Anti-proliferation effects of Apatinib in combination with Curcumin in breast cancer cells

Mahdi Farhoudi Sefidan Jadid; Gholamreza Jahangirzadehd; Javad Behroozi

doi:10.1515/hmbci-2022-0036

Article

Anti-proliferation effects of Apatinib in combination with Curcumin in breast cancer cells

Mahdi Farhoudi Sefidan Jadid , Gholamreza Jahangirzadehd and Javad Behroozi

Published/Copyright: September 5, 2022

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 44 Issue 1

Abstract

Objectives

Despite remarkable development of new therapeutic strategies to improve survival rates and treatment of patients with cancer, there are still many limitations in management of patients with distant metastasis breast cancer. Therefore, the aim of this study was to investigate a novel method to enhance therapeutic efficacy of Apatinib (as a chemotherapeutic agent) by co‐administration of Curcumin (as a bioactive herbal compound) in breast cancer treatment.

Methods

Effects of Apatinib, Curcumin, and their combinations (Apa‐Cur) was evaluated on viability and proliferation of breast cell line (MCF7) by MTT assay. Moreover, effects of Apatinib, Curcumin, and Apa‐Cur was investigated on apoptosis rate in the cancer cells. Expression levels of apoptosis-related genes (BAX, SMAC, BCL2, and SURVIVIN) in treated cancer cells and untreated controls were evaluated using the Real-Time PCR method.

Results

The obtained results showed that all treatments of Apatinib, Curcumin, and Apa‐Cur significantly decreased viability and proliferation of the breast cancer cells in a concentration‐ and time‐dependent manner. However, anti-proliferation activity of Apa‐Cur combination was significantly higher than Apatinib and Curcumin treatment alone. In addition, Apatinib, Curcumin, and Apa‐Cur increased apoptosis percentage in the treated cancer cells through regulation of apoptosis-related genes expression.

Conclusions

In general, Apa‐Cur combination therapy exerts more profound anti-proliferation effects on breast cancer cell than Apatinib or Curcumin monotherapy. However, further studies are required to identify other possible signaling pathways and mechanisms involved in the anticancer effects of Apatinib, Curcumin, and Apa‐Cur.

Keywords: Apatinib; apoptosis; breast cancer; Curcumin; gene expression

Corresponding author: Mahdi Farhoudi Sefidan Jadid, Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran, Phone: +98 32889353, E-mail: mahdi.farhoudi76@gmail.com

Acknowledgements

We thank the whole staff of Biotechnology Research Center, Islamic Azad University, Tabriz Branch for assistance in the successful strategy of this research.

Research funding: This Research was funded authors.
Author contributions: MFSJ contributed to data collection, data analyses, and drafting the manuscript; GJ contributed to data collection and drafting the manuscript; JB contributed to the statistical analysis; All authors read and approved the final manuscript.
Competing interests: The authors have no conflicts of interest to declare that are relevant to the content of this article.
Informed consent: None applicable.
Ethics approval and consent to participate: None applicable.
Data availability: The datasets generated and/or analyzed during the current study are not publicly available due the nature and type of research, but are available from the corresponding author on reasonable request.

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Received: 2022-04-09

Accepted: 2022-07-20

Published Online: 2022-09-05

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2022-0036

Keywords for this article

Apatinib; apoptosis; breast cancer; Curcumin; gene expression