Ocimum basilicum alleviates blood glucose, lipid profile and iNOS in diabetes gestational rat model
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Hirowati Ali
,
Hasmiwati
Abstract
Objectives
Gestational diabetes (GDM) complications affect maternal and fetus in utero. GDM’s vascular dysfunction showed inducible nitric oxide synthase (iNOS) alteration and was linked to the higher production of nitrogen species, leading to diabetic embryopathy. Ocimum basilicum (O. basilicum) has been reported for its anti-inflammatory and anti-diabetic effects. Thus, the present study investigates the anti-diabetic effect, lipid-lowering effect, and iNOS expression in GDM animal models treated with O. basilicum extract.
Experimental procedures
Four groups of pregnant rats consist of control and GDM groups. One GDM group was set for control positive. Two GDM groups were treated with O. basilicum extract in two doses (100 and 200 mg/kg BW) for 14 days. Blood glucose of all groups was observed at 72 h after STZ injection and 14 days after administration of O. basilicum extract. Lipid profile and iNOS expression using real-time PCR were measured afterward.
Results
O. basilicum extract lowered blood glucose levels in both doses, from 262.60 mg/dL±6.89–136.80 mg/dL ± 15.6 mg/dL and 113.20 mg/dL±5.25 mg/dL. Total cholesterol, LDL and triglyceride showed a reduction, especially in 200 mg/kg BW dose extract from 122.37 mg/dL ± 14.84 mg/dL, 69.75 mg/dL±3.78 mg/dL and 137.51 mg/dL ± 8.12–74.64 mg/dL±8.71 mg/dL, 40.26 mg/dL±3.31 mg/dL and 87.57 mg/dL±6.29 mg/dL, respectively. iNOS expression downregulated in both doses, from 2.17±0.39 to 0.94±0.3 and 0.41±0.08.
Conclusions
This study showed that O. basilicum extract has a potential therapeutic activity in lowering blood glucose, improved lipid profile, and downregulating iNOS in GDM.
Acknowledgments
The authors would like to thank to DH and ED for critical suggestion for this research.
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Research funding: This study was supported by The Biomedical Laboratory, Faculty of Medicine, Andalas University, Indonesia.
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Author contributions: Study conception or design: HA, H, RR, DH, EU, EY, ED; Data analyzing and draft manuscript preparation: HA; Critical revision of the paper: HA, VB, E, EY, ED; Supervision of the research: HA, DH; Final approval of the version to be published: HA, H, RR, DH, EU, EY, ED.
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Competing interests: Authors state no conflict of interest.
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Ethical approval: Ethical approval for animal study approved by The Committee of The Research Ethics of The Faculty of Medicine, Andalas University No. 378/KEP/FK/2019.
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- Frontmatter
- Reviews
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- Phytochemical: a treatment option for heavy metal induced neurotoxicity
- The effects of curcumin-containing supplementations on inflammatory markers and lipid profiles in patients with chronic kidney diseases: a systematic review and meta-analysis of randomized controlled trials
- A systematic review on traditional medicinal Animal’s parts and products used for the treatment of respiratory tract disorders in Ethiopia
- Review on nutraceuticals: phase transition from preventive to protective care
- A study of effect of Centella asiatica on oxidative markers in the hippocampus of offsprings born to alcohol-fed pregnant rats and the correlation with their cognitive functions
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