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Screening of anti-cancerous potential of classical Raudra rasa and modified Raudra rasa modified with hiraka bhasma (nanodiamond) through FTIR & LC-MS analysis

  • Manoj Kumar Dash , Namrata Joshi , Vd. Sushil Dubey , Kamal Nayan Dwivedi and Dev Nath Singh Gautam EMAIL logo
Published/Copyright: January 24, 2022
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 19 Issue 3

Abstract

Objectives

Raudra rasa is an ayurvedic medicine explicitly prescribed for the treatment of arbuda (cancer), whereas hiraka bhasma has the potential to promote cancer healing properties. Together, these two medicines provide multifunction benefits. This paper analyses the functional groups of Raudra rasa modified with hiraka bhasma and compares it with the classically prepared raudra rasa. To identify the functional group, organic ligands, and active compounds present in samples of raudra rasa (CRR) and modified raudra rasa with hiraka bhasma (MRR) contributing to cancer alleviation by using Fourier transform infrared spectroscopy (FTIR) & LC-MS analysis.

Methods

Classical raudra rasa (CRR), its ingredients, shadguna kajjali (SK); decoction of Piper betel Linn. (PBD); Amaranthus spinosus Linn. (ASD); Boerhaavia diffusa Linn. (BDD); Piper longum Linn. (PLD); cow urine (GM), & similarly modified raudra rasa (MRR), its ingredients, hiraka bhasma (HB); shadguna rasasindura (SHR); water-soluble extract of Piper betel Linn. (PBE); Amaranthus spinosus Linn. (ASE); Boerhaavia diffusa Linn. (BDE); cow urine ark (GA); Piper Longum Linn. (PLE) were subjected to FTIR and LC-MS analysis.

Results

Among all 15 samples studied, maximum numbers of peaks (21) were seen in MRR indicating a greater number of functional groups. Further, in MRR, a maximum peak in the double bond region is suggestive of its higher stability compared to CRR. Both the compound is preliminarily a mixture of the number of functional groups like; fluoro, methyl, amino, hydroxy, nitro, methylamino, carbonyl, and iodo groups, having known anti-proliferative activities. By the FT-IR analysis, the biologically active compounds in aqueous and methanol extract of CRR & MRR were identified that have anti-cancerous compounds. In the present study, a total of 40 major compounds like alkaloids, amino acid, carboxylic acid, Flavonoids, Nucleoside, Nucleotide, phenylpropanoid, Sphingosine, stilbenoid, sugar, phosphate, terpenoids, vitamin from aqueous & methanol extract of CRR & MRR were identified by LC-MS.

Conclusions

This research paper highlights the presence of different functional groups and bioactive compounds known to have anti-cancer activities. Thus, this review suggests future recommendations for the design and development of improved anticancer drugs with higher efficacy.

Keywords: anticancer; functional groups; LC-MS; Raudra rasa
Corresponding author: Dr. Dev Nath Singh Gautam, Professor, Department of Rasashastra, Faculty of Ayurveda, IMS, BHU, Varanasi, India, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The local Institutional Review Board.

References

1. Liu, Y. Recent progress in Fourier transform infrared (FTIR) spectroscopy study of compositional, structural and physical attributes of developmental cotton fibers. Materials 2013;6:299–313. https://doi.org/10.3390/ma6010299.Search in Google Scholar

2. Comnea-Stancu, IR, Wieland, K, Ramer, G, Schwaighofer, A, Lendl, B. On the identification of rayon/viscose as a major fraction of microplastics in the marine environment: discrimination between natural and manmade cellulosic fibers using Fourier transform infrared spectroscopy. Appl Spectrosc 2017;71:939–50. https://doi.org/10.1177/0003702816660725.Search in Google Scholar

3. Govindadas, S, Ratnavalli, B. Chapter 44, shloka no 59-60, edited by Kaviraj Shri Ambikadatta Shastri and by Shri Rajeswaradatta Shastri (2002). Varanasi: Chaukambha Sanskrit Samsthana; 2002:583 p.Search in Google Scholar

4. Madamsetty, VS, Pal, K, Keshavan, S, Caulfield, TR, Dutta, SK, Wang, E, et al.. Development of multi-drug loaded PEGylated nanodiamonds to inhibit tumor growth and metastasis in genetically engineered mouse models of pancreatic cancer. Nanoscale 2019;11:22006–18. https://doi.org/10.1039/c9nr05478b.Search in Google Scholar

5. Gupta, C, Prakash, D, Gupta, S. Cancer treatment with nano-diamonds. Front Biosci (Schol Ed) 2017;9:62–70. https://doi.org/10.2741/s473.Search in Google Scholar

6. Kulkarni, DA, editor. (reprint ed.) Hindi commentary of Rasaratna sammuchhaya of Vagbhatta, Paribhasha: Chapter 8, Verse 5. New Delhi: Meharchand Laxamandas Publication; 1998:145 p.Search in Google Scholar

7. Sadananda, SS, Kashinath, S, editors. Rasa Tarangini, 11th ed., Ch. 6, Ver. 189. New Delhi: Motilal Banarasidas; 2004:140 p.Search in Google Scholar

8. Department of Indian Systems of Medicine and Homoeopathy, Ministry of Health and Family Welfare, Government of India. 1st ed. Part I. Department of Indian Systems of Medicine and Homoeopathy, Ministry of Health and Family Welfare, Government of India; New Delhi: Ayurvedic Formulary of India; 2003:636 p.Search in Google Scholar

9. Coates, J. Interpretation of infrared spectra, a practical approach. In: Encyclopedia of analytical chemistry. Chichester: John Wiley & Sons Ltd.; 2000, vol. 12:10815–37 pp.10.1002/9780470027318.a5606Search in Google Scholar

10. Wood, BR, Quinn, MA, Tait, B, Ashdown, M, Hislop, T, Romeo, M, et al.. FTIR microspectroscopic study of cell types and potential confounding variables in screening for cervical malignancies. Biospectroscopy 1998;4:75–91. https://doi.org/10.1002/(sici)1520-6343(1998)4:2<75::aid-bspy1>3.0.co;2-r.10.1002/(SICI)1520-6343(1998)4:2<75::AID-BSPY1>3.0.CO;2-RSearch in Google Scholar

11. Yang, X, Shi, Q, Liu, YN, Zhao, G, Bastow, KF, Lin, JC, et al.. Antitumor agents 268. Design, synthesis, and mechanistic studies of new 9-substituted phenanthrene-based tylophorine analogues as potent cytotoxic agents. J Med Chem 2009;52:5262–8. https://doi.org/10.1021/jm9009263.Search in Google Scholar

12. Petit, T, Puskar, L. FTIR spectroscopy of nanodiamonds: methods and interpretation. Diam Relat Mater 2018;89:52–66. ISSN 0925-9635. https://doi.org/10.1016/j.diamond.2018.08.005.Search in Google Scholar

13. Jin, L, Wang, ML, Lv, Y, Zeng, XY, Chen, C, Ren, H, et al.. Design and synthesis of flavonoidal ethers and their anti-cancer activity in vitro. Molecules 2019;24:1749. https://doi.org/10.3390/molecules24091749.Search in Google Scholar

14. Zhao, D, Luo, H, Chen, B, Chen, W, Zhang, G, Yu, Y. Palladium-catalyzed H/D exchange reaction with 8-aminoquinoline as the directing group: access to ortho-selective deuterated aromatic acids and β-selective deuterated aliphatic acids. J Org Chem 2018;83:7860–6. https://doi.org/10.1021/acs.joc.8b00734.Search in Google Scholar

15. Foudah, AI, Jain, S, Busnena, BA, El Sayed, KA. Optimization of marine triterpene sipholenols as inhibitors of breast cancer migration and invasion. ChemMedChem 2013;8:497–510. https://doi.org/10.1002/cmdc.201200516.Search in Google Scholar PubMed

16. Xia, Y, Chen, R, Lu, G, Li, C, Lian, S, Kang, TW, et al.. Natural phytochemicals in bladder cancer prevention and therapy. Front Oncol 2021;11:652033. https://doi.org/10.3389/fonc.2021.652033.Search in Google Scholar PubMed PubMed Central

17. Jiang, JL, Jin, XL, Zhang, H, Su, X, Qiao, B, Yuan, YJ. Identification of antitumor constituents in curcuminoids from Curcuma longa L. based on the composition-activity relationship. J Pharmaceut Biomed Anal 2012;70:664–70. https://doi.org/10.1016/j.jpba.2012.05.011.Search in Google Scholar PubMed

18. Liew, SK, Malagobadan, S, Arshad, NM, Nagoor, NH. A review of the structure-activity relationship of natural and synthetic antimetastatic compounds. Biomolecules 2020;10:138. https://doi.org/10.3390/biom10010138.Search in Google Scholar PubMed PubMed Central

19. Lim, BS, Donahue, JP, Holm, RH. Synthesis and structures of bis(dithiolene)molybdenum complexes related to the active sites of the DMSO reductase enzyme family. Inorg Chem 2000;39:263–73. https://doi.org/10.1021/ic9908672.Search in Google Scholar PubMed

20. Alho, D, Salvador, J, Cascante, M, Marin, S. Synthesis and antiproliferative activity of novel heterocyclic glycyrrhetinic acid derivatives. Molecules 2019;24:766. https://doi.org/10.3390/molecules24040766.Search in Google Scholar PubMed PubMed Central

21. Poff, AM, Ari, C, Arnold, P, Seyfried, TN, D’Agostino, DP. Ketone supplementation decreases tumor cell viability and prolongs survival of mice with metastatic cancer. Int J Cancer 2014;135:1711–20. https://doi.org/10.1002/ijc.28809.Search in Google Scholar PubMed PubMed Central

22. Feng, S, Wang, H, Liu, J, Aa, J, Zhou, F, Wang, G. Multi-dimensional roles of ketone bodies in cancer biology: opportunities for cancer therapy. Pharmacol Res 2019;150:104500. https://doi.org/10.1016/j.phrs.2019.104500.Search in Google Scholar PubMed

23. Song, J, Peng, P, Chang, J, Liu, MM, Yu, JM, Zhou, L, et al.. Selective non-zinc binding MMP-2 inhibitors: novel benzamide Ilomastat analogs with anti-tumor metastasis. Bioorg Med Chem Lett 2016;26:2174–8. https://doi.org/10.1016/j.bmcl.2016.03.064.Search in Google Scholar PubMed

24. Eroğlu, C, Avcı, E, Vural, H, Kurar, E. Anticancer mechanism of Sinapic acid in PC-3 and LNCaP human prostate cancer cell lines. Gene 2018;671:127–34. https://doi.org/10.1016/j.gene.2018.05.049.Search in Google Scholar PubMed

25. Lokman, NA, Ho, R, Gunasegaran, K, Bonner, WM, Oehler, MK, Ricciardelli, C. Anti-tumour effects of all-trans retinoic acid on serous ovarian cancer. J Exp Clin Cancer Res 2019;38:10. https://doi.org/10.1186/s13046-018-1017-7.Search in Google Scholar PubMed PubMed Central

26. Zhou, Y, Beyene, D, Zhang, R, Kassa, A, Ashayeri, E, Sridhar, R. Cytotoxicity of etidronic acid to human breast cancer cells. Ethn Dis 2008;18 (2 Suppl 2):S2–92.Search in Google Scholar

27. Hamed, M, Kalita, D, Bartolo, ME, Jayanty, SS. Capsaicinoids, polyphenols and antioxidant activities of Capsicum annuum: comparative study of the effect of ripening stage and cooking methods. Antioxidants 2019;8:364. https://doi.org/10.3390/antiox8090364.Search in Google Scholar PubMed PubMed Central

28. Zhang, M, Zhang, MW, Zhang, L, Zhang, L. Methyl jasmonate and its potential in cancer therapy. Plant Signal Behav 2015;10: e1062199. https://doi.org/10.1080/15592324.2015.1062199.Search in Google Scholar PubMed PubMed Central

29. Lee, HH, Sanada, S, An, SM, Ye, BJ, Lee, JH, Seo, YK, et al.. LPS-induced NFκB enhanceosome requires TonEBP/NFAT5 without DNA binding. Sci Rep 2016;6:24921. https://doi.org/10.1038/srep24921.Search in Google Scholar PubMed PubMed Central

30. Aggarwal, V, Kashyap, D, Sak, K, Tuli, HS, Jain, A, Chaudhary, A, et al.. Molecular mechanisms of action of tocotrienols in cancer: recent trends and advancements. Int J Mol Sci 2019;20:656. https://doi.org/10.3390/ijms20030656.Search in Google Scholar PubMed PubMed Central

31. Chirumbolo, S. Alpha-bisabolol, not a matter for cancer therapy. Commentary: “Research on the immunosuppressive activity of ingredients contained in sunscreens”. Front Pharmacol 2015;6:96. https://doi.org/10.3389/fphar.2015.00096.Search in Google Scholar PubMed PubMed Central

32. Walczak, K, Wnorowski, A, Turski, WA, Plech, T. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci 2020;77:1531–50. https://doi.org/10.1007/s00018-019-03332-w.Search in Google Scholar PubMed PubMed Central

33. Jung, YY, Hwang, ST, Sethi, G, Fan, L, Arfuso, F, Ahn, KS. Potential anti-inflammatory and anti-cancer properties of farnesol. Molecules 2018;23:2827. https://doi.org/10.3390/molecules23112827.Search in Google Scholar PubMed PubMed Central

34. Majnooni, MB, Fakhri, S, Shokoohinia, Y, Kiyani, N, Stage, K, Mohammadi, P, et al.. Phytochemicals: potential therapeutic interventions against coronavirus-associated lung injury. Front Pharmacol 2020;11:588467. https://doi.org/10.3389/fphar.2020.588467.Search in Google Scholar PubMed PubMed Central

35. Batra, P, Sharma, AK. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech 2013;3:439–59. https://doi.org/10.1007/s13205-013-0117-5.Search in Google Scholar PubMed PubMed Central

36. Sheid, B, Gaetjens, E, Chung, ST, Lerner, LM. Enzymatic formation of potential anticancer and antiviral inosine analogs. Experientia 1996;52:878–81. https://doi.org/10.1007/bf01938874.Search in Google Scholar PubMed

37. Carrillo, C, Cavia, M, Alonso-Torre, SR. Antitumor effect of oleic acid; mechanisms of action: a review. Nutr Hosp 2012;27:1860–5. https://doi.org/10.3305/nh.2012.27.6.6010.Search in Google Scholar PubMed

38. Kuban-Jankowska, A, Gorska-Ponikowska, M, Wozniak, M. Lipoic acid decreases the viability of breast cancer cells and activity of PTP1B and SHP2. Anticancer Res 2017;37:2893–8. https://doi.org/10.21873/anticanres.11642.Search in Google Scholar PubMed

39. Zhang, QH, Hu, JP, Wang, BL, Li, Y. Effects of capsaicin and dihydrocapsaicin on human and rat liver microsomal CYP450 enzyme activities in vitro and in vivo. J Asian Nat Prod Res 2012;14:382–95. https://doi.org/10.1080/10286020.2012.656605.Search in Google Scholar PubMed

40. Ooi, TC, Chan, KM, Sharif, R. Antioxidant, anti-inflammatory, and genomic stability enhancement effects of zinc l-carnosine: a potential cancer chemopreventive agent? Nutr Cancer 2017;69:201–10. https://doi.org/10.1080/01635581.2017.1265132.Search in Google Scholar PubMed

41. Shahzad, S, Mateen, S, Mubeena Mariyath, PM, Naeem, SS, Akhtar, K, Rizvi, W, et al.. Protective effect of syringaldehyde on biomolecular oxidation, inflammation and histopathological alterations in isoproterenol-induced cardiotoxicity in rats. Biomed Pharmacother 2018;108:625–33. https://doi.org/10.1016/j.biopha.2018.09.055.Search in Google Scholar PubMed

42. Xiao, Z, Morris-Natschke, SL, Lee, KH. Strategies for the optimization of natural leads to anticancer drugs or drug candidates. Med Res Rev 2016;36:32–91. https://doi.org/10.1002/med.21377.Search in Google Scholar PubMed PubMed Central

43. Palozza, P, Maggiano, N, Calviello, G, Lanza, P, Piccioni, E, Ranelletti, FO, et al.. Canthaxanthin induces apoptosis in human cancer cell lines. Carcinogenesis 1998;19:373–6. https://doi.org/10.1093/carcin/19.2.373.Search in Google Scholar PubMed

44. Li, G, Huang, Y, Feng, Q, Chen, Y. Tryptophan as a probe to study the anticancer mechanism of action and specificity of α-helical anticancer peptides. Molecules 2014;19:12224–41. https://doi.org/10.3390/molecules190812224.Search in Google Scholar PubMed PubMed Central

45. Veeriah, S, Kautenburger, T, Habermann, N, Sauer, J, Dietrich, H, Will, F, et al.. Apple flavonoids inhibit the growth of HT29 human colon cancer cells and modulate the expression of genes involved in the biotransformation of xenobiotics. Mol Carcinog 2006;45:164–74. https://doi.org/10.1002/mc.20158.Search in Google Scholar PubMed

46. Stanisic, D, Costa, AF, Fávaro, WJ, Tasic, L, Seabra, AB, Duran, N. Anticancer activities of hesperidin and hesperetin in vivo and their potentiality against bladder cancer. J Nanomed Nanotechnol 2018;9:515. https://doi.org/10.4172/2157-7439.1000515.Search in Google Scholar

47. Pal, HC, Sharma, S, Strickland, LR, Agarwal, J, Athar, M, Elmets, CA, et al.. Delphinidin reduces cell proliferation and induces apoptosis of non-small-cell lung cancer cells by targeting EGFR/VEGFR2 signaling pathways. PLoS One 2013;8:e77270. https://doi.org/10.1371/journal.pone.0077270.Search in Google Scholar PubMed PubMed Central

48. Stompor, M. A review on sources and pharmacological aspects of sakuranetin. Nutrients 2020;12:513. https://doi.org/10.3390/nu12020513.Search in Google Scholar PubMed PubMed Central

49. Kim, YK, Kim, YS, Choi, SU, Ryu, SY. Isolation of flavonol rhamnosides from Loranthus tanakae and cytotoxic effect of them on human tumor cell lines. Arch Pharm Res (Seoul) 2004;27:44–7. https://doi.org/10.1007/bf02980044.Search in Google Scholar PubMed

50. Wang, J, Fang, X, Ge, L, Cao, F, Zhao, L, Wang, Z, et al.. Antitumor, antioxidant and anti-inflammatory activities of kaempferol and its corresponding glycosides and the enzymatic preparation of kaempferol. PLoS One 2018;13: e0197563. https://doi.org/10.1371/journal.pone.0197563.Search in Google Scholar PubMed PubMed Central

51. Tsiklauri, L, An, G, Ruszaj, DM, Alaniya, M, Kemertelidze, E, Morris, ME. Simultaneous determination of the flavonoids robinin and kaempferol in human breast cancer cells by liquid chromatography-tandem mass spectrometry. J Pharmaceut Biomed Anal 2011;55:109–13. https://doi.org/10.1016/j.jpba.2010.12.021.Search in Google Scholar PubMed

52. Liao, MT, Wu, CC, Wu, SV, Lee, MC, Hu, WC, Tsai, KW, et al.. Resveratrol as an adjunctive therapy for excessive oxidative stress in aging COVID-19 patients. Antioxidants 2021;10:1440. https://doi.org/10.3390/antiox10091440.Search in Google Scholar PubMed PubMed Central

53. Mironova, GD, Khrenov, MO, Talanov, EY, Glushkova, OV, Parfenyuk, SB, Novoselova, TV, et al.. The role of mitochondrial KATP channel in anti-inflammatory effects of uridine in endotoxemic mice. Arch Biochem Biophys 2018;654:70–6. https://doi.org/10.1016/j.abb.2018.07.006.Search in Google Scholar PubMed

54. Kosic, M, Paunovic, V, Ristic, B, Mircic, A, Bosnjak, M, Stevanovic, D, et al.. 3-Methyladenine prevents energy stress-induced necrotic death of melanoma cells through autophagy-independent mechanisms. J Pharmacol Sci 2021;147:156–67. https://doi.org/10.1016/j.jphs.2021.06.003.Search in Google Scholar PubMed

55. Shay, J, Elbaz, HA, Lee, I, Zielske, SP, Malek, MH, Hüttemann, M. Molecular mechanisms and therapeutic effects of (-)-epicatechin and other polyphenols in cancer, inflammation, diabetes, and neurodegeneration. Oxid Med Cell Longev 2015:181260. https://doi.org/10.1155/2015/181260.Search in Google Scholar PubMed PubMed Central

56. Tuli, HS, Tuorkey, MJ, Thakral, F, Sak, K, Kumar, M, Sharma, AK, et al.. Molecular mechanisms of action of Genistein in cancer: recent advances. Front Pharmacol 2019;10:1336. https://doi.org/10.3389/fphar.2019.01336.Search in Google Scholar

57. Tay, KC, Tan, LT, Chan, CK, Hong, SL, Chan, KG, Yap, WH, et al.. A review of its anticancer potentials and mechanisms. Front Pharmacol 2019;10:820. https://doi.org/10.3389/fphar.2019.00820.Search in Google Scholar

58. Dutta, S, Ray, S, Nagarajan, K. Glutamic acid as an anticancer agent: an overview. Saudi Pharm J 2013;21:337–43. https://doi.org/10.1016/j.jsps.2012.12.007.Search in Google Scholar

59. Shen, S, Zhang, Y, Zhang, R, Gong, X. Sarsasapogenin induces apoptosis via the reactive oxygen species-mediated mitochondrial pathway and ER stress pathway in HeLa cells. Biochem Biophys Res Commun 2013;441:519–24. https://doi.org/10.1016/j.bbrc.2013.10.101.Search in Google Scholar

60. Sheid, B, Gaetjens, E, Chung, ST, Lerner, LM. Enzymatic formation of potential anticancer and antiviral inosine analogs. Experientia 1996;52:878–81. https://doi.org/10.1007/bf01938874.Search in Google Scholar

61. Matsuo, T, Sadzuka, Y. In vitro anticancer activities of B6 vitamers: a mini-review. Anticancer Res 2019;39:3429–32. https://doi.org/10.21873/anticanres.13488.Search in Google Scholar

62. Kim, CS, Kawada, T, Kim, BS, Han, IS, Choe, SY, Kurata, T, et al.. Capsaicin exhibits anti-inflammatory property by inhibiting IkB-a degradation in LPS-stimulated peritoneal macrophages. Cell Signal 2003;15:299–306. https://doi.org/10.1016/s0898-6568(02)00086-4.Search in Google Scholar

63. Clark, R, Lee, SH. Anticancer properties of capsaicin against human cancer. Anticancer Res 2016;36:837–43.Search in Google Scholar
Received: 2021-09-10
Accepted: 2021-12-29
Published Online: 2022-01-24

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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  2. Reviews
  3. Evidence implementation among complementary medicine practitioners: a meta-summary
  4. Phytochemical: a treatment option for heavy metal induced neurotoxicity
  5. The effects of curcumin-containing supplementations on inflammatory markers and lipid profiles in patients with chronic kidney diseases: a systematic review and meta-analysis of randomized controlled trials
  6. A systematic review on traditional medicinal Animal’s parts and products used for the treatment of respiratory tract disorders in Ethiopia
  7. Review on nutraceuticals: phase transition from preventive to protective care
  8. A study of effect of Centella asiatica on oxidative markers in the hippocampus of offsprings born to alcohol-fed pregnant rats and the correlation with their cognitive functions
  9. Anxiogenic and anxiolytic effects of memantine injected into the ventral hippocampus in male stressed mice
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  13. Highly efficient microencapsulation of phytonutrients by fractioned cellulose using biopolymer complexation technology
  14. Ocimum basilicum alleviates blood glucose, lipid profile and iNOS in diabetes gestational rat model
  15. Blood pressure and blood sugar-lowering effects of purified gambir on diabetic hypertensive Wistar Kyoto rats
  16. Assessment of anti-inflammatory and antioxidant activity of quercetin–rutin blend (SophorOx™) – an invitro cell based assay
  17. Subchronic toxicological evaluation of EnXtra™ (standardised extract of Alpinia galanga rhizome) in rats
  18. Ficus glumosa Del. reduces phenylhydrazine-induced hemolytic anaemia and hepatic damage in Wistar rats
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  20. Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats
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https://doi.org/10.1515/jcim-2021-0410
Keywords for this article
anticancer; functional groups; LC-MS; Raudra rasa

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Evidence implementation among complementary medicine practitioners: a meta-summary
  4. Phytochemical: a treatment option for heavy metal induced neurotoxicity
  5. The effects of curcumin-containing supplementations on inflammatory markers and lipid profiles in patients with chronic kidney diseases: a systematic review and meta-analysis of randomized controlled trials
  6. A systematic review on traditional medicinal Animal’s parts and products used for the treatment of respiratory tract disorders in Ethiopia
  7. Review on nutraceuticals: phase transition from preventive to protective care
  8. A study of effect of Centella asiatica on oxidative markers in the hippocampus of offsprings born to alcohol-fed pregnant rats and the correlation with their cognitive functions
  9. Anxiogenic and anxiolytic effects of memantine injected into the ventral hippocampus in male stressed mice
  10. Research Articles
  11. Comparison of antithyroid effects and hepatic complications of methimazole with catechin and its nanoencapsulation form in adult male rats
  12. Almond oil restores blood parameters, liver function, blood and liver antioxidants and DNA, and liver histology more efficiently than olive oil in favism
  13. Highly efficient microencapsulation of phytonutrients by fractioned cellulose using biopolymer complexation technology
  14. Ocimum basilicum alleviates blood glucose, lipid profile and iNOS in diabetes gestational rat model
  15. Blood pressure and blood sugar-lowering effects of purified gambir on diabetic hypertensive Wistar Kyoto rats
  16. Assessment of anti-inflammatory and antioxidant activity of quercetin–rutin blend (SophorOx™) – an invitro cell based assay
  17. Subchronic toxicological evaluation of EnXtra™ (standardised extract of Alpinia galanga rhizome) in rats
  18. Ficus glumosa Del. reduces phenylhydrazine-induced hemolytic anaemia and hepatic damage in Wistar rats
  19. Screening of anti-cancerous potential of classical Raudra rasa and modified Raudra rasa modified with hiraka bhasma (nanodiamond) through FTIR & LC-MS analysis
  20. Aqueous extract of Pedalium murex D. Royen ex L. leafy stem protects against lead induced testicular toxicity in Wistar rats
  21. Intrahippocampal co-administration of nicotine and O-acetyl-L-carnitine prevents the H-89-induced spatial learning deficits in Morris water maze
  22. Developmental effects of early-life stress on dopamine D2 receptor and proteins involved in noncanonical D2 dopamine receptor signaling pathway in the prefrontal cortex of male rats
  23. Probiotic administration enhanced antitrypanosomal effects of diminazene aceturate in dogs experimentally infected with Trypanosoma brucei brucei
  24. Acacia arabica (Lam.) Willd. On osteoblastogenesis, osteoblast proliferation, osteoclastic activity, and bone calcium mineralization
  25. GC-MS analysis of curculigo orchiodes and medicinal herbs with cytotoxic, hepatoprotective attributes of ethanolic extract from Indian origin
  26. Biochemical and histopathological evaluation of liver, kidney, and pancreas in normal Sprague Dawley rats administered aqueous extract of beetroot (Beta vulgaris)
  27. The effectiveness of emotional freedom techniques (EFT) on depression of postmenopausal women: a randomized controlled trial
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  29. Impact of inhalation aromatherapy with Damask Rose (Rosa damascena) on stress, anxiety and hemodynamic parameters of patients undergoing coronary angiography: a single blind randomized clinical trial
  30. Hydro-alcoholic extract of Morus nigra reduces fasting blood glucose and HbA1c% in diabetic patients, probably via competitive and allosteric interaction with alpha-glucosidase enzyme; a clinical trial and in silico analysis
  31. Prophylactic management of radiation-induced mucositis using herbal mouthwash in patients with head and neck cancer: an assessor-blinded randomized controlled trial
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  34. Immediate effect of cold mud pack therapy on autonomic variables in primary hypertensive individuals: a randomized trial
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