Home Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats
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Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats

  • Gerard Bessan Dossou-Agoin EMAIL logo , Adam Gbankoto , Simon Azonbakin , Razack Osseni , Achille Yemoa and Anatole Lalèyè
Published/Copyright: January 10, 2022
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 3

Abstract

Objectives

Lead exposure seriously impairs male reproductive function. The protective capacity of Pedalium murex leafy stem and fruit aqueous extracts against lead testicular toxicity is evaluated to find herbals drugs able to improve semen quality.

Methods

Phytochemical screening were performed according to classical methods. Twenty four male rats were divided into four groups of six rats each and received the following treatments via oral route: distilled water; 0.2% lead acetate in drinking water; 0.2% lead acetate in drinking water with 400 mg/kg P. murex aqueous leafy stem extract; 0.2% leaded water with 400 mg/kg P. murex aqueous fruit extract. Treatments were administered for 70 days. Body and reproductive organs weights, sperm parameters and testicular histological sections of each group were examined.

Results

Flavonoids, tannins, coumarins, alkaloids, and lignans were found in both extracts. Lead intoxication reduced sperm motility and count but increased the percentage of morphologically abnormal sperms. The germinal epithelium of seminiferous tubules histoarchitecture was disorganized by lead. The leafy stem extract was effective in reducing lead induced testicular disruption whereas fruit has not shown any beneficial effect.

Conclusions

P. murex leafy stem aqueous extract is effective against semen alterations caused by lead.

Keywords: lead; leafy stem; male infertility; Pedalium murex ; testicular toxicity
Corresponding author: Gerard Bessan Dossou-Agoin, Experimental Physiology and Pharmacology Laboratory, Faculty of Sciences and Technology, University of Abomey-Calavi, Cotonou Benin, Benin, E-mail:

Acknowledgment

Authors want to thank Honoré da-Zocanclounon for its invaluable help in the histological analysis.

  1. Research funding: None declared.

  2. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  3. Competing interest: The funding organization(s) played no role in the study design, in the collection, analysis and interpretation of data; or in the decision to submit the report for publication.

  4. Ethical approval: The research related to the animals use has been complied with all the relevant national regulations and institutional policies for the care and use of animals.

References

1. Carlsen, E, Giwercman, A, Keiding, N, Skakkebaek, NE. Evidence for decreasing quality of semen during past 50 years. Obstet Gynecol Surv 1992;48:200–2. https://doi.org/10.1136/bmj.305.6854.609.Search in Google Scholar PubMed PubMed Central

2. Rahban, R, Priskorn, L, Senn, A, Stettler, E, Galli, F, Vargas, J, et al.. Semen quality of young men in Switzerland: a nationwide cross‐sectional population‐based study. Andrology 2019;7:1–9. https://doi.org/10.1111/andr.12645.Search in Google Scholar PubMed PubMed Central

3. Bae, WJ, Ha, US, Choi, JB, Kim, KS, Kim, SJ, Cho, HJ, et al.. Protective effect of decursin extracted from Angelica gigas in male infertility via Nrf2/HO-1 signaling pathway. Oxid Med Cell Longev 2016;2016:1–9. https://doi.org/10.1155/2016/5901098.Search in Google Scholar PubMed PubMed Central

4. Vigeh, M, Smith, DR, Hsu, PC. How does lead induce male infertility? Iran J Reprod Med 2011;9:1–8.Search in Google Scholar

5. Jegede, AI, Offor, U, Azu, OO, Akinloye, O. Red palm oil attenuates lead acetate induced testicular damage in adult male Sprague-Dawley rats. Evid base Compl Alternative Med 2015;2015:1–7. https://doi.org/10.1155/2015/130261.Search in Google Scholar PubMed PubMed Central

6. Gandhi, J, Hernandez, RJ, Chen, A, Smith, NL, Sheynkin, YR, Joshi, G, et al.. Impaired hypothalamic-pituitary-testicular axis activity, spermatogenesis, and sperm function promote infertility in males with lead poisoning. Zygote 2017;25:103–10. https://doi.org/10.1017/s0967199417000028.Search in Google Scholar PubMed

7. Elgawish, RAR, Abdelrazek, HMA. Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicol Rep 2014;1:795–801. https://doi.org/10.1016/j.toxrep.2014.10.010.Search in Google Scholar PubMed PubMed Central

8. Devanesan, AA, Zipora, T, Bell, BA, Deviram, G, Thilagar, S. Phytochemical and pharmacological status of indigenous medicinal plant Pedalium murex L.—a review. Biomed Pharmacother 2018;103:1456–63. https://doi.org/10.1016/j.biopha.2018.04.177.Search in Google Scholar PubMed

9. The National Academies Press, editor. Commitee for the update of the guide for the care and use of laboratory animals. Guide for the care and use of laboratory animals, 8th ed. Washington: National Research Council; 2011:1–220 pp.Search in Google Scholar

10. El-Magd, MA, Kahilo, KA, Nasr, NE, Kamal, T, Shukry, M, Saleh, AA. A potential mechanism associated with lead-induced testicular toxicity in rats. Andrologia 2017;49:1–9. https://doi.org/10.1111/and.12750.Search in Google Scholar PubMed

11. Wagner, H, Bladt, S. Plant drug analysis: a thin layer chromatography atlas, 2nd ed. Berlin, Heidelberg, New York: Springer-Verlag. B: Springer-Verlag; 1996:368 p.10.1007/978-3-642-00574-9Search in Google Scholar

12. Iqbal, E, Abu, K, Lim, LBL. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J King Saud Univ Sci 2015;27:224–32. https://doi.org/10.1016/j.jksus.2015.02.003.Search in Google Scholar

13. Balamurugan, G, Muralidharan, P, Polapala, S. Aphrodisiac activity and curative effects of Pedalium murex (L.) against ethanol-induced infertility in male rats. Turk J Biol 2010;34:153–63.10.3906/biy-0808-8Search in Google Scholar

14. Hayes, AW, Kruger, CL, editors. Principles and methods of toxicology, 6th ed. New York, NY 10017: CRC Press; 2014:2184 p.10.1201/b17359Search in Google Scholar

15. Nguimatio, K, Xavier, F, Defo, D, Brice, P, Modeste, WN, Pierre, W, et al.. Preventive effects of Aframomum melegueta extracts on the reproductive complications of propylthiouracil – induced hypothyroidism in male rat. Andrologia 2019;51:1–12.10.1111/and.13306Search in Google Scholar

16. Seed, J, Chapin, RE, Clegg, ED, Dostal, LA, Foote, RH, Hurtt, ME, et al.. Methods for assessing sperm motility, morphology and counts in the rat, rabbit and dog: a consensus report. ILSI Risk Science Institute Expert Working Group on Sperm Evaluation. Reprod Toxicol 1996;10:237–44.10.1016/0890-6238(96)00028-7Search in Google Scholar

17. Wang, Y. Epididymal sperm count. Curr Protoc Toxicol 2002;14:1–5. https://doi.org/10.1002/0471140856.tx1606s14.Search in Google Scholar PubMed

18. Shafai, AE, Zohdy, N, Mulla, KE, Hassan, M, Morad, N. Light and electron microscopic study of the toxic effect of prolonged lead exposure on the seminiferous tubules of albino rats and the possible protective effect of ascorbic acid. Food Chem Toxicol 2011;49:734–43. https://doi.org/10.1016/j.fct.2010.11.033.Search in Google Scholar PubMed

19. Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for lead. Atlanta, Georgia: US Department for Health and Human Services; 2019.Search in Google Scholar

20. Assi, MA, Hezmee, MNM, Haron, AW, Sabri, MYM, Rajion, MA. The detrimental effects of lead on human and animal health. Vet World 2016;9:660–71. https://doi.org/10.14202/vetworld.2016.660-671.Search in Google Scholar PubMed PubMed Central

21. Offor, SJ, Mbwagu, HOC, Orisakwe, OE. Lack of beneficial effect of activated charcoal in lead induced testicular toxicity in male albino rats. Middle East Fertil Soc J 2017;22:189–92. https://doi.org/10.1016/j.mefs.2017.02.001.Search in Google Scholar

22. Wang, C, Zhang, Y, Liang, J, Shan, G, Wang, Y, Shi, Q. Impacts of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in testis. Clin Chim Acta 2006;370:82–8. https://doi.org/10.1016/j.cca.2006.01.023.Search in Google Scholar PubMed

23. US Environmental Protection Agency (EPA). Guidelines for reproductive toxicity risk assessment. Washington, DC, USA: Federal Register; 1996, 61:56274–322 pp.Search in Google Scholar

24. Isaac, JA, Bolanle, AM, Oluyemi, A. Modulatory effects of kolaviron (Garcinia kola extract) on spermogram and reproductive system of adult male wistar rats in lead acetate induced toxiocity. J Toxicol Environ Health Sci 2013;5:121–30. https://doi.org/10.5897/jtehs2013.0262.Search in Google Scholar

25. Dorostghoal, M, Seyyednejad, SM, Noroozi, M, Nejad, T. Cichorium intybus L. extract ameliorates testicular oxidative stress induced by lead acetate in male rats. Clin Exp Reprod Med 2020;47:161–7. https://doi.org/10.5653/cerm.2019.03496.Search in Google Scholar PubMed PubMed Central

26. Li, C, Zhao, K, Zhang, H, Liu, L, Xiong, F, Wang, K, et al.. Lead exposure reduces sperm quality and DNA integrity in mice. Environ Toxicol 2018;33:1–9. https://doi.org/10.1002/tox.22545.Search in Google Scholar

27. Abdrabou, MI, Elleithy, EMM, Yasin, NAE, Shaheen, YM, Galal, M. Ameliorative effects of Spirulina maxima and Allium sativum on lead acetate- induced testicular injury in male albino rats with respect to caspase-3 gene expression. Acta Histochem 2018;121:198–206. https://doi.org/10.1016/j.acthis.2018.12.006.Search in Google Scholar

28. Gurer, H, Ercal, N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000;29:927–45. https://doi.org/10.1016/s0891-5849(00)00413-5.Search in Google Scholar

29. Aitken, RJ, Gibb, Z, Baker, MA, Drevet, J, Gharagozloo, P. Causes and consequences of oxidative stress in spermatozoa. Reprod Fertil Dev 2016;28:1–10. https://doi.org/10.1071/rd15325.Search in Google Scholar PubMed

30. De Lamirande, E, Gagnon, C. Reactive oxygen and human spermatozoa I. Effects on the motility of intact spermatozoa and on sperm axonemes. J Androl 1992;13:368–78.10.1002/j.1939-4640.1992.tb03327.xSearch in Google Scholar

31. Aitken, RJ, Smith, TB, Jobling, MS, Baker, MA, De Iuliis, GN. Oxidative stress and male reproductive health. Asian J Androl 2014;16:31–8. https://doi.org/10.4103/1008-682x.122203.Search in Google Scholar

32. Dossou-agoin, GB, Ganfon, H, Assogba, F, Gbankoto, A, Gbenou, J, Anatole, L. Antioxidant activities of the aqueous extracts of Pedalium murex D. Royen EX L. fruit and leafy stem. Eur J Med Plants 2021;32:1–9. https://doi.org/10.9734/ejmp/2021/v32i830406.Search in Google Scholar

33. Dorostghoal, M, Seyyednejad, SM, Jabari, A. Protective effects of Fumaria parviflora L. on lead-induced testicular toxicity in male rats. Andrologia 2014;46:437–46. https://doi.org/10.1111/and.12100.Search in Google Scholar PubMed

34. Gomathi, S, Sundaram, R, Vijayabaskaran, M, Kannan, C, Sambathkumar, R. Pedalium murex Linn leaves against LPS-induced oxidative stress, anxiety and depression behavioural alterations in rats. Res J Pharm Technol 2017;10:1333–8. https://doi.org/10.5958/0974-360x.2017.00236.0.Search in Google Scholar

35. Huyut, Z, Beydemir, S, Gülçin, I. Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. Biochem Res Int 2017;2017:7616791. https://doi.org/10.1155/2017/7616791.Search in Google Scholar PubMed PubMed Central

36. Pereira, DM, Valentão, P, Pereira, JA, Andrade, PB. Phenolics: from chemistry to biology. Molecules 2009;14:2202–11. https://doi.org/10.3390/molecules14062202.Search in Google Scholar

37. Rao, F, Zhai, Y, Sun, F. Punicalagin mollifies lead acetate-induced oxidative imbalance in male reproductive system. Int J Mol Sci 2016;17:1269. https://doi.org/10.3390/ijms17081269.Search in Google Scholar PubMed PubMed Central

38. Ferramosca, A, Lorenzetti, S, Giacomo, MD, Lunetti, P, Murrieri, F, Capobianco, L, et al.. Modulation of human sperm mitochondrial respiration efficiency by plant polyphenols. Antioxidants 2021;10:1–15. https://doi.org/10.3390/antiox10020217.Search in Google Scholar PubMed PubMed Central

39. Sharma, M, Arya, D, Bhagour, K, Gupta, RS. Modulatory effects of methanolic fruit fraction of Pedalium murex on sulphasalazine-induced male reproductive disruption. Andrologia 2018;51:1–9. https://doi.org/10.1111/and.13190.Search in Google Scholar PubMed

40. Sharma, V, Thakur, M, Dixit, VK. A comparative study of ethanolic extracts of Pedalium murex Linn. fruits and sildenafil citrate on sexual behaviors and serum testosterone level in male rats during and after treatment. J Ethnopharmacol 2012;143:201–6. https://doi.org/10.1016/j.jep.2012.06.024.Search in Google Scholar PubMed

41. Mabrouk, A. Therapeutic effect of thymoquinone against lead-induced testicular histological damage in male Wistar rats. Andrologia 2018;50:1–4. https://doi.org/10.1111/and.13014.Search in Google Scholar PubMed

42. Osseni, R, Adanle, E, Adjagba, M, Awede, B, Hountohotègbè, T, Bigot, A, et al.. Modification of biochemical and haematological parameters during 90-days subchronic toxicity assessment of Carissa edulis in Wistar rats. J Toxicol Environ Health Sci 2017;9:7–13. https://doi.org/10.5897/jtehs2017.0379.Search in Google Scholar
Received: 2021-10-17
Accepted: 2021-12-28
Published Online: 2022-01-10

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Evidence implementation among complementary medicine practitioners: a meta-summary
  4. Phytochemical: a treatment option for heavy metal induced neurotoxicity
  5. The effects of curcumin-containing supplementations on inflammatory markers and lipid profiles in patients with chronic kidney diseases: a systematic review and meta-analysis of randomized controlled trials
  6. A systematic review on traditional medicinal Animal’s parts and products used for the treatment of respiratory tract disorders in Ethiopia
  7. Review on nutraceuticals: phase transition from preventive to protective care
  8. A study of effect of Centella asiatica on oxidative markers in the hippocampus of offsprings born to alcohol-fed pregnant rats and the correlation with their cognitive functions
  9. Anxiogenic and anxiolytic effects of memantine injected into the ventral hippocampus in male stressed mice
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  12. Almond oil restores blood parameters, liver function, blood and liver antioxidants and DNA, and liver histology more efficiently than olive oil in favism
  13. Highly efficient microencapsulation of phytonutrients by fractioned cellulose using biopolymer complexation technology
  14. Ocimum basilicum alleviates blood glucose, lipid profile and iNOS in diabetes gestational rat model
  15. Blood pressure and blood sugar-lowering effects of purified gambir on diabetic hypertensive Wistar Kyoto rats
  16. Assessment of anti-inflammatory and antioxidant activity of quercetin–rutin blend (SophorOx™) – an invitro cell based assay
  17. Subchronic toxicological evaluation of EnXtra™ (standardised extract of Alpinia galanga rhizome) in rats
  18. Ficus glumosa Del. reduces phenylhydrazine-induced hemolytic anaemia and hepatic damage in Wistar rats
  19. Screening of anti-cancerous potential of classical Raudra rasa and modified Raudra rasa modified with hiraka bhasma (nanodiamond) through FTIR & LC-MS analysis
  20. Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats
  21. Intrahippocampal co-administration of nicotine and O-acetyl-L-carnitine prevents the H-89-induced spatial learning deficits in Morris water maze
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  25. GC-MS analysis of curculigo orchiodes and medicinal herbs with cytotoxic, hepatoprotective attributes of ethanolic extract from Indian origin
  26. Biochemical and histopathological evaluation of liver, kidney, and pancreas in normal Sprague Dawley rats administered aqueous extract of beetroot (Beta vulgaris)
  27. The effectiveness of emotional freedom techniques (EFT) on depression of postmenopausal women: a randomized controlled trial
  28. The formulation and efficacy of topical Dorema ammoniacum in treating Melasma: a randomized double-blind, placebo-controlled trial
  29. Impact of inhalation aromatherapy with Damask Rose (Rosa damascena) on stress, anxiety and hemodynamic parameters of patients undergoing coronary angiography: a single blind randomized clinical trial
  30. Hydro-alcoholic extract of Morus nigra reduces fasting blood glucose and HbA1c% in diabetic patients, probably via competitive and allosteric interaction with alpha-glucosidase enzyme; a clinical trial and in silico analysis
  31. Prophylactic management of radiation-induced mucositis using herbal mouthwash in patients with head and neck cancer: an assessor-blinded randomized controlled trial
  32. Implementation science in New Zealand naturopathic practice: a cross-sectional study
  33. Issues of mindfulness implementation for oncology caregivers
  34. Immediate effect of cold mud pack therapy on autonomic variables in primary hypertensive individuals: a randomized trial
  35. Effect of a Persian metabolic diet on the functional dyspepsia symptoms in patients with postprandial distress syndrome: a randomized, double-blind clinical trial
https://doi.org/10.1515/jcim-2021-0482
Keywords for this article
lead; leafy stem; male infertility; Pedalium murex; testicular toxicity

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Evidence implementation among complementary medicine practitioners: a meta-summary
  4. Phytochemical: a treatment option for heavy metal induced neurotoxicity
  5. The effects of curcumin-containing supplementations on inflammatory markers and lipid profiles in patients with chronic kidney diseases: a systematic review and meta-analysis of randomized controlled trials
  6. A systematic review on traditional medicinal Animal’s parts and products used for the treatment of respiratory tract disorders in Ethiopia
  7. Review on nutraceuticals: phase transition from preventive to protective care
  8. A study of effect of Centella asiatica on oxidative markers in the hippocampus of offsprings born to alcohol-fed pregnant rats and the correlation with their cognitive functions
  9. Anxiogenic and anxiolytic effects of memantine injected into the ventral hippocampus in male stressed mice
  10. Research Articles
  11. Comparison of antithyroid effects and hepatic complications of methimazole with catechin and its nanoencapsulation form in adult male rats
  12. Almond oil restores blood parameters, liver function, blood and liver antioxidants and DNA, and liver histology more efficiently than olive oil in favism
  13. Highly efficient microencapsulation of phytonutrients by fractioned cellulose using biopolymer complexation technology
  14. Ocimum basilicum alleviates blood glucose, lipid profile and iNOS in diabetes gestational rat model
  15. Blood pressure and blood sugar-lowering effects of purified gambir on diabetic hypertensive Wistar Kyoto rats
  16. Assessment of anti-inflammatory and antioxidant activity of quercetin–rutin blend (SophorOx™) – an invitro cell based assay
  17. Subchronic toxicological evaluation of EnXtra™ (standardised extract of Alpinia galanga rhizome) in rats
  18. Ficus glumosa Del. reduces phenylhydrazine-induced hemolytic anaemia and hepatic damage in Wistar rats
  19. Screening of anti-cancerous potential of classical Raudra rasa and modified Raudra rasa modified with hiraka bhasma (nanodiamond) through FTIR & LC-MS analysis
  20. Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats
  21. Intrahippocampal co-administration of nicotine and O-acetyl-L-carnitine prevents the H-89-induced spatial learning deficits in Morris water maze
  22. Developmental effects of early-life stress on dopamine D2 receptor and proteins involved in noncanonical D2 dopamine receptor signaling pathway in the prefrontal cortex of male rats
  23. Probiotic administration enhanced antitrypanosomal effects of diminazene aceturate in dogs experimentally infected with Trypanosoma brucei brucei
  24. Acacia arabica (Lam.) Willd. On osteoblastogenesis, osteoblast proliferation, osteoclastic activity, and bone calcium mineralization
  25. GC-MS analysis of curculigo orchiodes and medicinal herbs with cytotoxic, hepatoprotective attributes of ethanolic extract from Indian origin
  26. Biochemical and histopathological evaluation of liver, kidney, and pancreas in normal Sprague Dawley rats administered aqueous extract of beetroot (Beta vulgaris)
  27. The effectiveness of emotional freedom techniques (EFT) on depression of postmenopausal women: a randomized controlled trial
  28. The formulation and efficacy of topical Dorema ammoniacum in treating Melasma: a randomized double-blind, placebo-controlled trial
  29. Impact of inhalation aromatherapy with Damask Rose (Rosa damascena) on stress, anxiety and hemodynamic parameters of patients undergoing coronary angiography: a single blind randomized clinical trial
  30. Hydro-alcoholic extract of Morus nigra reduces fasting blood glucose and HbA1c% in diabetic patients, probably via competitive and allosteric interaction with alpha-glucosidase enzyme; a clinical trial and in silico analysis
  31. Prophylactic management of radiation-induced mucositis using herbal mouthwash in patients with head and neck cancer: an assessor-blinded randomized controlled trial
  32. Implementation science in New Zealand naturopathic practice: a cross-sectional study
  33. Issues of mindfulness implementation for oncology caregivers
  34. Immediate effect of cold mud pack therapy on autonomic variables in primary hypertensive individuals: a randomized trial
  35. Effect of a Persian metabolic diet on the functional dyspepsia symptoms in patients with postprandial distress syndrome: a randomized, double-blind clinical trial
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