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PROCAM Study: risk prediction for myocardial infarction using microfluidic high-density lipoprotein (HDL) subfractionation is independent of HDL cholesterol
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Odilo Mueller
Published/Copyright:
April 1, 2008
Abstract
Background: High-density lipoprotein (HDL) subfractions are among the new emerging risk factors for atherosclerosis. In particular, HDL 2b has been shown to be linked to cardiovascular risk. This study uses a novel microfluidics-based method to establish HDL 2b clinical utility using samples from the Prospective Cardiovascular Muenster (PROCAM) Study.
Methods: Method performance was established by measuring accuracy, precision, linearity and inter-site precision. Serum samples from 503 individuals collected in the context of the PROCAM study were analyzed by electrophoresis on a microfluidics system. Of these, 251 were male survivors of myocardial infarction (cases), while 252 individuals were matched healthy controls. HDL cholesterol, HDL 2b concentration and HDL 2b percentage were analyzed.
Results: This novel method showed satisfactory assay performance with an inter-site coefficient of variance of <10% for HDL 2b percentage. Parallel patient testing on 52 samples between two sites resulted in a correlation coefficient of r=0.95. Significant differences were observed in the HDL 2b subfraction between cases and controls independent of other risk factors. Including HDL 2b percentage in logistic regression reduced the number of false positives from 64 to 39 and the number of false negative cases from 48 to 45, in the context of this study.
Conclusions: The novel method showed satisfactory assay performance in addition to drastically reduced analysis times and improved ease of use as compared to other methods. Clinical utility of HDL 2b was demonstrated supporting the findings of previous studies.
Clin Chem Lab Med 2008;46:490–8.
Keywords: atherosclerosis; bioanalyzer; HDL 2b; high-density lipoprotein (HDL); myocardial infarction
Received: 2007-9-25
Accepted: 2008-1-8
Published Online: 2008-04-01
Published in Print: 2008-04-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
atherosclerosis;
bioanalyzer;
HDL 2b;
high-density lipoprotein (HDL);
myocardial infarction
Articles in the same Issue
- Editorial
- Improving clinicians understanding of lipoprotein sub-fractions through new technology
- Review
- Free fatty acids as a cardiovascular risk factor
- Opinion Papers
- Pharmacy-based laboratory services: past or future and risk or opportunity?
- Standards of practice and uniformity in references style
- Genetics and Molecular Diagnostics
- Glutathione S-transferase variants increase susceptibility for late-onset Alzheimer's disease: association study and relationship with apolipoprotein E ɛ4 allele
- The D allele of angiotensin I-converting enzyme gene insertion/deletion polymorphism is associated with the severity of atherosclerosis
- The apolipoprotein A5 gene –1131T→C polymorphism affects vitamin E plasma concentrations in type 2 diabetic patients
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- Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?
- Performance characteristics of a real-time RT-PCR assay for quantification of hepatitis C virus RNA in patients with genotype 1 and 2 infections
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- Reply to Dr. Park's letter
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