Reduction of nitroblue tetrazolium to formazan by folic acid
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Cesare Achilli
,
Stefania Grandi
Abstract
The reduction of nitroblue tetrazolium (NBT) to formazan by folic acid, N-(4-aminobenzoyl) glutamic acid, and other amino acids was studied in this paper. The reduction involves only one of the two tetrazolium rings of NBT. The reaction is considerably more rapid with folic acid and N-(4-aminobenzoyl) glutamic acid than with the other amino acids under study. The electron donor moiety appears to be the carboxylic acid in the alpha position. N-ethyl-N′(3-dimethylaminopropyl) carbodiimide notably increases the rate of the reaction and promotes the reduction of both tetrazolium rings.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- A spectrophotometric method for plant pigments determination and herbs classification
- Catalysis and reaction mechanisms of N-formylation of amines using Fe(III)-exchanged sepiolite
- Effect of support on activity of palladium catalysts in nitrobenzene hydrogenation
- Biphasic recognition chiral extraction — novel way of separating pantoprazole enantiomers
- Effect of the preparation route on the structure and microstructure of LaCoO3
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- Mercury(II) complexes of new bidentate phosphorus ylides: synthesis, spectra and crystal structures
- Synthesis and properties of CaAl-layered double hydroxides of hydrocalumite-type
- MgZnAl hydrotalcite-like compounds preparation by a green method: effect of zinc content
- Carbon nanotube-layered double hydroxide nanocomposites
- Synthesis of palladium-bidentate complex and its application in Sonogashira and Suzuki coupling reactions
- Reduction of nitroblue tetrazolium to formazan by folic acid
- Michael addition of phenylacetonitrile to the acrylonitrile group leading to diphenylpentanedinitrile. Structural data and theoretical calculations
- Efficient hydrolysis of glucose-1-phosphate catalyzed by metallomicelles with histidine residue
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