Abstract
Zizania latifolia is a perennial aquatic vegetable of the Poaceae family. Chilling temperatures significantly affect stem enlargement and harvest time of Z. latifolia. Cold tolerance improves growth and harvest time at low temperatures. The study aimed to elucidate the proteomics and physiological differences between cold stress-tolerant (Longjiao 2) and -sensitive (911) Z. latifolia varieties. Superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) enzyme activities, as well as levels of malondialdehyde (MDA) and soluble sugars were determined. 2D gel electrophoresis and MOLDI-TOF MS were used to detect the differentially expressed proteins at normal and lower temperatures. Significant differences were observed for relative SOD, CAT, and POD enzyme activities, as well as for soluble sugar levels when comparing the two varieties grown at different temperatures. The results indicated that different responses of the two variety to chilling stress involved previously described physiological mechanisms. Proteomic changes under chilling stress suggested that key pathways including photosynthesis, energy metabolism, defense mechanisms, and RNA processing were differentially regulated in the chilling tolerant variety. Our findings provide a basis for the generation of new varieties Z. latifolia that could be capable of optimal growth under chilling stress, even in the context of global climate change.
Acknowledgements
This work was supported in part by the National Key Technology R&D Program (2016YFF0202300), the National Natural Science Foundation of China (31470785, 31000357), and the Zhejiang Province Science and Technology Plan (2014C32022).
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© 2017 Institute of Botany, Slovak Academy of Sciences
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Articles in the same Issue
- Zoology
- Identifying white spots on the roadmap of Late Pleistocene and Holocene palaeolimnology in Slovakia: Review and future directions
- Cellular and Molecular Biology
- Purification of small-size acidic proteoglycan-like domain of carbonic anhydrase IX fused with thioredoxine expressed in Escherichia coli for structural characterization
- Botany
- Functional and morphological traits of epiphytic lichens in the Western Carpathian oak forests reflect the influence of air quality and forest history
- Botany
- Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia
- Botany
- First signs of old-growth structure and composition of an oak forest after four decades of abandonment
- Botany
- Factors responsible for the distribution of invasive plant species in the surroundings of railway areas. A case study from SE Poland
- Botany
- The role of lipids and polysaccharides in model root mucilage with implications for the surface activity of the rhizosphere
- Botany
- Physiological and proteomic changes in Zizania latifolia under chilling stress
- Botany
- Novel polymorphic EST-based microsatellite markers characterized in lettuce (Lactuca sativa)
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- Identification of a HSP40 gene involved in planarian regeneration
- Zoology
- A new species of Bothropolys and a new record of Lithobius magnitergiferous (Lithobiidae) from the Qinghai-Tibet Plateau, China
- Zoology
- Myriapod (Chilopoda, Diplopoda) communities in hedgerows of upland agricultural landscape
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- Zoology
- Pre-winter larval activity and feeding behavior of Erebia aethiops and E. cassioides in Austrian Alps
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- Genetic structure of Apis mellifera carnica in Slovakia based on microsatellite DNA polymorphism
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- The growth inhibitory effects of garlic polysaccharide combined with cis-dichlorodiamine platinum on human HepG2 cells
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