Article
Licensed
Unlicensed
Requires Authentication
Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
-
U. Schmidt
Published/Copyright:
June 1, 2005
Abstract
For the development of gene therapy protocols based on polyomavirus-like particles, we describe a method for fluorescence labelling of virions in order to study virus-cell interactions preceding gene delivery. Site-specific fluorescence labelling of polyomavirus-like particles is achieved via a single cysteine residue and maleimide conjugates of fluorescence dyes (fluorescein, Texas Red). Polyomavirus-like particles can be assembled in vitro from recombinant capsomers produced in E. coli. Since the assembly process is independent of disulfide bond formation, all cysteine residues of the wild-type protein were replaced by serines, and a new unique cysteine residue was introduced for the attachment of the fluorescence marker.
:
Published Online: 2005-06-01
Published in Print: 1999-03-01
Copyright (c) 1999 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Chimeric Virus-Like Particles as Vaccines
- The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
- Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
- The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
- Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
- Chaperones Involved in Hepatitis B Virus Morphogenesis
- Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
- HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
- Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
- Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
- Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
- A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
- DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
- Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
- Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
- Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
- Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
Articles in the same Issue
- Chimeric Virus-Like Particles as Vaccines
- The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
- Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
- The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
- Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
- Chaperones Involved in Hepatitis B Virus Morphogenesis
- Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
- HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
- Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
- Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
- Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
- A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
- DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
- Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
- Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
- Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
- Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
