GC-MS analysis of curculigo orchiodes and medicinal herbs with cytotoxic, hepatoprotective attributes of ethanolic extract from Indian origin

Vedpal Singh; Namrita Lall; Ashish Wadhwani; Sangai P. Dhanabal

doi:10.1515/jcim-2020-0545

Article

GC-MS analysis of curculigo orchiodes and medicinal herbs with cytotoxic, hepatoprotective attributes of ethanolic extract from Indian origin

Vedpal Singh , Namrita Lall , Ashish Wadhwani and Sangai P. Dhanabal

Published/Copyright: March 23, 2022

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From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 3

Abstract

Objectives

Liver illnesses are a major public health issue all over the world. Medicinal plants constituents a viable alternative for the development of phytopharmaceuticals with hepatoprotective activity in order to solve some of these health-related problems. The present study is focused on the phytochemical and biological investigation on Indian traditional medicinal plant extracts, for their cytotoxic and hepatoprotective activity. The isolated compounds showed the presence of phenolic constituents which lead to cytotoxicity and hepatoprotective activity of medicinal plant. Cancer causes about 13% of all human deaths in 2007 (7.6 million) (American Cancer Society and WHO December 2006–07). The American Cancer Society estimates that 12,990 new cases of cervical cancer will be diagnosed in the United States year 2016. Cancer-related deaths are expected to increase, with an estimated 11.4 million deaths in 2030.

Methods

The ethanolic extracts of Centella asiatica, Myristica fragrans, Trichosanthes palmata, Woodfordia fruticosa, Curculigo orchioides were evaluated against HEP-G2 cell lines for hepatoprotective activity and Curculigo orchioides was further promoted for the isolation of secondary metabolites based on inhibitory concentration.

Results

The ethanolic extracts of C. asiatica, M. fragrans, T. palmata, W. fruticosa, Curculigo orchioides shown significant cytotoxic activity (IC₅₀≤100 μg/mL). The plant extracts also shown significant hepatoprotective activity in a dose dependent manner when tested against HEP-G2 cell lines and cytotoxicity studies against HeLa and HEP-G2 cells.

Conclusions

The extract of Curculigo orchiodes rhizome showed significant cytotoxicity results. Hence the Curculigo orchiodes rhizome was selected for further phytochemical studies to isolate active compounds and their Characterization by GCMS.

Keywords: GCMS; HeLa cell lines; HEP-G2 cell lines; medicinal plants; MTT assay; silymarin

Corresponding author: Dr. Vedpal Singh, Asst. Professor, Department of Pharmacognosy, College of Pharmacy, JSS Academy of Technical Education, C-1/A, Sector 62, Noida, UP, India, Phone: +919927411605, E-mail: vsphd@jssuni.edu.in

Funding source: Department of Science and Technology, Government of India, New Delhi, India

Award Identifier / Grant number: DST/INT/SOUTHAFRICA/P/05/2014-06/2014

Acknowledgment

The authors are grateful to the Department of Science and Technology of Govt. of India, Medical Council Research South Africa, University of Pretoria, and JSS College of Pharmacy, Ooty, JSS Academy of Higher Education & Research, Mysuru, India for their assistance in conducting this study and also thankful to department of Pharmacognosy, College of Pharmacy, JSS Academy of Technical Education, Noida, UP, India.

Research funding: The financial assistance was provided by the Department of Science and Technology, Govt. of India, New Delhi, India, (DST/INT/SOUTHAFRICA/P/05/2014-06/2014).
Author contributions: Vedpal and Ashish Wadhwani participated to the experimental work and text writing, Namrita Lall contributed to the interpretation section, and SP Dhanabal assessed the paper. All authors have read and approved the final manuscript for submission.
Competing interests: The authors reported no conflicts of interest
Ethical approval: The authors declare that experimentation were performed on animals for this study as per the ethical norms and IAEC guidelines.

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Received: 2020-12-31

Accepted: 2022-02-27

Published Online: 2022-03-23

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Articles in the same Issue

https://doi.org/10.1515/jcim-2020-0545

Keywords for this article

GCMS; HeLa cell lines; HEP-G2 cell lines; medicinal plants; MTT assay; silymarin