Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
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M. P. Rudolf
Abstract
It has been postulated that upon binding to a cell surface receptor, papilloma virus-like particles (VLPs) gain entry into the cytosol of infected cells and the capsid proteins L1 and L2 can be processed in the MHC class I presentation pathway. Vaccination of mice with human papilloma virus-like particles consisting of capsid proteins L1 and L2 induced a CD8- mediated and perforin dependent protective immune response against a tumor challenge with human papilloma virus transformed tumor cells, which express only minute amounts of L1 protein. Here we show that HPV16 capsid proteins stimulate a MHC class I restricted CTL response with human peripheral blood lymphocytes (PBL) in vitro. The vigorous response was specific for VLP-infected target cells and was MHC class I restricted. Moreover we show the presence of at least one HLA-A*0201 restricted CTL epitope within the HPV-16 capsid proteins by using a VLP-‘infected’ HLA-A*0201 transfected human cell line as target cells. These results demonstrated that VLPs can induce a HPV16 capsid protein-specific immune response in humans, allowing the monitoring of immune responses induced by vaccines based on chimeric VLPs carrying additional immunogenic peptides or proteins in therapeutical applications in human patients.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Chimeric Virus-Like Particles as Vaccines
- The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
- Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
- The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
- Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
- Chaperones Involved in Hepatitis B Virus Morphogenesis
- Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
- HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
- Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
- Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
- Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
- A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
- DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
- Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
- Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
- Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
- Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
