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Physiological and proteomic changes in Zizania latifolia under chilling stress

  • Zihong Ye EMAIL logo , Junping Liu , Ye Jin , Haifeng Cui , Xinxin An , Xianshu Fu and Xiaoping Yu
Published/Copyright: December 29, 2017
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Abstract

Zizania latifolia is a perennial aquatic vegetable of the Poaceae family. Chilling temperatures significantly affect stem enlargement and harvest time of Z. latifolia. Cold tolerance improves growth and harvest time at low temperatures. The study aimed to elucidate the proteomics and physiological differences between cold stress-tolerant (Longjiao 2) and -sensitive (911) Z. latifolia varieties. Superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) enzyme activities, as well as levels of malondialdehyde (MDA) and soluble sugars were determined. 2D gel electrophoresis and MOLDI-TOF MS were used to detect the differentially expressed proteins at normal and lower temperatures. Significant differences were observed for relative SOD, CAT, and POD enzyme activities, as well as for soluble sugar levels when comparing the two varieties grown at different temperatures. The results indicated that different responses of the two variety to chilling stress involved previously described physiological mechanisms. Proteomic changes under chilling stress suggested that key pathways including photosynthesis, energy metabolism, defense mechanisms, and RNA processing were differentially regulated in the chilling tolerant variety. Our findings provide a basis for the generation of new varieties Z. latifolia that could be capable of optimal growth under chilling stress, even in the context of global climate change.

Acknowledgements

This work was supported in part by the National Key Technology R&D Program (2016YFF0202300), the National Natural Science Foundation of China (31470785, 31000357), and the Zhejiang Province Science and Technology Plan (2014C32022).

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Received: 2016-8-2
Accepted: 2017-5-12
Published Online: 2017-12-29
Published in Print: 2017-11-27

© 2017 Institute of Botany, Slovak Academy of Sciences

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