Implication of caffeine consumption and recovery on the reproductive functions of adult male Wistar rats
-
Omobola F. Oluwole
,
Eunice Ogunwole
Abstract
Background:
This study assessed the impact of caffeine consumption and recovery on reproductive functions and fertility of Wistar rats.
Methods:
Thirty-five adult male Wistar rats were divided into seven groups of five rats each. Group A (control) received distilled water (vehicle), while groups B, C, and D were treated orally with 10 mg/kg body weight (BW), 20 mg/kg BW, and 40 mg/kg BW caffeine, respectively, for 30 days. Groups E, F, and G were treated orally with 10 mg/kg BW, 20 mg/kg BW, and 40 mg/kg BW caffeine, respectively, for 30 days and then allowed to recover for another 30 days.
Results:
Caffeine caused a decrease in body weight, while recovery groups showed appreciable increase in body weight during recovery. Relative weight of seminal vesicle, prostate, and epididymis decreased dose dependently during treatment but increased during recovery. The liver and kidney weight increased during treatment but reduced during recovery. Sperm count was significantly decreased in both treated and recovery groups. Initial decrease in sperm viability and volume was appreciably reversed during recovery period. Serum level of testosterone increased at high doses, while serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) showed significant decrease. Histological sections of testis in treated groups showed mild congestion of the interstitial blood vessel and subcapsular congestion. However, there was no subcapsular congestion in the recovery groups. All rats in both treated and recovery groups had 100% fertilization success from fertility study.
Conclusions:
Suggestively, caffeine treatment for 4 weeks could impair body, reproductive organs weight, sperm characteristics, LH/FSH level, and also testicular cyto-architecture. Effects appeared, however, reversible after caffeine withdrawal.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
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- Anxiolytic-like effect of the leaves of Pseudospondias microcarpa (A. Rich.) Engl. in mice
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Articles in the same Issue
- Frontmatter
- Review
- Non-diabetic clinical applications of insulin
- Mini Review
- Monophasic action potential recordings: which is the recording electrode?
- Behavior and Neuroprotection
- Histamine H3 receptor antagonists display antischizophrenic activities in rats treated with MK-801
- Nephroprotective effect of β-sitosterol on N-diethylnitrosamine initiated and ferric nitrilotriacetate promoted acute nephrotoxicity in Wistar rats
- Reproduction
- Implication of caffeine consumption and recovery on the reproductive functions of adult male Wistar rats
- Cardiovascular Function
- Cosmic ray (neutron) activity and air pollution nanoparticles – cardiovascular disease risk factors – separate or together?
- Oxidative Stress
- Nephrotoxicity of sodium valproate and protective role of L-cysteine in rats at biochemical and histological levels
- Effect of protocatechuic acid on lipid profile and DNA damage in D-galactosamine-induced hepatotoxic rats
- Oral administration of green plant-derived chemicals and antioxidants alleviates stress-induced cellular oxidative challenge
- Phytotherapy
- Scientific evidence of plant with a rapid-onset and sustained antidepressant effect in a chronic model of depression: Mallotus oppositifolius
- Anxiolytic-like effect of the leaves of Pseudospondias microcarpa (A. Rich.) Engl. in mice
- Comparative phytochemical profiling of Clerodendrum infortunatum with some selected medicinal plants predominant in the Sub-Himalayan region of West Bengal
