Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
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Tatjana I. Vujović
,
Đurđina V. Ružić
Abstract
In vitro shoot tips of Serbian autochthonous plums ‘Sitnica’ (Prunus domestica L.) and ‘Crvena Ranka’ (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum ‘Sitnica’, with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. ‘Crvena Ranka’ exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3- 81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.
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- An overview of phytochrome: An important light switch and photo-sensory antenna for regulation of vital functioning of plants
- A novel GH13 subfamily of α-amylases with a pair of tryptophans in the helix α3 of the catalytic TIM-barrel, the LPDlx signature in the conserved sequence region V and a conserved aromatic motif at the C-terminus
- Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
- Understanding taxonomic position of local endemic Agropyron deweyi (Poaceae) using morphological characters and sequences of nuclear and chloroplast DNA regions
- Genetic status of the putative hybrid swarms of mountain dwarf pine and Scots pine in contact zones of their distribution in Slovakia
- Species delimitation and population structure in three Onosma (Boraginaceae) species
- Glycinebetaine priming improves salt tolerance of wheat
- The effect of nitrogen level on rice growth, carbon-nitrogen metabolism and gene expression
- High efficiency Agrobacterium-mediated transformation of Pinellia ternata using petiole explants from submerged cultures
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- Response of green hydra (Hydra viridissima) to variability and directional changes in food availability
- Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran
- New and firstly recorded oribatid mites from Turkey
- Reproductive parameters of four species of water mites (Acari: Hydrachnidia)
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- High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner
- Direct influence of rooibos-derived compound on rabbit ovarian functions and their response to gonadotropins
- Corrective notice to the European mudminnow (Umbra krameri Walbaum, 1792) record from the Black Sea
