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Interference of selected flavonoid aglycons in platelet aggregation assays

  • Mirza Bojić , Željko Debeljak , Marica- Medić-Šarić EMAIL logo and Maja Tomičić
Published/Copyright: February 28, 2012
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 8

Abstract

Background: Flavonoids are widely distributed across the plant kingdom and are therefore common ingredients in an everyday diet. Some flavonoids have a potential to affect platelet aggregation; most often antiaggregatory effects of flavonoids are observed. The objective of this research was to evaluate the in vitro effect of a selected set of flavonoids on platelet aggregation in whole blood.

Methods: The effect of five selected flavonoids (pinocembrin-7-methylether, epicatechin, hesperetin, 6-hydroxyflavone and 3,6-dihydroxyflavone) on platelet aggregation was studied in the citrated whole blood samples collected from 75 healthy volunteers. A Multiplate® impedance analyzer and five different aggregation inducers (ADP, arachidonic acid, collagen, ristocetin and TRAP-6) were utilized for the analysis of samples.

Results: Minimal antiaggregatory concentrations (MINaAC) of flavonoids in individual tests were reported in the following ranges: 0.12–1.91 μM; 15.26–244.14 μM; 15.26–122.07 μM; and 0.06–15.26 μM for ADP, collagen, TRAP-6 and ristocetin aggregation-inducers, respectively. When arachidonic acid was used for induction of platelet aggregation, a proaggregatory effect was observed for pinocembrin-7-methylether, epicatechin, hesperetin and 3,6-dihydroxyflavone, while the expected antiaggregatory effect was observed only for 6-hydroxyflavone (MINaAC=7.63 μM).

Conclusions: Flavonoids interfere with in vitro platelet aggregation assays exhibiting either anti- or proaggregatory effects in concentration ranges that can be achieved in circulation by dietary intake. Thus, dietary intake of flavonoids should be taken into account when interpreting the results of whole blood platelet aggregation.

Keywords: aggregation assays; flavonoids; platelets
Corresponding author: Professor Marica-Medić-Šarić, PhD, Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, University of Zagreb, A. Kovačić 1, 10000 Zagreb, Croatia Phone: +385 1 4818 304, Fax: +385 1 6394 477
Received: 2011-12-29
Accepted: 2012-1-27
Published Online: 2012-02-28
Published in Print: 2012-08-01

©2012 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2011-0960
Keywords for this article
aggregation assays; flavonoids; platelets

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Editorial
  4. EDTA-dependent pseudothrombocytopenia: further insights and recommendations for prevention of a clinically threatening artifact
  5. Reviews
  6. Mitochondria as sources and targets of damage in cellular aging
  7. Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in Alzheimer’s disease
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  10. Laboratory medicine and sports: between Scylla and Charybdis
  11. Guidelines and Recommendations
  12. The EC4 European Syllabus for Post-Graduate Training in Clinical Chemistry and Laboratory Medicine: version 4 – 2012
  13. Genetics And Molecular Diagnostics
  14. Assessing the utility of whole genome amplified DNA as a template for DMET Plus array
  15. General Clinical Chemistry And Laboratory Medicine
  16. Effect of acceleration forces during transport through a pneumatic tube system on ROTEM® analysis
  17. The transferrin/log(ferritin) ratio: a new tool for the diagnosis of iron deficiency anemia
  18. Instructions on laboratory monitoring in 200 drug labels
  19. Lipid peroxidation markers in Crohn’s disease: the associations and diagnostic value
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  21. Elevated levels of Nɛ-homocysteinyl-lysine isopeptide in patients on long-term hemodialysis
  22. Interleukin-1α gene variants influence bone mineral density and the risk of osteoporotic hip fractures in elderly Slovenian people
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  25. Interference of selected flavonoid aglycons in platelet aggregation assays
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  27. Monocyte chemoattractant protein-1 and paraoxonase-1 and 3 levels in patients with sepsis treated in an intensive care unit: a preliminary report
  28. Association between ischemia-modified albumin, homocysteine, vitamin B12 and folic acid in patients with severe sepsis
  29. Evidence for serum miR-15a and miR-16 levels as biomarkers that distinguish sepsis from systemic inflammatory response syndrome in human subjects
  30. The predictive value of immature granulocyte count and immature myeloid information in the diagnosis of neonatal sepsis
  31. Hypophosphatemia, fever and prolonged length of hospital stay in seronegative PCR positive patients as compared to seropositive patients with early acute Q fever pneumonia
  32. Cancer Diagnostics
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