Effectiveness of different methods for anti-Sm antibody identification. A multicentre study
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María Jesús Llorente
Abstract
Methods for the measurement of autoantibodies frequently provide controversial results. The objective of the present study was to evaluate the performance of Spanish Clinical Laboratories in the measurement of anti-Sm antibodies. A total of 23 laboratories participated, analysing 30 serum samples from patients with systemic lupus erythematosus and other autoimmune and non-autoimmune diseases. The laboratories used four extractable nuclear antigen screens, eight enzyme-linked immunosorbent assays (ELISAs) specific for anti-Sm, one line-blot, one dot-blot and one double immunodiffusion assay, from 15 different manufacturers. A total of 871 results were obtained. In general, very good sensitivity was obtained (95–100%), but specificity was moderate (52–86%) and must be improved. Most ELISAs and the line-blot were valid assays for anti-Sm detection and could serve as tests both for analysis and/or confirmation. The likelihood ratios indicated that both methods can be considered very useful or useful for the determination of anti-Sm antibodies. Nevertheless, the analytical quality of the methods for the measurement of anti-Sm antibodies could probably be improved by standardisation of the methods and the participation of laboratories in external quality control programs.
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©2005 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Inter-individual variation of inflammatory markers of cardiovascular risks and diseases
- “Coelionomics”: towards understanding the molecular pathology of coeliac disease
- Gene expression profiling in human cardiovascular disease
- A common CYP1B1 polymorphism is associated with 2-OHE1/16-OHE1 urinary estrone ratio
- A quantitative RT-PCR method to determine topoisomerase I mRNA levels in human tissue samples
- Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy
- Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys ®
- Quantitative measurement of ketone bodies in urine using reflectometry
- Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry
- Determination of the antioxidant capacity in blood
- High-workload endurance training may increase serum ischemia-modified albumin concentrations
- Advanced oxidation protein products in serum of patients with myotonic disease type I: association with serum γ-glutamyltransferase and disease severity
- Effectiveness of different methods for anti-Sm antibody identification. A multicentre study
- Quality control in neutrophil granulocyte (PMN) concentrates by flow cytometry
- Circadian variation of holo-transcobalamin (holo-TC) and related markers
- Elevated levels of plasma homocysteine in postmenopausal women in Burkina Faso
- Allergy testing on the IMMULITE 2000 Random-Access immunoanalyzer – a clinical evaluation study
- Evaluation of the ABX Pentra 400: a newly available clinical chemistry analyser
