Evaluation of the ABX Pentra 400: a newly available clinical chemistry analyser
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Pascal Coudène
Abstract
The performance of the ABX Pentra 400, a new multiparametric analyser available for routine and specialised clinical chemistry analyses, was evaluated according to the National Committee for Clinical Laboratory Standards (NCCLS) and Valtec protocols. Substrate concentrations and enzyme activities were determined by spectrophotometric measurement after coloured reaction or UV detection-based reactions; electrolyte concentrations were determined with ion-selective electrodes, and specific protein concentrations were assayed by immunoturbidimetry. In total, 32 of the most common clinical chemistry parameters were evaluated under simulated routine conditions. The analytical performance of the system and the quality of the ABX Pentra reagent line were both examined in a single-site study. The ABX Pentra 400 was compared with the Olympus AU640, Konelab-Konepro, Beckman Coulter-Immage and Beckman Coulter-Access 2, depending on the tests available on these analysers. The coefficient of variation (CV) values were within expected values. Coefficients of correlation showed high correlation between the analysers compared. The influence of interfering substances was moderate. The practicability of the system was good. We conclude that good reliability and practicability make the ABX Pentra 400 system suitable for laboratories with various needs.
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Artikel in diesem Heft
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- “Coelionomics”: towards understanding the molecular pathology of coeliac disease
- Gene expression profiling in human cardiovascular disease
- A common CYP1B1 polymorphism is associated with 2-OHE1/16-OHE1 urinary estrone ratio
- A quantitative RT-PCR method to determine topoisomerase I mRNA levels in human tissue samples
- Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy
- Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys ®
- Quantitative measurement of ketone bodies in urine using reflectometry
- Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry
- Determination of the antioxidant capacity in blood
- High-workload endurance training may increase serum ischemia-modified albumin concentrations
- Advanced oxidation protein products in serum of patients with myotonic disease type I: association with serum γ-glutamyltransferase and disease severity
- Effectiveness of different methods for anti-Sm antibody identification. A multicentre study
- Quality control in neutrophil granulocyte (PMN) concentrates by flow cytometry
- Circadian variation of holo-transcobalamin (holo-TC) and related markers
- Elevated levels of plasma homocysteine in postmenopausal women in Burkina Faso
- Allergy testing on the IMMULITE 2000 Random-Access immunoanalyzer – a clinical evaluation study
- Evaluation of the ABX Pentra 400: a newly available clinical chemistry analyser
