The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet

Ali Delpisheh; Alireza Safarzade

doi:10.1515/hmbci-2021-0107

Article

The effect of high-intensity interval training on serum and adipose tissues vaspin levels in rats fed a high-fat high-sucrose diet

Ali Delpisheh and Alireza Safarzade

Published/Copyright: July 7, 2022

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 43 Issue 4

Abstract

Background

Vaspin is an adipocytokine with insulin-sensitizing and anti-inflammatory traits.

Objectives

The purpose of this study was to evaluate the effect of high-intensity interval training (HIIT) on serum, visceral and subcutaneous adipose tissue vaspin levels in rats exposed to a diet high in fat and sugar (HFS).

Materials and methods

Thirty-two male Wistar rats were randomly divided into HFS and standard diet (SD) groups. After 12 weeks, each group was divided into sedentary and HIIT groups. HIIT program was performed 3 times/week for 8 weeks. Retroperitoneal adipose tissue, inguinal adipose tissue and serum were collected to analyze vaspin levels. Also, serum glucose and insulin levels, insulin resistance index (HOMA-IR) and retroperitoneal and inguinal fat weights were measured.

Results

HFS significantly increased weight gain, weight of inguinal (p=0.001) and retroperitoneal fat depots (p<0.001), serum glucose levels (p<0.001) and HOMA-IR (p<0.001). The HIIT was able to decline weight gain and fat mass (p<0.05) but did not affect inguinal and retroperitoneal fat depots’ vaspin levels. Eight weeks’ HIIT significantly increased serum vaspin (p=0.002) and decreased insulin (p=0.001) levels only in rats fed with SD.

Conclusions

Although the HIIT program can cause significantly reducing effects on weight gain and fat depots’ weights, it does not effect on circulating and fat depots’ vaspin levels in rats fed an HFS.

Keywords: adipokines; high fat diet; HIIT; insulin resistance; white fat depots

Corresponding author: Alireza Safarzade, Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran; and Athletic Performance and Health Research Center, University of Mazandaran, Babolsar, Iran, E-mail: a.safarzade@umz.ac.ir

Funding source: University of Mazandaran

Award Identifier / Grant number: Unassigned

Acknowledgments

The authors would like to thank the staff of the Animal Laboratory of the University of Mazandaran for their sincere support.

Research funding: None declared.
Author contributions: All authors have participated in the conception and design of this work or the analysis and interpretation of the data.
Competing interests: The authors have no conflicts of interest to declare.
Ethical approval: All procedures were approved by the University of Mazandaran Ethics Committee, with the code IR.UMZ.REC.1397.081.
Availability of data and material: Not applicable.
Code availability: Not applicable.
Consent to participate: Not applicable.

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Received: 2021-12-20

Accepted: 2022-06-11

Published Online: 2022-07-07

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2021-0107

Keywords for this article

adipokines; high fat diet; HIIT; insulin resistance; white fat depots