Oral administration of green plant-derived chemicals and antioxidants alleviates stress-induced cellular oxidative challenge

Elizabeth A. Beaven; Kay L. Colthorpe; Jereme G. Spiers; Hsiao-Jou Cortina Chen; Nickolas A. Lavidis; Julie Albrecht

doi:10.1515/jbcpp-2016-0006

Article

Oral administration of green plant-derived chemicals and antioxidants alleviates stress-induced cellular oxidative challenge

Elizabeth A. Beaven , Kay L. Colthorpe , Jereme G. Spiers , Hsiao-Jou Cortina Chen , Nickolas A. Lavidis and Julie Albrecht

Published/Copyright: May 16, 2016

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 27 Issue 5

Abstract

Background:

This study examined the efficacy of the combination antioxidant, Formula 42 (F42), on cellular stress indicators in animal and human models of stress-induced oxidative stress.

Methods:

A sub-chronic psychological stress model in rodents was used to induce stress and oxidative stress indicators over a 10-day period during which animals received oral doses of F42 or water. Following treatment, body weight, plasma stress hormone corticosterone, and oxidative capacity were evaluated. In healthy human subjects, a randomized double-blind crossover study was used to examine the antioxidant effect of F42 or placebo in an exercise-induced oxidative stress model. Erythrocyte and plasma oxidative status was evaluated using the fluorescent activation of 2′,7′-dichlorofluorescin (DCF) as an indicator.

Results:

Oral administration of F42 reduced the corticosterone response to acute stress compared to vehicle but did not differ at the conclusion of the 10-day study. However, F42 administration did reduce stress-induced growth restriction and alleviate DCF activation in circulating erythrocytes by approximately 10% following 10 days of stress exposure. Oral administration of F42 also significantly reduced DCF activation by approximately 10% in healthy human subjects undergoing exercise-induced oxidative stress.

Conclusions:

Oral administration of F42 in rodents produces transient reductions in stress hormones and reduces stress indicators following sub-chronic psychological stress exposure. In humans, F42 acts as an early and potent antioxidant capable of scavenging free radicals within 30 min of ingestion.

Keywords: oxidative stress; physical exercise; plant-derived chemicals; reactive oxygen species; stress

Acknowledgments

We would like to thank Ms. Erica W. H. Mu and Ms. Jessica M. Soden for their assistance in animal treatment and the animal technicians from the University of Queensland Biological Resources based at Herston. We would also like to acknowledge Mr. Stephen Moss, Mr. Lindsay Shannon, Ms. Elizabeth McLennan, and Ms. Lien Banh for their assistance with the human studies.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by a University of Queensland Research Grant (Brisbane, QLD, Australia) and Leopa BioHealth Pty. Ltd. (Annerley, QLD, Australia).
Employment or leadership: J.A. and N.A.L. are unpaid consultants to Leopa BioHealth Pty. Ltd. The remaining authors declare no competing financial interests.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2016-1-11

Accepted: 2016-4-26

Published Online: 2016-5-16

Published in Print: 2016-9-1

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2016-0006

Keywords for this article

oxidative stress; physical exercise; plant-derived chemicals; reactive oxygen species; stress