Does dexamethasone inhibit glucose oxidase: an analysis in kinetics and molecular study

Edris Majd; Dariush Minai-Tehrani; Hamidreza Mollasalehi

doi:10.1515/hmbci-2022-0030

Article

Does dexamethasone inhibit glucose oxidase: an analysis in kinetics and molecular study

Edris Majd , Dariush Minai-Tehrani and Hamidreza Mollasalehi

Published/Copyright: September 1, 2022

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 44 Issue 1

Abstract

Objectives

Glucose oxidase is an enzyme that is widely used in biosensors, especially kits for measuring blood sugar. Many diabetics use this type of kit to determine their blood sugar level. Aspergillus niger is the most important source of glucose oxidase for use in biosensors. Diabetes causes secondary diseases in patients for which medications are prescribed to improve them. Dexamethasone, a corticosteroid, is one of the drugs prescribed to diabetics to cure some secondary diseases. In this study, the effect of this drug on glucose oxidase was investigated from a kinetic and molecular point of view.

Methods

In this study, the kinetics of drug binding to the enzyme was measured and the type of inhibition was determined by Lineweaver-Burk plot. The Ki value of the drug was determined by drawing the secondary curve. Using fluorescence spectrophotometry and molecular docking, the binding of the drug to the enzyme was confirmed.

Results

The results showed that the drug inhibits the enzyme non-competitively. Determining the kinetics parameters of the drug-enzyme interaction showed that the drug acts as a potent inhibitor. Study at the molecular level by fluorescence spectrophotometer showed that the drug attachment alters the enzyme conformation to more compaction. In silico results showed that the drug is placed between two helices that are outside the active site and binds to the enzyme by three hydrogen bonds.

Conclusions

The result of this study is useful because it suggests that in diabetic patients taking dexamethasone, the amount of glucose declared by the kit may not be real due to the inhibition of glucose oxidase.

Keywords: dexamethasone; drug; enzyme; glucose oxidase; inhibition

Corresponding author: Dariush Minai-Tehrani, PhD, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University (GC), Tehran, Iran, Phone: +98 21 29905916, Fax: +98 21 22431664, E-mail: d_mtehrani@sbu.ac.ir

Research funding: No.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: The local Institutional Review Board deemed the study exempt from review.

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Received: 2022-03-19

Accepted: 2022-07-20

Published Online: 2022-09-01

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2022-0030

Keywords for this article

dexamethasone; drug; enzyme; glucose oxidase; inhibition