Nerve growth factor-induced myoprotection in C2C12 muscle cells is mediated by α9β1 integrin via release of PGE2
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Keren Ettinger
Abstract
Background: Nerve growth factor (NGF) mediates a wide range of activities in the central nervous system including neuronal differentiation, synaptic plasticity, and neuroprotection. In addition, NGF places an important role in skeletal muscle physiology by some unknown mechanisms. We recently demonstrated that NGF conferred myoprotection toward ischemia in C2C12 skeletal muscle cell model, establishing an important trophic role for NGF in skeletal muscle.
Methods: In this report, using ELISA and oxygen-glucose deprivation (OGD) assays, we investigated the potential contribution of prostaglandin E2 (PGE2) to NGF myoprotective effects toward C2C12 cultures exposed to OGD insults. Vipera lebetina obtusa disintegrin 5 (VLO5), a selective antagonist of α9β1 integrin, was used as an experimental tool to clarify α9β1 integrin role in NGF action.
Results: NGF-induced mitogen-activated protein kinase type 1 or 2 (ERK1/2) phosphorylation in C2C12 cells and in a dose-response fashion stimulated PGE2 release, both effects antagonized by VLO5 and PD98059. NGF-induced myoprotection of the cells exposed for 7 h to OGD, followed by 18 h of reoxygenation, was reversed by VLO5 treatment.
Conclusions: These results suggest that NGF activation of α9β1 integrin induced myoprotection by stimulation of ERK phosphorylation and release of cytoprotective PGE2 mediator. This effect may be also relevant for NGF-induced pain and hyperalgesia in the skeletal muscle.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Behavior and Neuroprotection
- Evaluation of analgesic activity of levofloxacin in human experimental pain model
- Cardiovascular-Pulmonary Interactions
- Normal spirometric standards in young adult Indian population
- Oxidative Stress
- Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals
- Geraniol attenuates oxidative stress by Nrf2 activation in diet-induced experimental atherosclerosis
- Comparative assessment of onion and garlic extracts on endogenous hepatic and renal antioxidant status in rat
- Hematological Profile
- Silver nanoparticles influenced rat serum metabolites and tissue morphology
- Inflammation
- Anti-inflammatory and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar rats
- Evaluation of anti-inflammatory and analgesic activities and the phytochemical study of Astragalus arbusculinus gum in animal models
- Phytotherapy
- Effects of sub-acute methanol extract treatment of Calliandra portoricensis root bark on antioxidant defence capacity in an experimental rat model
- A hydro-ethanolic extract of Synedrella nodiflora (L.) Gaertn ameliorates hyperalgesia and allodynia in vincristine-induced neuropathic pain in rats
- In vitro assessment of anticholinesterase and NADH oxidase inhibitory activities of an edible fern, Diplazium esculentum
- Original Article
- Identification and evaluation of potentially inappropriate medications (PIMs) in hospitalized geriatric patients using Beers criteria
- Short Communication
- Nerve growth factor-induced myoprotection in C2C12 muscle cells is mediated by α9β1 integrin via release of PGE2
Artikel in diesem Heft
- Frontmatter
- Behavior and Neuroprotection
- Evaluation of analgesic activity of levofloxacin in human experimental pain model
- Cardiovascular-Pulmonary Interactions
- Normal spirometric standards in young adult Indian population
- Oxidative Stress
- Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals
- Geraniol attenuates oxidative stress by Nrf2 activation in diet-induced experimental atherosclerosis
- Comparative assessment of onion and garlic extracts on endogenous hepatic and renal antioxidant status in rat
- Hematological Profile
- Silver nanoparticles influenced rat serum metabolites and tissue morphology
- Inflammation
- Anti-inflammatory and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar rats
- Evaluation of anti-inflammatory and analgesic activities and the phytochemical study of Astragalus arbusculinus gum in animal models
- Phytotherapy
- Effects of sub-acute methanol extract treatment of Calliandra portoricensis root bark on antioxidant defence capacity in an experimental rat model
- A hydro-ethanolic extract of Synedrella nodiflora (L.) Gaertn ameliorates hyperalgesia and allodynia in vincristine-induced neuropathic pain in rats
- In vitro assessment of anticholinesterase and NADH oxidase inhibitory activities of an edible fern, Diplazium esculentum
- Original Article
- Identification and evaluation of potentially inappropriate medications (PIMs) in hospitalized geriatric patients using Beers criteria
- Short Communication
- Nerve growth factor-induced myoprotection in C2C12 muscle cells is mediated by α9β1 integrin via release of PGE2
