Effect of protocatechuic acid on lipid profile and DNA damage in D-galactosamine-induced hepatotoxic rats
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Thangaiyan Radhiga
Abstract
Background:
Our aim in this study is to investigate the effect of protocatechuic acid (PCA) on lipid profile and DNA damage in D-galactosamine (D-GalN)-induced hepatotoxic rats.
Methods:
Hepatotoxicity was induced by a single intraperitoneal dose of D-GalN in male Wistar rats. The activities of hepatic markers and levels of kidney function markers were determined. The plasma and tissue lipid levels were estimated. DNA damage was determined by COMET assay. Histopathological examination was also performed using portions of the liver and kidney tissues.
Results:
D-GalN-induced hepatotoxic rats showed increased in the activities of hepatic marker enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and γ-glutamyl transpeptidase (GGT) in serum. The levels of kidney function markers such as urea, uric acid, and creatinine increased in serum. Levels of lipid profile such as total cholesterol (TC), triglycerides (TG), free fatty acid (FFA), and phospholipids (PLs) in the plasma and tissues (liver and kidney) were significantly increased in D-GalN-induced rats. In plasma, levels of very low density lipoprotein cholesterol (VLDL-C) and low-density lipoprotein cholesterol (LDL-C) significantly increased, whereas high-density lipoprotein cholesterol (HDL-C) level decreased in D-GalN-induced rats. Furthermore, D-GalN-induced rats showed increased percentage of tail DNA and tail length and decreased percentage of head DNA. Oral administration of PCA (100 mg/ kg BW) for 20 days improved these levels when compared to D-GalN-induced rats. These biochemical changes were reflected on the attenuation and the structural alteration of the liver and kidney integrity.
Conclusions:
The results of the study suggest that PCA has a potent hepatoprotective activity that may be linked to its antihyperlipidemic effect.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review
- Non-diabetic clinical applications of insulin
- Mini Review
- Monophasic action potential recordings: which is the recording electrode?
- Behavior and Neuroprotection
- Histamine H3 receptor antagonists display antischizophrenic activities in rats treated with MK-801
- Nephroprotective effect of β-sitosterol on N-diethylnitrosamine initiated and ferric nitrilotriacetate promoted acute nephrotoxicity in Wistar rats
- Reproduction
- Implication of caffeine consumption and recovery on the reproductive functions of adult male Wistar rats
- Cardiovascular Function
- Cosmic ray (neutron) activity and air pollution nanoparticles – cardiovascular disease risk factors – separate or together?
- Oxidative Stress
- Nephrotoxicity of sodium valproate and protective role of L-cysteine in rats at biochemical and histological levels
- Effect of protocatechuic acid on lipid profile and DNA damage in D-galactosamine-induced hepatotoxic rats
- Oral administration of green plant-derived chemicals and antioxidants alleviates stress-induced cellular oxidative challenge
- Phytotherapy
- Scientific evidence of plant with a rapid-onset and sustained antidepressant effect in a chronic model of depression: Mallotus oppositifolius
- Anxiolytic-like effect of the leaves of Pseudospondias microcarpa (A. Rich.) Engl. in mice
- Comparative phytochemical profiling of Clerodendrum infortunatum with some selected medicinal plants predominant in the Sub-Himalayan region of West Bengal
Artikel in diesem Heft
- Frontmatter
- Review
- Non-diabetic clinical applications of insulin
- Mini Review
- Monophasic action potential recordings: which is the recording electrode?
- Behavior and Neuroprotection
- Histamine H3 receptor antagonists display antischizophrenic activities in rats treated with MK-801
- Nephroprotective effect of β-sitosterol on N-diethylnitrosamine initiated and ferric nitrilotriacetate promoted acute nephrotoxicity in Wistar rats
- Reproduction
- Implication of caffeine consumption and recovery on the reproductive functions of adult male Wistar rats
- Cardiovascular Function
- Cosmic ray (neutron) activity and air pollution nanoparticles – cardiovascular disease risk factors – separate or together?
- Oxidative Stress
- Nephrotoxicity of sodium valproate and protective role of L-cysteine in rats at biochemical and histological levels
- Effect of protocatechuic acid on lipid profile and DNA damage in D-galactosamine-induced hepatotoxic rats
- Oral administration of green plant-derived chemicals and antioxidants alleviates stress-induced cellular oxidative challenge
- Phytotherapy
- Scientific evidence of plant with a rapid-onset and sustained antidepressant effect in a chronic model of depression: Mallotus oppositifolius
- Anxiolytic-like effect of the leaves of Pseudospondias microcarpa (A. Rich.) Engl. in mice
- Comparative phytochemical profiling of Clerodendrum infortunatum with some selected medicinal plants predominant in the Sub-Himalayan region of West Bengal
