM3 Leydig cells treated with Aspalathus linearis (rooibos) or Camellia sinensis (tea). Andrologia 47: 52-58.10.1111/and.12221Search in Google Scholar PubMed
Roy S. & Nicholson D.W. 2000. Cross-talk in cell death signaling. J. Exp. Med. 8: 21-25.10.1084/jem.192.8.F21Search in Google Scholar
Schams D., Berishaa B., Kosmanna M., Einspaniera R. & Amselgruberg W.M. 1999. Possible role of growth hormone (IGFs), and IGF-binding proteins in the regulation of ovarian function in large farm animal. Domest. Anim. Endocrinol. 17: 279-285.Search in Google Scholar
Sirotkin A.V. 2014. Regulators of Ovarian Functions. New York, Nova Science Publishers, Inc., 194 pp.Search in Google Scholar
Sirotkin A.V., Florkovičova I., Makarevich A.V., Schaeffer H.J., Kotwica J., Marnet P.G. & Sanislo P. 2003. Oxytocin mediates some effects of insulin-like growth factor-I on porcine ovarian follicles. J. Reprod. Dev. 49: 141-149.10.1262/jrd.49.141Search in Google Scholar PubMed
Sirotkin A.V. & Harrath A.H. 2014. Phytoestrogens and their effects. Eur. J. Pharmacol. 741: 230-236.10.1016/j.ejphar.2014.07.057Search in Google Scholar PubMed
Wang C. & Roy S.K. 2009. Expression of bone morphogenetic protein receptor (BMPR) during perinatal ovary development and primordial folicle formation in the hamster: possible regulation by FSH. Endocrinology 150: 1886-1896.10.1210/en.2008-0900Search in Google Scholar PubMed PubMed Central
Whitehead S.A. & Lacey M. 2003. Phytoestrogens inhibit aromatase but not 17β-hydroxysteroid dehydrogenase (HSD) type 1 in human granulosa-luteal cells: evidence for FSH induction of 17β-HSD. Hum. Reprod. 18: 487-494.10.1093/humrep/deg125Search in Google Scholar PubMed
Yoshikawa T., Naito Y., Oyamada H., Ueda S., Tanigawa T., Takemura T., Sugino S. & Kondo M. 1990. Scavenging effects of Aspalanthus linearis (Rooibos tea) on active oxygen species. Adv. Exp. Med. Biol. 264: 171-174. 10.1007/978-1-4684-5730-8_26Search in Google Scholar PubMed
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Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
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- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
Articles in the same Issue
- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
- Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
- A new species of the genus Aeolothrips (Thysanoptera: Aeolothripidae) from Iran
- Skull variability of mice and voles inhabiting the territory of a great cormorant colony
- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
