Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis
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Shabnam Mostofi
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Seyyed Ali Rahmani
Abstract
Objectives
The aim of this study was to investigate the effects of curcumin on the viability, migration, and apoptosis of A549 lung cancer cells. Furthermore, RECK/MMPs axis as a probable regulator of cancer cell migration was assessed.
Methods
In this study, effect of curcumin on viability changes, cell migration, and percentage of apoptosis of A549 non-small cell lung carcinoma was examined. The methylation status of RECK gene was investigated using MS-HRM technique. Moreover, expression changes of genes involved in apoptosis and migration (including CASP3, CASP8, CASP9, BAX, BCL2, MMP9, MMP2, and RECK) were investigated by quantitative Real-Time PCR.
Results
The results of MTT assay showed that the cytotoxic effect of curcumin was in a dose dependent manner. Flow cytometry results demonstrated a significant increase in the percentage of apoptotic cells in curcumin treated group. In addition, curcumin inhibited migration rate in lung cancer cells. qRT-PCR revealed that expression of the candidate genes was in line with suppressed growth and migration. This could be due to, decreased methylation of the RECK gene promoter after curcumin treatment.
Conclusions
Curcumin inhibited lung cancer cells through various molecular pathways. RECK/MMPs axis as a regulator of cancer cell migration was modulated after curcumin treatment and invasion of lung cancer cells was decreased.
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Research funding: Not applicable.
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Author contributions: Conceived and designed the experiments: SM and DS; Performed the experiments: SM and MA; Statistical analysis: SAR; Wrote the paper: SM and DS. All authors read and approved the final manuscript.
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Competing interests: The author declares that there is no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: Not applicable.
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Data availability: The data sets used and analyzed during the current study are available from the corresponding author on reasonable request.
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- COVID-19 pandemic: transmission of SARS-CoV-2 in animals, a risk for human beings
- Original Articles
- Anti Mullerian hormone as a diagnostic tool for polycystic ovary syndrome in women of reproductive age with morbid obesity
- Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6)
- The investigation of the frequency of the alpha-1-antitrypsin phenotype in patients with liver cirrhosis
- Anti-oxidant effect of metformin through AMPK/SIRT1/PGC-1α/SIRT3– independent GPx1 expression in the heart of mice with endometriosis
- Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients
- Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population
- Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats
- Adrenocorticotropic hormone (ACTH) level and adrenal deficiency in patients with mucocutaneous pemphigus
- The effect of resistance training on serum levels of sex hormones and sperm quality in male rats under X-ray radiation
- The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet
- Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis
- Efficacy of rectal progesterone on maternal and neonatal outcomes in pregnant women with Preterm Premature Rupture of membranes: a triple-blind randomised clinical trial
- Case Report
- Ovarian tissue cryopreservation in Malaysia: a case series
- Review Articles
- An overview of prognostic value of neurologic and cardiac biomarkers in patients with COVID-19 sequelae
- Potential role of melatonin in prevention and treatment of lung cancer
- Esketamine–A quick-acting novel antidepressant without the disadvantages of ketamine
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- COVID-19 pandemic: transmission of SARS-CoV-2 in animals, a risk for human beings
- Original Articles
- Anti Mullerian hormone as a diagnostic tool for polycystic ovary syndrome in women of reproductive age with morbid obesity
- Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6)
- The investigation of the frequency of the alpha-1-antitrypsin phenotype in patients with liver cirrhosis
- Anti-oxidant effect of metformin through AMPK/SIRT1/PGC-1α/SIRT3– independent GPx1 expression in the heart of mice with endometriosis
- Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients
- Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population
- Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats
- Adrenocorticotropic hormone (ACTH) level and adrenal deficiency in patients with mucocutaneous pemphigus
- The effect of resistance training on serum levels of sex hormones and sperm quality in male rats under X-ray radiation
- The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet
- Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis
- Efficacy of rectal progesterone on maternal and neonatal outcomes in pregnant women with Preterm Premature Rupture of membranes: a triple-blind randomised clinical trial
- Case Report
- Ovarian tissue cryopreservation in Malaysia: a case series
- Review Articles
- An overview of prognostic value of neurologic and cardiac biomarkers in patients with COVID-19 sequelae
- Potential role of melatonin in prevention and treatment of lung cancer
- Esketamine–A quick-acting novel antidepressant without the disadvantages of ketamine
