Abstract
Twenty herbs, showing medicinal benefits on human life, like Taraxacum officinale, Plantago lanceolata, Hypericum perforatum, Ocimum basilicum, Melissa officinalis, Mentha piperita, and others, were tested. The percentage content of pigments, i.e. chlorophylls, carotenoids, and flavonoids, in the chosen herbs was determined. The spectrophotometric method, which is quick and readily available, was used. Measurements required pigment extraction with a solvent. Ethanol was used for the determination of chlorophylls and carotenoids, and acetone for the determination of flavonoids. Hierarchical agglomerative cluster analysis was performed in order to confirm the experimental results. The study of flavonoids content showed an interesting regularity which can be used for the classification of herbs. Plants belonging to the Lamiaceae family showed the flavonoids content in the range from 0.18 % to 0.68 %, whereas those belonging to the Apiaceaehas family showed the flavonoids content in the 0.75–1.20 % range.
[1] Bianchi, T. S., Demetropoulos, A., Hadjichristophorou, M., Argyrou, M., Baskaran, M., & Lambert, C. (1996). Plant pigments as biomarkers of organic matter sources in sediments and coastal waters of Cyprus (eastern Mediterranean). Estuarine, Coastal and Shelf Science, 42, 103–115. DOI: 10.1006/ecss.1996.0008. http://dx.doi.org/10.1006/ecss.1996.000810.1006/ecss.1996.0008Search in Google Scholar
[2] Bianchi, T. S., & Canuel, E. A. (2011). Chemical biomarkers in aquatic ecosystems. Princeton, NJ, USA: Princeton University Press. Search in Google Scholar
[3] Biehler, E., Mayer, F., Hoffmann, L., Krause, E., & Bohn, T. (2010). Comparison of 3 spectrophotometric methods for carotenoid determination in frequently consumed fruits and vegetables. Journal of Food Science, 75, C55–C61. DOI: 10.1111/j.1750-3841.2009.01417.x. http://dx.doi.org/10.1111/j.1750-3841.2009.01417.x10.1111/j.1750-3841.2009.01417.xSearch in Google Scholar
[4] Boo, H. O., Hwang, S. J., Bae, C. S., Park, S. H., Heo, B. G., & Gorinstein, S. (2012). Extraction and characterization of some natural plant pigments. Industrial Crops and Products, 40, 129–135. DOI: 10.1016/j.indcrop.2012.02.042. http://dx.doi.org/10.1016/j.indcrop.2012.02.04210.1016/j.indcrop.2012.02.042Search in Google Scholar
[5] Capecka, E., Mareczek, A., & Leja, M. (2005). Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93, 223–226. DOI: 10.1016/j.foodchem.2004.09.020. http://dx.doi.org/10.1016/j.foodchem.2004.09.02010.1016/j.foodchem.2004.09.020Search in Google Scholar
[6] Dudek, G., Grzywna, Z. J., & Willcox, M. L. (2008). Classification of antituberculosis herbs for remedial purposes by using fuzzy sets. Biosystems, 94, 285–289. DOI: 10.1016/j.biosystems.2008.05.038. http://dx.doi.org/10.1016/j.biosystems.2008.05.03810.1016/j.biosystems.2008.05.038Search in Google Scholar
[7] Grzywna, Z. J., Borys, P., & Dudek, G. (2006). On the mathematical reconstruction of two dimensional plants. Bioscience Reports, 26, 113–129. DOI: 10.1007/s10540-006-9011-2. http://dx.doi.org/10.1007/s10540-006-9011-210.1007/s10540-006-9011-2Search in Google Scholar
[8] Kartashova, G. S., & Sudos, L. V. (1997). Quantitative determination of flavonoids in the above-ground part of agrimony plants. Pharmaceutical Chemistry Journal, 31, 488–491. DOI: 10.1007/bf02464310. http://dx.doi.org/10.1007/BF0246431010.1007/BF02464310Search in Google Scholar
[9] Klein, B., & Sournia, A. (1987). A daily study of the diatom spring bloom at Roscoff (France) in 1985. II. Phytoplankton pigment composition studied by HPLC analysis. Marine Ecology — Progress Series, 37, 265–275. http://dx.doi.org/10.3354/meps03726510.3354/meps037265Search in Google Scholar
[10] Kurtyka, R., Małkowski, E., Burdach, Z., Kita, A., & Karcz, W. (2012). Interactive effects of temperature and heavy metals (Cd, Pb) on the elongation growth in maize coleoptiles. Computes Rendus Biologies, 335, 292–299. DOI: 10.1016/j.crvi.2012.03.012. http://dx.doi.org/10.1016/j.crvi.2012.03.01210.1016/j.crvi.2012.03.012Search in Google Scholar
[11] Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148C, 350–382. DOI: 10.1016/0076-6879(87)48036-1. http://dx.doi.org/10.1016/0076-6879(87)48036-110.1016/0076-6879(87)48036-1Search in Google Scholar
[12] Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and carotenoids: measurement and characterization by UVVIS spectroscopy. In R. E. Wrolstad (Ed.), Current protocols in food analytical chemistry (pp. F4–F4.3.8). New York, NY, USA: Wiley. Search in Google Scholar
[13] Marr, I. L., Suryana, N., Lukulay, P., & Marr, M. I. (1995). Determination of chlorophyll a and b by simultaneous multicomponent spectrophotometry. Fresenius’ Journal of Analytical Chemistry, 352, 456–460. DOI: 10.1007/bf00323366. http://dx.doi.org/10.1007/BF0032336610.1007/BF00323366Search in Google Scholar
[14] Massart, D. L., & Kauffman, L. (1983). The interpretation of analytical data by use of cluster analysis. New York, NY, USA: Wiley. Search in Google Scholar
[15] Milde, D., Machaček, J., & StuŽka, V. (2007). Evaluation of colon cancer elements contents in serum using statistical methods. Chemical Papers, 61, 348–352. DOI: 10.2478/s11696-007-0046-z. http://dx.doi.org/10.2478/s11696-007-0046-z10.2478/s11696-007-0046-zSearch in Google Scholar
[16] Pessarakli, M. (2010). Handbook of plant and crop stress. Boca Raton, FL, USA: CRC Press. http://dx.doi.org/10.1201/b1032910.1201/b10329Search in Google Scholar
[17] Plante, R., Plante-Cuny, M. R., & Reys, J. R. (1986). Photosynthetic pigments of sandy sediments on the north Mediterranean coast: their spatial distribution and its effect on sampling strategies. Marine Ecology — Progress Series, 34, 133–141. http://dx.doi.org/10.3354/meps03413310.3354/meps034133Search in Google Scholar
[18] Polish Pharmaceutical Society (2002). Farmakopea Polska VI. Warsaw, Poland: PZWL. (in Polish) Search in Google Scholar
[19] Schoefs, B. (2000). The pigment composition of pumpkin seed oil. In J. Francis (Ed.), Proceedings of the 4th International Symposium on Natural Colourants (pp. 85–92). London, UK: SCI Publishers. Search in Google Scholar
[20] Schoefs, B. (2002). Chlorophyll and carotenoid analysis in food products. Properties of the pigments and methods of analysis. Trends in Food Science & Technology, 13, 361–371. DOI: 10.1016/s0924-2244(02)00182-6. http://dx.doi.org/10.1016/S0924-2244(02)00182-610.1016/S0924-2244(02)00182-6Search in Google Scholar
[21] Soares, L. A. L., Bassani, V. L., Gonzalez Ortega, G., & Petrovick, P. R. (2003). Total flavonoid determination for the quality control of aqueous extractives from Phyllanthus niruri L. Latin American Journal of Pharmacy, 22, 203–207. Search in Google Scholar
[22] Strzelecka, H., Kamińska, J., Kowalski, J., & Walewska, E. (1982). Chemiczne metody badań roślinnych surowców leczniczych. Warsaw, Poland: PZWL. (in Polish) Search in Google Scholar
[23] Sun, X. H., Yang, X. R., & Wang, E. K. (2005). Chromatographic and electrophoretic procedures for analyzing plant pigments of pharmacologically interests. Analytica Chimica Acta, 547, 153157. DOI: 10.1016/j.aca.2005i.05.051. http://dx.doi.org/10.1016/j.aca.2005.05.051Search in Google Scholar
[24] Ustin, S. L., Gitelson, A. A., Jacquemoud, S., Schaepman, M., Asner, G. P., Gamon, J. A., & Zarco-Tejada, P. (2009). Retrieval of foliar information about plant pigment systems from high resolution spectroscopy. Remote Sensing of Environment, 113, S67–S77. DOI: 10.1016/j.rse.2008.10.019. http://dx.doi.org/10.1016/j.rse.2008.10.01910.1016/j.rse.2008.10.019Search in Google Scholar
[25] Vaulot, D., Partensky, F., Neveux, J., Mantoura, R. F. C., & Llewellyn, C. A. (1990). Winter presence of prochlorophytes in surface waters of the northwestern Mediterranean Sea. Limnology and Oceanography, 35, 1156–1164. http://dx.doi.org/10.4319/lo.1990.35.5.115610.4319/lo.1990.35.5.1156Search in Google Scholar
© 2013 Institute of Chemistry, Slovak Academy of Sciences
Articles in the same Issue
- A spectrophotometric method for plant pigments determination and herbs classification
- Catalysis and reaction mechanisms of N-formylation of amines using Fe(III)-exchanged sepiolite
- Effect of support on activity of palladium catalysts in nitrobenzene hydrogenation
- Biphasic recognition chiral extraction — novel way of separating pantoprazole enantiomers
- Effect of the preparation route on the structure and microstructure of LaCoO3
- Synthesis, characterisation, and antioxidant study of Cr(III)-rutin complex
- Mercury(II) complexes of new bidentate phosphorus ylides: synthesis, spectra and crystal structures
- Synthesis and properties of CaAl-layered double hydroxides of hydrocalumite-type
- MgZnAl hydrotalcite-like compounds preparation by a green method: effect of zinc content
- Carbon nanotube-layered double hydroxide nanocomposites
- Synthesis of palladium-bidentate complex and its application in Sonogashira and Suzuki coupling reactions
- Reduction of nitroblue tetrazolium to formazan by folic acid
- Michael addition of phenylacetonitrile to the acrylonitrile group leading to diphenylpentanedinitrile. Structural data and theoretical calculations
- Efficient hydrolysis of glucose-1-phosphate catalyzed by metallomicelles with histidine residue
- Synthesis of [Re2Cl4(O)2(µ-O)(3,5-lut)4] and investigation of its structure via X-ray and spectroscopic measurements and DFT calculations
- QSAR modeling of aromatase inhibition by flavonoids using machine learning approaches
- Influence of freezing on physicochemical forms of natural and technogenic radionuclides in Chernozem soil
- “Green synthesis” of benzothiazepine library of indeno analogues and their in vitro antimicrobial activity
Articles in the same Issue
- A spectrophotometric method for plant pigments determination and herbs classification
- Catalysis and reaction mechanisms of N-formylation of amines using Fe(III)-exchanged sepiolite
- Effect of support on activity of palladium catalysts in nitrobenzene hydrogenation
- Biphasic recognition chiral extraction — novel way of separating pantoprazole enantiomers
- Effect of the preparation route on the structure and microstructure of LaCoO3
- Synthesis, characterisation, and antioxidant study of Cr(III)-rutin complex
- Mercury(II) complexes of new bidentate phosphorus ylides: synthesis, spectra and crystal structures
- Synthesis and properties of CaAl-layered double hydroxides of hydrocalumite-type
- MgZnAl hydrotalcite-like compounds preparation by a green method: effect of zinc content
- Carbon nanotube-layered double hydroxide nanocomposites
- Synthesis of palladium-bidentate complex and its application in Sonogashira and Suzuki coupling reactions
- Reduction of nitroblue tetrazolium to formazan by folic acid
- Michael addition of phenylacetonitrile to the acrylonitrile group leading to diphenylpentanedinitrile. Structural data and theoretical calculations
- Efficient hydrolysis of glucose-1-phosphate catalyzed by metallomicelles with histidine residue
- Synthesis of [Re2Cl4(O)2(µ-O)(3,5-lut)4] and investigation of its structure via X-ray and spectroscopic measurements and DFT calculations
- QSAR modeling of aromatase inhibition by flavonoids using machine learning approaches
- Influence of freezing on physicochemical forms of natural and technogenic radionuclides in Chernozem soil
- “Green synthesis” of benzothiazepine library of indeno analogues and their in vitro antimicrobial activity