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Gelatinase matrix metalloproteinase (MMP)-2 and MMP-9 of the umbilical cord blood in preeclampsia

  • Zofia Galewska , Lech Romanowicz , Stefan Jaworski and Edward Bańkowski
Published/Copyright: February 26, 2008
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 46 Issue 4

Abstract

Background: Preeclampsia is associated with accumulation of collagen and proteoglycans in the umbilical cord tissues as a result of increased biosynthesis and decreased degradation of these components. Matrix metalloproteinases (MMPs) are enzymes engaged in the degradation of collagen and the protein core structures of proteoglycans, including those which bind peptide growth factors.

Methods: We used Western immunoblots, immunoenzymatic assay (ELISA) and zymography techniques for the detection of gelatinases and their inhibitors.

Results: We found that both umbilical cord blood plasma and serum of controls and preeclamptic newborns contained MMP-2 and MMP-9, as well tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2. The umbilical cord plasma of preeclamptic subjects contained large amounts of MMP-9 in a form of complexes with other plasma components, and zymographic analysis demonstrated increased gelatinolytic activity at a position corresponding to MMP-9, compared to control samples. By contrast, MMP-2, TIMP-1 and TIMP-2 data showed no significant differences between preeclamptic and control samples.

Conclusions: The high activity of MMP-9 in preeclamptic plasma suggests its participation in the proteolytic release of peptide growth factors from their complexes with other matrix components, with subsequent stimulation of cell division and matrix biosynthesis. We suggest this might represent one of the mechanisms for matrix remodeling in the umbilical cord of preeclamptic newborns.

Clin Chem Lab Med 2008;46:517–22.

Keywords: gelatinases; metalloproteinases; preeclampsia; umbilical blood plasma; umbilical blood serum
Corresponding author: Edward Bańkowski, MD, DSc, Department of Medical Biochemistry, Medical Academy of Białystok, ul. Mickiewicza 2, 15-089 Białystok, Poland Phone: +48-85-7485577, Fax: +48-85-7485578,
Received: 2007-10-4
Accepted: 2007-11-27
Published Online: 2008-02-26
Published in Print: 2008-04-01

©2008 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2008.083
Keywords for this article
gelatinases; metalloproteinases; preeclampsia; umbilical blood plasma; umbilical blood serum

Articles in the same Issue

  1. Editorial
  2. Improving clinicians understanding of lipoprotein sub-fractions through new technology
  3. Review
  4. Free fatty acids as a cardiovascular risk factor
  5. Opinion Papers
  6. Pharmacy-based laboratory services: past or future and risk or opportunity?
  7. Standards of practice and uniformity in references style
  8. Genetics and Molecular Diagnostics
  9. Glutathione S-transferase variants increase susceptibility for late-onset Alzheimer's disease: association study and relationship with apolipoprotein E ɛ4 allele
  10. The D allele of angiotensin I-converting enzyme gene insertion/deletion polymorphism is associated with the severity of atherosclerosis
  11. The apolipoprotein A5 gene –1131T→C polymorphism affects vitamin E plasma concentrations in type 2 diabetic patients
  12. Cell lines that express membrane-associated DNA for anti-DNA antibody detection
  13. Certification of reference materials for detection of the human prothrombin gene G20210A sequence variant
  14. Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?
  15. Performance characteristics of a real-time RT-PCR assay for quantification of hepatitis C virus RNA in patients with genotype 1 and 2 infections
  16. Apolipoprotein E polymorphisms in Mexican patients with coronary artery disease
  17. A new single nucleotide polymorphism genotyping method based on gap ligase chain reaction and a microsphere detection assay
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  19. PROCAM Study: risk prediction for myocardial infarction using microfluidic high-density lipoprotein (HDL) subfractionation is independent of HDL cholesterol
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  23. Gelatinase matrix metalloproteinase (MMP)-2 and MMP-9 of the umbilical cord blood in preeclampsia
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  25. Carbonylation of platelet proteins occurs as consequence of oxidative stress and thrombin activation, and is stimulated by ageing and type 2 diabetes
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  27. Non-high-density lipoprotein cholesterol levels and recent involuntary weight gain among asymptomatic female subjects
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  30. Hematological parameters in preterm infants from birth to 16 weeks of age with reference to iron balance
  31. Influence of hydroxyethyl starch (6% HES 130/0.4) administration on hematology and clinical chemistry parameters
  32. Letters to the Editor
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