Linearity and detection limit in the measurement of serum M-protein with the capillary zone electrophoresis system Capillarys ®
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Enrique Bergón
Abstract
We studied the linearity and detection limits of the capillary zone electrophoresis system Capillarys ® in the measurement of serum monoclonal protein. Three monoclonal proteins with different isotypes and electrophoretic migrations were diluted with a hypo-γ-globulinemic polyclonal serum pool. Mathematical linearity was observed for all monoclonal protein isotypes in the ranges studied without removing the polyclonal γ-globulin background. Theoretical concentrations of 0.43, 0.89 and 0.33g/L for monoclonal proteins immunoglobulin (Ig)G, IgA and IgM, respectively, gave a discernible spike by Capillarys ®, although they were measured as 0.76, 1.09 and 0.76g/L, respectively. We observed overestimation of monoclonal protein inversely correlated to concentrations below 15g/L. All these limitations have to be taken into account when monitoring monoclonal proteins, because the loss of linearity and the protein background may hide an increase in concentration at low levels.
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©2005 by Walter de Gruyter Berlin New York
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Articles in the same Issue
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- Quantitative measurement of ketone bodies in urine using reflectometry
- Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry
- Determination of the antioxidant capacity in blood
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- Advanced oxidation protein products in serum of patients with myotonic disease type I: association with serum γ-glutamyltransferase and disease severity
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- Allergy testing on the IMMULITE 2000 Random-Access immunoanalyzer – a clinical evaluation study
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