Small-angle X-ray and neutron scattering as a tool for structural systems biology
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Dmitri I. Svergun
Abstract
Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a few kDa to hundreds of MDa) and experimental conditions (temperature, pH, salinity, ligand addition, etc.), which is of primary importance for a systemic approach in structural biology. The method provides unique information about the overall structure and conformational changes of native individual proteins, functional complexes, flexible macromolecules and hierarchical systems. New developments in small-angle X-ray and neutron scattering studies of biological macromolecules in solution are briefly reviewed, with a special emphasis on technical and methodological approaches useful for structural systems biology. Possibilities of synergistic use of the method with other techniques are considered.
©2010 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- Guest Editorial
- Highlight: Of Systems and Structures
- HIGHLIGHT: STRUCTURAL SYSTEMS BIOLOGY
- Converging on the function of intrinsically disordered nucleoporins in the nuclear pore complex
- Towards molecular systems biology of gene transcription and regulation
- Small-angle X-ray and neutron scattering as a tool for structural systems biology
- The type III secretion injectisome, a complex nanomachine for intracellular ‘toxin’ delivery
- Structural insights into the evolution of the adaptive immune system: the variable lymphocyte receptors of jawless vertebrates
- The XPD helicase: XPanDing archaeal XPD structures to get a grip on human DNA repair
- Decoding transcription and microRNA-mediated translation control in Drosophila development
- Human SepSecS or SLA/LP: selenocysteine formation and autoimmune hepatitis
- PROTEIN STRUCTURE AND FUNCTION
- The rhodanese RhdA helps Azotobacter vinelandii in maintaining cellular redox balance
- MEMBRANES, LIPIDS, GLYCOBIOLOGY
- The membrane-bound bile acid receptor TGR5 (Gpbar-1) is localized in the primary cilium of cholangiocytes
- CELL BIOLOGY AND SIGNALING
- miR-221/222 suppression protects against endoplasmic reticulum stress-induced apoptosis via p27Kip1- and MEK/ERK-mediated cell cycle regulation
- Tissue kallikrein promotes prostate cancer cell migration and invasion via a protease-activated receptor-1-dependent signaling pathway
- Sprouty4 levels are increased under hypoxic conditions by enhanced mRNA stability and transcription
- PROTEOLYSIS
- Degradation of human kininogens with the release of kinin peptides by extracellular proteinases of Candida spp.
- NOVEL TECHNIQUES
- Detection of breast cancer-related antigens through cDNA phage-displayed protein microarray
Artikel in diesem Heft
- Guest Editorial
- Highlight: Of Systems and Structures
- HIGHLIGHT: STRUCTURAL SYSTEMS BIOLOGY
- Converging on the function of intrinsically disordered nucleoporins in the nuclear pore complex
- Towards molecular systems biology of gene transcription and regulation
- Small-angle X-ray and neutron scattering as a tool for structural systems biology
- The type III secretion injectisome, a complex nanomachine for intracellular ‘toxin’ delivery
- Structural insights into the evolution of the adaptive immune system: the variable lymphocyte receptors of jawless vertebrates
- The XPD helicase: XPanDing archaeal XPD structures to get a grip on human DNA repair
- Decoding transcription and microRNA-mediated translation control in Drosophila development
- Human SepSecS or SLA/LP: selenocysteine formation and autoimmune hepatitis
- PROTEIN STRUCTURE AND FUNCTION
- The rhodanese RhdA helps Azotobacter vinelandii in maintaining cellular redox balance
- MEMBRANES, LIPIDS, GLYCOBIOLOGY
- The membrane-bound bile acid receptor TGR5 (Gpbar-1) is localized in the primary cilium of cholangiocytes
- CELL BIOLOGY AND SIGNALING
- miR-221/222 suppression protects against endoplasmic reticulum stress-induced apoptosis via p27Kip1- and MEK/ERK-mediated cell cycle regulation
- Tissue kallikrein promotes prostate cancer cell migration and invasion via a protease-activated receptor-1-dependent signaling pathway
- Sprouty4 levels are increased under hypoxic conditions by enhanced mRNA stability and transcription
- PROTEOLYSIS
- Degradation of human kininogens with the release of kinin peptides by extracellular proteinases of Candida spp.
- NOVEL TECHNIQUES
- Detection of breast cancer-related antigens through cDNA phage-displayed protein microarray
