Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in Alzheimer’s disease
-
Agatha Grela
Agatha Grela obtained her MSc at the Jagiellonian University, Krakow, in analytical chemistry (2010) and she is a PhD student there. Her master’s degree research was undertaken at the Laboratory of Analytical Toxicology and Monitored Therapy (Department of Toxicology and Environmental Diseases) at the Medical College of the Jagiellonian University. In 2010, she had a monthly internship in the Crime Lab of Criminal Intelligence Service Austria. Her prime interest is metabolomics in neuropsychiatric diseases, especially depression.
Agata Turek obtained her BSc at the Jagiellonian University, Krakow, in analytical chemistry (2011), where she is a master’s student. In 2011, she won a scholarship that enabled her to do a monthly internship at the Department of Forensic Medicine of the Medical University of Silesia in Katowice. Additionally to studying chemistry, she studies medical analytics at the Faculty of Pharmacy of the Jagiellonian University.
Wojciech Piekoszewski is a Professor of Medical Sciences with a second degree specialization in Toxicology. He is the Head of the Toxicological and Pharmaceutical Analysis Group in the Department of Analytical Chemistry and the Head of the Laboratory of High-Resolution Mass Spectrometry, Regional Laboratory of Physicochemical Analysis and Structural Research of the Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
He graduated and obtained his PhD and DSc at the Medical College of the Jagiellonian University. He spent 6 months at the Institute of Toxicology and Pharmacology at the University of Wurzburg, Germany, on postdoctoral fellowship, and 30 months as a visiting Professor at the Department of Pharmaceutical Sciences at the State University of New York at Buffalo.
W. Piekoszewski’s scientific interests are in the application of metabolomics and lipidomics as tools for diagnosis of mental and neurodegenerative disorders, and analysis of drugs and medicaments in biological materials. He is a European Registered Toxicologist.
To date he has authored over 100 scientific publications.
Abstract
Alzheimer’s disease is becoming an increasing problem in our aging society. According to our knowledge, so far, no effective pharmacotherapy to cure the cause of the disease has been developed. Therefore, early diagnosis is needed, which will result in implementation of a drug therapy aimed at decreasing and/or inhibiting disease development. Mass spectrometry techniques (MS) have a wide range of applications in proteomics and the search for biomarkers of neurodegenerative disorders, opening new possibilities in diagnostics. Identification of proteins in body fluids (like cerebrospinal fluid or blood) is possible due to MS spectra analysis. The detected changes in protein concentrations are connected with pathological states in an organism and, therefore, can be regarded as biomarkers. Developing procedures for proteome analysis might result in fast diagnosis, as well as creating better suited pharmaceuticals. This paper reviews the search of biomarkers in cerebrospinal fluid and blood. Later on, the use of matrix-assisted-laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in proteomics, focusing on blood-related biomarkers, is discussed. The aim of the work is also to highlight the advantages and disadvantages of MALDI-TOF-based analyses.
About the authors
Agatha Grela obtained her MSc at the Jagiellonian University, Krakow, in analytical chemistry (2010) and she is a PhD student there. Her master’s degree research was undertaken at the Laboratory of Analytical Toxicology and Monitored Therapy (Department of Toxicology and Environmental Diseases) at the Medical College of the Jagiellonian University. In 2010, she had a monthly internship in the Crime Lab of Criminal Intelligence Service Austria. Her prime interest is metabolomics in neuropsychiatric diseases, especially depression.
Agata Turek obtained her BSc at the Jagiellonian University, Krakow, in analytical chemistry (2011), where she is a master’s student. In 2011, she won a scholarship that enabled her to do a monthly internship at the Department of Forensic Medicine of the Medical University of Silesia in Katowice. Additionally to studying chemistry, she studies medical analytics at the Faculty of Pharmacy of the Jagiellonian University.
Wojciech Piekoszewski is a Professor of Medical Sciences with a second degree specialization in Toxicology. He is the Head of the Toxicological and Pharmaceutical Analysis Group in the Department of Analytical Chemistry and the Head of the Laboratory of High-Resolution Mass Spectrometry, Regional Laboratory of Physicochemical Analysis and Structural Research of the Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
He graduated and obtained his PhD and DSc at the Medical College of the Jagiellonian University. He spent 6 months at the Institute of Toxicology and Pharmacology at the University of Wurzburg, Germany, on postdoctoral fellowship, and 30 months as a visiting Professor at the Department of Pharmaceutical Sciences at the State University of New York at Buffalo.
W. Piekoszewski’s scientific interests are in the application of metabolomics and lipidomics as tools for diagnosis of mental and neurodegenerative disorders, and analysis of drugs and medicaments in biological materials. He is a European Registered Toxicologist.
To date he has authored over 100 scientific publications.
©2012 by Walter de Gruyter Berlin Boston
Artikel in diesem Heft
- Masthead
- Masthead
- Editorial
- EDTA-dependent pseudothrombocytopenia: further insights and recommendations for prevention of a clinically threatening artifact
- Reviews
- Mitochondria as sources and targets of damage in cellular aging
- Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in Alzheimer’s disease
- Opinion Papers
- The intellectual contribution of laboratory medicine professionals to research papers on laboratory medicine topics published in high-impact general medicine journals
- Laboratory medicine and sports: between Scylla and Charybdis
- Guidelines and Recommendations
- The EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine: version 4 – 2012
- Genetics And Molecular Diagnostics
- Assessing the utility of whole genome amplified DNA as a template for DMET Plus array
- General Clinical Chemistry And Laboratory Medicine
- Effect of acceleration forces during transport through a pneumatic tube system on ROTEM® analysis
- The transferrin/log(ferritin) ratio: a new tool for the diagnosis of iron deficiency anemia
- Instructions on laboratory monitoring in 200 drug labels
- Lipid peroxidation markers in Crohn’s disease: the associations and diagnostic value
- Impact of asthmatic control status on serum cystatin C concentrations
- Elevated levels of Nɛ-homocysteinyl-lysine isopeptide in patients on long-term hemodialysis
- Interleukin-1α gene variants influence bone mineral density and the risk of osteoporotic hip fractures in elderly Slovenian people
- Novel method to dissociate platelet clumps in EDTA-dependent pseudothrombocytopenia based on the pathophysiological mechanism
- Proficiency testing/external quality assurance for the PFA-100®
- Interference of selected flavonoid aglycons in platelet aggregation assays
- Infectious Diseases
- Monocyte chemoattractant protein-1 and paraoxonase-1 and 3 levels in patients with sepsis treated in an intensive care unit: a preliminary report
- Association between ischemia-modified albumin, homocysteine, vitamin B12 and folic acid in patients with severe sepsis
- Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects
- The predictive value of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis
- Hypophosphatemia, fever and prolonged length of hospital stay in seronegative PCR positive patients as compared to seropositive patients with early acute Q fever pneumonia
- Cancer Diagnostics
- Diagnostic value of HE4 for ovarian cancer: a meta-analysis
- Cardiovascular Diseases
- Reactive oxygen metabolites (ROMs) are associated with cardiovascular disease in chronic hemodialysis patients
- Methylenetetrahydrofolate reductase C677T and reduced folate carrier 80 G>A polymorphisms are associated with an increased risk of conotruncal heart defects
- Oxidative stress as a predictor of cardiovascular events in coronary artery disease patients
- Behavioral and clinical correlates of high-sensitivity C-reactive protein in Japanese men and women
- Letters to the Editor
- Hemoglobin Rambam has a constant retention time on the Tosoh G8 and interferes with the measurement of HbA1c
- Measurement of glycated hemoglobin in a patient with homozygous hemoglobin E
- Congress Abstracts
- 56th National Congress of the Hungarian Society of Laboratory Medicine
Artikel in diesem Heft
- Masthead
- Masthead
- Editorial
- EDTA-dependent pseudothrombocytopenia: further insights and recommendations for prevention of a clinically threatening artifact
- Reviews
- Mitochondria as sources and targets of damage in cellular aging
- Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in Alzheimer’s disease
- Opinion Papers
- The intellectual contribution of laboratory medicine professionals to research papers on laboratory medicine topics published in high-impact general medicine journals
- Laboratory medicine and sports: between Scylla and Charybdis
- Guidelines and Recommendations
- The EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine: version 4 – 2012
- Genetics And Molecular Diagnostics
- Assessing the utility of whole genome amplified DNA as a template for DMET Plus array
- General Clinical Chemistry And Laboratory Medicine
- Effect of acceleration forces during transport through a pneumatic tube system on ROTEM® analysis
- The transferrin/log(ferritin) ratio: a new tool for the diagnosis of iron deficiency anemia
- Instructions on laboratory monitoring in 200 drug labels
- Lipid peroxidation markers in Crohn’s disease: the associations and diagnostic value
- Impact of asthmatic control status on serum cystatin C concentrations
- Elevated levels of Nɛ-homocysteinyl-lysine isopeptide in patients on long-term hemodialysis
- Interleukin-1α gene variants influence bone mineral density and the risk of osteoporotic hip fractures in elderly Slovenian people
- Novel method to dissociate platelet clumps in EDTA-dependent pseudothrombocytopenia based on the pathophysiological mechanism
- Proficiency testing/external quality assurance for the PFA-100®
- Interference of selected flavonoid aglycons in platelet aggregation assays
- Infectious Diseases
- Monocyte chemoattractant protein-1 and paraoxonase-1 and 3 levels in patients with sepsis treated in an intensive care unit: a preliminary report
- Association between ischemia-modified albumin, homocysteine, vitamin B12 and folic acid in patients with severe sepsis
- Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects
- The predictive value of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis
- Hypophosphatemia, fever and prolonged length of hospital stay in seronegative PCR positive patients as compared to seropositive patients with early acute Q fever pneumonia
- Cancer Diagnostics
- Diagnostic value of HE4 for ovarian cancer: a meta-analysis
- Cardiovascular Diseases
- Reactive oxygen metabolites (ROMs) are associated with cardiovascular disease in chronic hemodialysis patients
- Methylenetetrahydrofolate reductase C677T and reduced folate carrier 80 G>A polymorphisms are associated with an increased risk of conotruncal heart defects
- Oxidative stress as a predictor of cardiovascular events in coronary artery disease patients
- Behavioral and clinical correlates of high-sensitivity C-reactive protein in Japanese men and women
- Letters to the Editor
- Hemoglobin Rambam has a constant retention time on the Tosoh G8 and interferes with the measurement of HbA1c
- Measurement of glycated hemoglobin in a patient with homozygous hemoglobin E
- Congress Abstracts
- 56th National Congress of the Hungarian Society of Laboratory Medicine
