A Micellar-Enhanced Spectrofluorimetric Method for the Determination of Ciprofloxacin in Pure Form, Pharmaceutical Preparations and Biological Samples

Muhammad Naeem Khan; Niaz Bibi; Irum; Muhammad Idrees

doi:10.3139/113.110646

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A Micellar-Enhanced Spectrofluorimetric Method for the Determination of Ciprofloxacin in Pure Form, Pharmaceutical Preparations and Biological Samples

Muhammad Naeem Khan , Niaz Bibi , Irum und Muhammad Idrees

Veröffentlicht/Copyright: 13. November 2019

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Aus der Zeitschrift Tenside Surfactants Detergents Band 56 Heft 6

Abstract

A simple, fast and interference free spectrofluorimetric method was developed for the quantification of ciprofloxacin in its pure form, pharmaceutical preparations and in human plasma. The method is based on the enhancement of the weak fluorescence signal of drugs by the addition of sodium dodecyl sulphate (SDS) surfactant micelles in citrate buffer at pH 5.5. Different experimental parameters like buffers, surfactant type and concentration were investigated. The fluorescence intensity of ciprofloxacin was measured at 446 nm (emission) and at 328 nm (excitation). A linear relationship between concentration of ciprofloxacin and fluorescence intensity was found in the range of 0.04 μg/mL–6.0 μg/mL with a correlation coefficient of 0.9946. The limit of detection (LOD) and limit of quantification (LOQ) were calculated and found to be 3.87 × 10⁻⁵ and 1.29 × 10⁻⁴ μg/mL respectively. The effect of common excipients and co-administered drugs were also investigated and the method was found free of interferences. The method was successfully applied for the determination of ciprofloxacin in pharmaceutical preparations and human plasma with percentage recoveries of 97.08 % to 99.32 % and 95.00 % to 101.25 % respectively.

Kurzfassung

Zur Quantifizierung von Ciprofloxacin in reiner Form, in pharmazeutischen Präparaten und in Humanplasma wurde eine einfache, schnelle und störungsfreie spektrofluorimetrische Methode entwickelt. Das Verfahren basiert auf der Verstärkung des schwachen Fluoreszenzsignals von Arzneiwirkstoffen durch Zugabe von Natriumdodecylsulfatmizellen (SDS-Mizellen) in Citratpuffer bei pH 5,5. Verschiedene experimentelle Parameter wie Puffer, Tensidtyp und Konzentration wurden untersucht. Die Fluoreszenzintensität von Ciprofloxacin wurde bei 328 nm (Anregung) und die Emission bei 446 nm (Emission) gemessen. Ein linearer Zusammenhang zwischen der Ciprofloxacinkonzentration und der Fluoreszenzintensität mit einem Korrelationskoeffizienten von 0,9946 wurde im Bereich von 0,04 μg/mL–6,0 μg/mL gefunden. Die Nachweisgrenze (LOD) und die Bestimmungsgrenze (LOQ) wurden berechnet als 3,87 × 10⁻⁵ μg/mL bzw. 1,29 × 10⁻⁴ μg/mL. Die Wirkung gebräuchlicher Hilfsstoffe und gleichzeitig verabreichter Arzneiwirkstoffe wurde ebenfalls untersucht. Die Untersuchungsmethode erwies sich als störungsfrei. Mit der Methode wurde Ciprofloxacin in pharmazeutischen Präparaten und Humanplasma erfolgreich bestimmt. Die prozentualen Wiederfindungsraten lagen zwischen 97,08 % bis 99,32 % bzw. 95,00 % bis 101,25 %.

Key words: Ciprofloxacin; sodium dodecyl sulfate; micellar-enhanced spectrofluorimetry; pharmaceutical preparations; human plasma

Stichwörter: Ciprofloxacin; Natriumdodecylsulfat; durch Mizellen verstärkte Spektrofluorimetrie; pharmazeutische Präparate; Humanplasma

∗Correspondence address, Dr. Muhammad Naeem Khan, Department of Chemistry, Bacha Khan University, 24420 Charsadda, Pakistan, Fax: 92 – 91 – 6540060, E-Mail: mnaeemchemist@yahoo.com

Dr. Muhammad Naeem Khan did his PhD in Analytical Chemistry in 2013 from the Institute of Chemical Sciences University of Peshawar under the supervision of Prof. Dr. Muhammad Rasul Jan (Professor Emeritus) and Professor Dr. Jasmin Shah (Meritorious Professor). Dr. Muhamamd Naeem Khan is currently working as Assistant Professor of Chemistry in the Department of Chemistry Bacha Khan University Charsadda. Dr. Khan is supervising a number of MPhil, MSc and BS research students. He has published 17 papers in international journals. He is doing research on method development and has the skill to work on UV/Visible spectrophotometer, Fluorometer, HPLC, Atomic Absorption Spectroscopy and GC.

Ms Niaz Bibi has completed her MSc in analytical Chemistry. She did research in MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is from her research work which she carried out along with her classmate Ms Irum. Ms. Niaz Bib has passed all the courses and she stands first in the class obtaining 4.0 CGPA out of 4.0. She is one of the talented students of the department. She was taking keen interest in her research work.

Ms Irum has completed her MSc in analytical Chemistry. She did research in MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is from her research work which she carried out along with her classmate Ms Niaz Bibi. Ms. Irum has passed all the courses with good grade. She is also one of the talented students of the department. She was taking keen interest in her research work and did her research along with Ms. Niaz Bibi.

Dr. Muhammad Idrees did his PhD in Analytical Chemistry in 2013 from Brazil. He did his PhD research work in Analytical Chemistry and has published a number of papers. Dr. Muhamamd Idrees is currently working as Assistant Professor of Chemistry in the Department of Chemistry Bacha Khan University Charsadda since 2013. Dr. Idrees is supervising a number of MPhil, MSc and BS research students. He has published 13 papers in international journals. He is doing research on method development and metals determiantion and has the skill to work on UV/Visible spectrophotometer, Fluorometer, HPLC, Atomic Absorption Spectroscopy and GC.

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Received: 2018-09-21

Accepted: 2019-03-13

Published Online: 2019-11-13

Published in Print: 2019-11-15

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Artikel in diesem Heft

https://doi.org/10.3139/113.110646

Schlagwörter für diesen Artikel

Ciprofloxacin; sodium dodecyl sulfate; micellar-enhanced spectrofluorimetry; pharmaceutical preparations; human plasma