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Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?
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Yun Lu
Published/Copyright:
February 26, 2008
Abstract
Background: The aim of this study was to detect the expression of apoptosis factor caspase-3 in transferred HepG2 cells and provide feasible evaluation of the treatment for primary liver cancer with gene methods.
Methods: The pcDNA4C-LIGHT cDNA was extracted from Escherichia coli JM-109; then, the pcDNA4C-LIGHT cDNA was transferred into the HepG2 cells by a cationic liposome mediated method. Meanwhile, the blank group was established as the control group and the HepG2 cells were collected after transfection at 12 h, 24 h, 48 h, 3 days and 5 days. The expression of caspase-3 was identified in the supernatants by ELISA. A standard curve was generated for the set of samples assayed. Statistical significance was analyzed by SPSS.
Results: The quantity of caspase-3 protein was the greatest at 48 h and the least on day 5. The secretion of caspase-3 did not increase in the control group. The coefficient of correlation was equal to 0.9986 and had evident significance.
Conclusions: The pcDNA4C-LIGHT was effectively transfected in human HepG2 cells mediated by liposome. The expression of caspase-3 increased in the transfected group. This study provides necessary theoretic support for the treatment of liver cancer with gene methods.
Clin Chem Lab Med 2008;46:470–4.
Received: 2007-10-20
Accepted: 2007-11-30
Published Online: 2008-02-26
Published in Print: 2008-04-01
©2008 by Walter de Gruyter Berlin New York
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