Startseite Lebenswissenschaften Changes in expression of neurotrophins and neurotrophic factors in the model of eosinophilic inflammation of the esophageal mucosa
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Changes in expression of neurotrophins and neurotrophic factors in the model of eosinophilic inflammation of the esophageal mucosa

  • Mariana Brozmanová EMAIL logo , Jozef Hatok , Michal Hennel , Miloš Tatár und Andrea Vážanová
Veröffentlicht/Copyright: 29. Dezember 2017
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Biologia
Aus der Zeitschrift Biologia Band 72 Heft 11

Abstract

Esophageal sensory and motor nerves contribute to the symptoms of eosinophilic esophagitis, however, the mechanisms of this neural dysfunction are essentially unknown. We addressed the hypothesis that eosinophilic inflammation in the esophagus alters production of the key regulators of neural function neurotrophins and neurotrophic factors. We developed and optimized the model of allergic eosinophilic inflammation of esophageal mucosa induced by localized administration of allergen ovalbumin into the esophagus in ovalbumin-sensitized guinea pigs. We evaluated changes in expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF) and artemin in esophageal mucosa by quantitative RT-PCR. We found that the administration of ovalbumin into the esophageal wall of sensitized animals induced a massive eosinophilic infiltration restricted to esophageal mucosa (3 ± 1 vs. 97 ± 23 eosinophils per high power filed). This inflammatory response altered the expression profile of selected neurotrophic factors. The BDNF mRNA was increased (to 200%), artemin mRNA was decreased (to 50%) while NGF and GDNF was not changed. We conclude that a strong eosinophilic inflammation can be induced in guinea pigs and alters the expression of neurotrophins and neurotrophic factors. Our findings will aid mechanistic studies of neural dysfunction in eosinophilic esophagitis.

Key words: ovalbumin; eosinophilic esophagitis; eosinophils; neurotrophins; neurotrophic factors

Acknowledgements

This work was supported by grant Vega 1/0070/15 and by project “Biomedical Center Martin” ITMS code: 26220220 187, co-financed by EU sources.

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Received: 2017-6-14
Accepted: 2017-8-3
Published Online: 2017-12-29
Published in Print: 2017-11-27

© 2017 Institute of Zoology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2017-0140
Schlagwörter für diesen Artikel
ovalbumin; eosinophilic esophagitis; eosinophils; neurotrophins; neurotrophic factors

Artikel in diesem Heft

  1. Zoology
  2. Identifying white spots on the roadmap of Late Pleistocene and Holocene palaeolimnology in Slovakia: Review and future directions
  3. Cellular and Molecular Biology
  4. Purification of small-size acidic proteoglycan-like domain of carbonic anhydrase IX fused with thioredoxine expressed in Escherichia coli for structural characterization
  5. Botany
  6. Functional and morphological traits of epiphytic lichens in the Western Carpathian oak forests reflect the influence of air quality and forest history
  7. Botany
  8. Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia
  9. Botany
  10. First signs of old-growth structure and composition of an oak forest after four decades of abandonment
  11. Botany
  12. Factors responsible for the distribution of invasive plant species in the surroundings of railway areas. A case study from SE Poland
  13. Botany
  14. The role of lipids and polysaccharides in model root mucilage with implications for the surface activity of the rhizosphere
  15. Botany
  16. Physiological and proteomic changes in Zizania latifolia under chilling stress
  17. Botany
  18. Novel polymorphic EST-based microsatellite markers characterized in lettuce (Lactuca sativa)
  19. Cellular and Molecular Biology
  20. Identification of a HSP40 gene involved in planarian regeneration
  21. Zoology
  22. A new species of Bothropolys and a new record of Lithobius magnitergiferous (Lithobiidae) from the Qinghai-Tibet Plateau, China
  23. Zoology
  24. Myriapod (Chilopoda, Diplopoda) communities in hedgerows of upland agricultural landscape
  25. Zoology
  26. A new cockroach, with bipectinate antennae,(Blattaria: Olidae fam. nov.) further highlights the differences between the Burmite and other faunas
  27. Zoology
  28. Pre-winter larval activity and feeding behavior of Erebia aethiops and E. cassioides in Austrian Alps
  29. Zoology
  30. Genetic structure of Apis mellifera carnica in Slovakia based on microsatellite DNA polymorphism
  31. Zoology
  32. Amphibians in Czech zoological gardens — trends and implications for conservation
  33. Zoology
  34. Phenetic similarity of European golden jackal (Canis aureus moreoticus) populations from southeastern Europe based on craniometric data
  35. Celluar and Molecular Biology
  36. Fucoidan from Undaria pinnatifida regulates type II collagen and COX-2 expression via MAPK and PI3K pathways in rabbit articular chondrocytes
  37. Zoology
  38. Changes in expression of neurotrophins and neurotrophic factors in the model of eosinophilic inflammation of the esophageal mucosa
  39. Celluar and Molecular Biology
  40. The growth inhibitory effects of garlic polysaccharide combined with cis-dichlorodiamine platinum on human HepG2 cells
  41. Erratum
  42. Reptile surveys reveal high species richness in areas recovering from mining activity in the Brazilian Cerrado
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