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HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 380 Issue 3

Abstract

Core particles of the hepatitis B virus (HBV) potentiate the immune response against foreign epitopes presented on their surface. Potential insertion sites in the monomeric subunit of the HBV core protein were previously identified at the N- and C-terminus and in the immunodominant c/e1 region. In a C-terminally truncated core protein these sites were used to introduce the entire 120 amino acid (aa)-long potentially immunoprotective region of the hantavirus (serotype Puumala) nucleocapsid protein. The N- and C-terminal fusion products were unable to form core-like particles in detectable amounts. However, a suppressable stop codon located between the HBV core and the C-terminally fused hantavirus sequence restored the ability to form particles (‘mosaic particles’); in contrast to the C-terminal fusion product the mosaic construct allowed the formation of particles built up by the core protein itself and the HBV core-Puumala nucleocapsid- readthrough protein. The mosaic particles exposed the 120 aa region of the PUU nucleocapsid protein on their surface as demonstrated by ELISA and immuno electron microscopy applying different monoclonal antibodies. Insertion of the hantaviral sequence into the c/e1 region not only allowed the formation of chimeric particles, but again the surface accessibility of the sequence. HBV core antigenicity itself was, however, reduced in the particles carrying insertions in the c/e1 region, probably due to a masking effect of the 120 aa long insert.

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Published Online: 2005-06-01
Published in Print: 1999-03-01

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Chimeric Virus-Like Particles as Vaccines
  2. The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
  3. Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
  4. The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
  5. Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
  6. Chaperones Involved in Hepatitis B Virus Morphogenesis
  7. Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
  8. HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
  9. Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
  10. Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
  11. Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
  12. A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
  13. DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
  14. Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
  15. Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
  16. Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
  17. Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
https://doi.org/10.1515/BC.1999.044

Articles in the same Issue

  1. Chimeric Virus-Like Particles as Vaccines
  2. The Core Antigen of Hepatitis B Virus as a Carrier for Immunogenic Peptides
  3. Enhancing the Immunogenicity of Exogenous Hepatitis B Surface Antigen-Based Vaccines for MHC-I-Restricted T Cells
  4. The Role of the Proteasome System and the Proteasome Activator PA28 Complex in the Cellular Immune Response
  5. Ty Virus-Like Particles, DNA Vaccines and Modified Vaccinia Virus Ankara; Comparisons and Combinations
  6. Chaperones Involved in Hepatitis B Virus Morphogenesis
  7. Behavior of a Short preS1 Epitope on the Surface of Hepatitis B Core Particles
  8. HBV Core Particles Allow the Insertion and Surface Exposure of the Entire Potentially Protective Region of Puumala Hantavirus Nucleocapsid Protein
  9. Induction of HPV16 Capsid Protein-Specific Human T Cell Responses by Virus-Like Particles
  10. Construction and Characterization of Recombinant VLPs and Semliki-Forest Virus Live Vectors for Comparative Evaluation in the SHIV Monkey Model
  11. Development of HIV/AIDS Vaccine Using Chimeric gag-env Virus-Like Particles
  12. A Disulfide-Bound HIV-1 V3 Loop Sequence on the Surface of Human Rhinovirus 14 Induces Neutralizing Responses against HIV-1
  13. DNA-Plasmids of HIV-1 Induce Systemic and Mucosal Immune Responses
  14. Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles
  15. Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus
  16. Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1
  17. Site-Specific Fluorescence Labelling of Recombinant Polyomavirus-Like Particles
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