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Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population

  • Dimitra Dedousi , Despoina Mavrogianni , Myrto Papamentzelopoulou ORCID logo EMAIL logo , Sofoklis Stavros ORCID logo , Rami Raouasnte , Dimitris Loutradis and Peter Drakakis
Published/Copyright: July 4, 2022
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Hormone Molecular Biology and Clinical Investigation
From the journal Hormone Molecular Biology and Clinical Investigation Volume 43 Issue 4

Abstract

Objectives

The present case-control study investigates whether TP53 Arg72Pro variant (rs1042522) serves as a risk factor for recurrent pregnancy loss (RPL) in Greek women.

Methods

The study group consisted of 100 patients with at least two miscarriages of unexplained etiology, before the 24th week of gestation. The control group included 106 women with no pregnancy loss history. DNA was extracted and genotyped using specific primers for PCR amplification of the Arg72 and Pro72 alleles. Sanger sequencing was used for the discrimination between heterozygotes and homozygotes for Arg72Pro variant.

Results

This is the first study demonstrating the statistically significant higher frequency of TP53 Arg72Pro variant in Greek RPL women compared to controls (38% vs. 6.6%; OR=8.6682, 95% CI: 3.6446–20.6160; p<0.0001). GC genotype (Arg/Pro) and CC genotype (Pro/Pro) were statistically more common in RPL patients than in controls (16% vs. 1.9%; p=0.0027, and 22 vs. 4.7%; p=0.0008, respectively). C allele frequency was statistically significant higher in RPL group than in controls (30.0 vs. 5.7%; p<0.0001). According to the inheritance mode analysis, the model that best fit the data was the dominant model (OR=8.67, 95% CI=3.64–20.62; p<0.0001).

Conclusions

The is the first study disclosing strong evidence that TP53 rs1042522 is significantly associated with a higher risk for recurrent pregnancy loss in Greek women following a dominant model, thus, serving as a genetic marker for identifying women at increased risk of recurrent miscarriages.

Keywords: allele frequencies; Arg72Pro variant; genotyping; inheritance models; recurrent pregnancy loss
Corresponding author: Dr. Myrto Papamentzelopoulou, Molecular Biology Unit, Division of Human Reproduction, 1st Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Athens, 80, Vasilissis Sofias Ave., 11528, Greece, Phone: +30 2132162627, Fax: +30 2132162898, E-mail:

Acknowledgments

We would like to acknowledge the contribution of all recruited patients in the present study.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The local Institutional Review Board deemed the study exempt from review.

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Received: 2021-11-13
Accepted: 2022-06-11
Published Online: 2022-07-04

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

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  2. Letter to the Editor
  3. COVID-19 pandemic: transmission of SARS-CoV-2 in animals, a risk for human beings
  4. Original Articles
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  9. Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients
  10. Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population
  11. Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats
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  13. The effect of resistance training on serum levels of sex hormones and sperm quality in male rats under X-ray radiation
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  15. Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis
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  17. Case Report
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  19. Review Articles
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https://doi.org/10.1515/hmbci-2021-0093
Keywords for this article
allele frequencies; Arg72Pro variant; genotyping; inheritance models; recurrent pregnancy loss

Articles in the same Issue

  1. Frontmatter
  2. Letter to the Editor
  3. COVID-19 pandemic: transmission of SARS-CoV-2 in animals, a risk for human beings
  4. Original Articles
  5. Anti Mullerian hormone as a diagnostic tool for polycystic ovary syndrome in women of reproductive age with morbid obesity
  6. Potential tumor marker for hepatocellular carcinoma identification: PI3K and pro-inflammatory cytokines (TGF-β, IL-1, and IL-6)
  7. The investigation of the frequency of the alpha-1-antitrypsin phenotype in patients with liver cirrhosis
  8. Anti-oxidant effect of metformin through AMPK/SIRT1/PGC-1α/SIRT3– independent GPx1 expression in the heart of mice with endometriosis
  9. Hypoxia-inducible factor-1α and ischemia-modified albumin levels in intensive care COVID-19 Patients
  10. Association between TP53 Arg72Pro variant and recurrent pregnancy loss in the Greek population
  11. Selective estrogen receptor α and β antagonist aggravate cardiovascular dysfunction in type 2 diabetic ovariectomized female rats
  12. Adrenocorticotropic hormone (ACTH) level and adrenal deficiency in patients with mucocutaneous pemphigus
  13. The effect of resistance training on serum levels of sex hormones and sperm quality in male rats under X-ray radiation
  14. The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet
  15. Anti-migratory effect of curcumin on A-549 lung cancer cells via epigenetic reprogramming of RECK/ matrix metalloproteinase axis
  16. Efficacy of rectal progesterone on maternal and neonatal outcomes in pregnant women with Preterm Premature Rupture of membranes: a triple-blind randomised clinical trial
  17. Case Report
  18. Ovarian tissue cryopreservation in Malaysia: a case series
  19. Review Articles
  20. An overview of prognostic value of neurologic and cardiac biomarkers in patients with COVID-19 sequelae
  21. Potential role of melatonin in prevention and treatment of lung cancer
  22. Esketamine–A quick-acting novel antidepressant without the disadvantages of ketamine
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