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Identification of a HSP40 gene involved in planarian regeneration

  • Kexue Ma , Hecai Zhang , Yumei Zhang , Gege Song , Meng Wu und Guangwen Chen EMAIL logo
Veröffentlicht/Copyright: 29. Dezember 2017
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Biologia
Aus der Zeitschrift Biologia Band 72 Heft 11

Abstract

Heat shock protein 40 (HSP40), as an essential co-chaperone of HSP70, has been poorly investigated in planarians. In this study, a full-length cDNA of a HSP40 gene from planarian Dugesia japonica (designated Djhsp40) was identified and its expression pattern during planarian regeneration was examined. Its cDNA is 1,378 bp, which includes an open reading frame of 1,236 bp encoding 411 amino acids with a molecular weight of 46.2 kDa. DjHSP40 contains three conserved DNAJ domains belonging to the Type I HSP40/DNAJ family. After amputation is performed, Djhsp40 is up-regulated and the transcripts are specifically expressed in the blastema, and its inhibition by siRNA strongly delays the blastema growth. Interestingly, the silencing of Djhsp40 does not affect the expression level of neoblast-specific proliferation marker (Dj-mcm2 and DjpiwiB), but reduces Dj-mt-mmpA expression significantly. Our work provides a crucial clue for the study of the function of HSP40 in planarian regeneration.

Key words: planarian; HSP40; cDNA cloning; siRNA; regeneration

Acknowledgements

We are grateful to Prof. Wei Wu and Dr. Hanxia Su (School of Life Sciences, Tsinghua University) for technical assistance with the in situ hybridization and microinjection. This work was supported by the National Natural Science Foundation of China (No. 31572267, 31471965, 31702010, 31170357), the Ph.D. Programs Foundation of Ministry of Education of China (No. 200804760003), the Outstanding Young Scientists Foundation of Henan Province (No. 0312001100), the Innovation Foundation of Henan Province (No. 2005126), and the Doctoral Scientific Research Startup Foundation of Henan Normal University (qd14173).

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Abbreviations
EST

expressed sequence tag

HSP

heat shock protein

MMP

matrix metalloproteinases

qPCR

quantitative real-time RT-PCR

RACE

rapid amplification of cDNA ends

siRNA

small interfering RNA

WISH

whole-mount in situ hybridization.

Received: 2017-9-11
Accepted: 2017-11-23
Published Online: 2017-12-29
Published in Print: 2017-11-27

© 2017 Institute of Molecular Biology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2017-0157
Schlagwörter für diesen Artikel
planarian; HSP40; cDNA cloning; siRNA; regeneration

Artikel in diesem Heft

  1. Zoology
  2. Identifying white spots on the roadmap of Late Pleistocene and Holocene palaeolimnology in Slovakia: Review and future directions
  3. Cellular and Molecular Biology
  4. Purification of small-size acidic proteoglycan-like domain of carbonic anhydrase IX fused with thioredoxine expressed in Escherichia coli for structural characterization
  5. Botany
  6. Functional and morphological traits of epiphytic lichens in the Western Carpathian oak forests reflect the influence of air quality and forest history
  7. Botany
  8. Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia
  9. Botany
  10. First signs of old-growth structure and composition of an oak forest after four decades of abandonment
  11. Botany
  12. Factors responsible for the distribution of invasive plant species in the surroundings of railway areas. A case study from SE Poland
  13. Botany
  14. The role of lipids and polysaccharides in model root mucilage with implications for the surface activity of the rhizosphere
  15. Botany
  16. Physiological and proteomic changes in Zizania latifolia under chilling stress
  17. Botany
  18. Novel polymorphic EST-based microsatellite markers characterized in lettuce (Lactuca sativa)
  19. Cellular and Molecular Biology
  20. Identification of a HSP40 gene involved in planarian regeneration
  21. Zoology
  22. A new species of Bothropolys and a new record of Lithobius magnitergiferous (Lithobiidae) from the Qinghai-Tibet Plateau, China
  23. Zoology
  24. Myriapod (Chilopoda, Diplopoda) communities in hedgerows of upland agricultural landscape
  25. Zoology
  26. A new cockroach, with bipectinate antennae,(Blattaria: Olidae fam. nov.) further highlights the differences between the Burmite and other faunas
  27. Zoology
  28. Pre-winter larval activity and feeding behavior of Erebia aethiops and E. cassioides in Austrian Alps
  29. Zoology
  30. Genetic structure of Apis mellifera carnica in Slovakia based on microsatellite DNA polymorphism
  31. Zoology
  32. Amphibians in Czech zoological gardens — trends and implications for conservation
  33. Zoology
  34. Phenetic similarity of European golden jackal (Canis aureus moreoticus) populations from southeastern Europe based on craniometric data
  35. Celluar and Molecular Biology
  36. Fucoidan from Undaria pinnatifida regulates type II collagen and COX-2 expression via MAPK and PI3K pathways in rabbit articular chondrocytes
  37. Zoology
  38. Changes in expression of neurotrophins and neurotrophic factors in the model of eosinophilic inflammation of the esophageal mucosa
  39. Celluar and Molecular Biology
  40. The growth inhibitory effects of garlic polysaccharide combined with cis-dichlorodiamine platinum on human HepG2 cells
  41. Erratum
  42. Reptile surveys reveal high species richness in areas recovering from mining activity in the Brazilian Cerrado
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