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Cell lines that express membrane-associated DNA for anti-DNA antibody detection

  • Elio Tonutti , Nadia Blasone , Daniela Visentini , Monica Poletto , Danilo Villalta , Renato Tozzoli and Nicola Bizzaro
Published/Copyright: April 1, 2008
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 46 Issue 4

Abstract

Background: In this study, we evaluated the analytical reliability and clinical sensitivity of two tests (indirect immunofluorescence and ELISA) for anti-DNA antibodies which use cell membrane DNA (cmDNA) as antigen (Wil2 NS lymphoblastoid B cell line).

Methods: We tested 97 sera of patients with systemic lupus erythematosus (SLE) and 140 control sera from healthy subjects or from patients with other systemic autoimmune diseases or infectious diseases. The results obtained with the two anti-cmDNA kits were compared with those obtained with three other methods: indirect immunofluorescence on Crithidia luciliae, ELISA anti-double-stranded DNA (anti-dsDNA) and ELISA anti-nucleosome.

Results: The diagnostic sensitivity was 85% for immunofluorescence cmDNA and 90% for ELISA cmDNA, i.e., much higher than that of Crithidia (51%) and ELISA dsDNA (71%) methods, and the same as that of the ELISA nucleosome test (85%). The specificity of the cmDNA tests proved lower (92% for immunofluorescence and 87% for ELISA) than that found by anti-dsDNA Crithidia (99%), ELISA (99%) and anti-nucleosome tests (100%).

Conclusions: The results of our study indicate that cmDNA tests may be used as screening tests in patients with suspected SLE. The anti-nucleosome test offers the best diagnostic accuracy overall and can play an important role in the serological diagnosis of SLE.

Clin Chem Lab Med 2008;46:458–62.

Keywords: anti-double-stranded DNA (anti-dsDNA); anti-nucleosome; cell membrane DNA; ELISA; indirect immunofluorescence; systemic lupus erythematosus
Corresponding author: Dr. Elio Tonutti, MD, Laboratorio di Immunopatologia e Allergologia, Azienda Ospedaliero-Universitaria S. Maria della Misericordia, P.le S. Maria della Misericordia, 33100 Udine, Italy Phone: +39-0432-552403, Fax: +39-0432-552402,
Received: 2007-9-28
Accepted: 2007-12-17
Published Online: 2008-04-01
Published in Print: 2008-04-01

©2008 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2008.105
Keywords for this article
anti-double-stranded DNA (anti-dsDNA); anti-nucleosome; cell membrane DNA; ELISA; indirect immunofluorescence; systemic lupus erythematosus

Articles in the same Issue

  1. Editorial
  2. Improving clinicians understanding of lipoprotein sub-fractions through new technology
  3. Review
  4. Free fatty acids as a cardiovascular risk factor
  5. Opinion Papers
  6. Pharmacy-based laboratory services: past or future and risk or opportunity?
  7. Standards of practice and uniformity in references style
  8. Genetics and Molecular Diagnostics
  9. Glutathione S-transferase variants increase susceptibility for late-onset Alzheimer's disease: association study and relationship with apolipoprotein E ɛ4 allele
  10. The D allele of angiotensin I-converting enzyme gene insertion/deletion polymorphism is associated with the severity of atherosclerosis
  11. The apolipoprotein A5 gene –1131T→C polymorphism affects vitamin E plasma concentrations in type 2 diabetic patients
  12. Cell lines that express membrane-associated DNA for anti-DNA antibody detection
  13. Certification of reference materials for detection of the human prothrombin gene G20210A sequence variant
  14. Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?
  15. Performance characteristics of a real-time RT-PCR assay for quantification of hepatitis C virus RNA in patients with genotype 1 and 2 infections
  16. Apolipoprotein E polymorphisms in Mexican patients with coronary artery disease
  17. A new single nucleotide polymorphism genotyping method based on gap ligase chain reaction and a microsphere detection assay
  18. General Clinical Chemistry and Laboratory Medicine
  19. PROCAM Study: risk prediction for myocardial infarction using microfluidic high-density lipoprotein (HDL) subfractionation is independent of HDL cholesterol
  20. Evaluation of oxidative stress and inflammation in obese adults with metabolic syndrome
  21. Sex differences in adenosine deaminase activity of stroke patients
  22. Serum levels of the osteoprotegerin, receptor activator of nuclear factor κ-B ligand, metalloproteinase-1 (MMP-1) and tissue inhibitors of MMP-1 levels are increased in men 6 months after acute myocardial infarction
  23. Gelatinase matrix metalloproteinase (MMP)-2 and MMP-9 of the umbilical cord blood in preeclampsia
  24. Association between plasma alkaline phosphatase and C-reactive protein in Hong Kong Chinese
  25. Carbonylation of platelet proteins occurs as consequence of oxidative stress and thrombin activation, and is stimulated by ageing and type 2 diabetes
  26. An automated assay of urinary alanine aminopeptidase activity
  27. Non-high-density lipoprotein cholesterol levels and recent involuntary weight gain among asymptomatic female subjects
  28. Reference Values
  29. Reference intervals for circulating angiogenic cytokines
  30. Hematological parameters in preterm infants from birth to 16 weeks of age with reference to iron balance
  31. Influence of hydroxyethyl starch (6% HES 130/0.4) administration on hematology and clinical chemistry parameters
  32. Letters to the Editor
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  34. Simple human error in data reading and expression in clinical chemistry
  35. Reply to Dr. Park's letter
  36. IFCC Guidelines and Recommendations
  37. Enzymatic assays for creatinine: time for action
  38. Implementation of standardization of HbA1c measurement Summary of the meeting with manufacturers held in Milan, Italy, December 12, 2007
  39. Abstracts
  40. 3rd International Conference on Dipeptidyl Peptidase and Related Proteins Antwerp, Belgium, April 23–25, 2008
  41. Seventh International Symposium on Molecular Diagnostics Graz, Austria, May 22–24, 2008
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