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Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 380 Issue 3

Abstract

Polyomavirus-derived virus-like particles (VLPs) have been described as potential carriers for encapsidation of nucleic acids in gene therapy. Although VLPs can be generated in E. coli or insect cells, the yeast expression system should be advantageous as it is well established for the biotechnological generation of products for human use, especially because they are free of toxins hazardous for humans. We selected the yeast Saccharomyces cerevisiae for expression of the major capsid protein VP1 of a non-human polyomavirus, the hamster polyomavirus (HaPV). Two entire HaPV VP1- coding sequences, starting with the authentic and a second upstream ATG, respectively, were subcloned and expressed to high levels in Saccharomyces cerevisiae. The expressed VP1 assembled spontaneously into VLPs with a structure resembling that of the native HaPV capsid. Determination of the subcellular localization revealed a nuclear localization of some particles formed by the N-terminally extended VP1, whereas particles formed by the authentic VP1 were found mainly in the cytoplasmic compartment.

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Published Online: 2005-06-01
Published in Print: 1999-03-01

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Chimeric Virus-Like Particles as Vaccines
  2. The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
  3. Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
  4. The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
  5. Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
  6. Chaperones Involved in Hepatitis B Virus Morphogenesis
  7. Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
  8. HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
  9. Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
  10. Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
  11. Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
  12. A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
  13. DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
  14. Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
  15. Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
  16. Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
  17. Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
https://doi.org/10.1515/BC.1999.050

Articles in the same Issue

  1. Chimeric Virus-Like Particles as Vaccines
  2. The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
  3. Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
  4. The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
  5. Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
  6. Chaperones Involved in Hepatitis B Virus Morphogenesis
  7. Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
  8. HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
  9. Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
  10. Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
  11. Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
  12. A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
  13. DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
  14. Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
  15. Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
  16. Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
  17. Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
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