Evaluation of analgesic activity of levofloxacin in human experimental pain model
-
Rohita Shetty
,
Sameer Chaudhari
Abstract
Background: Levofloxacin, an optically active isomer of ofloxacin, exerts broad spectrum antibacterial activity. Apart from its antibacterial action, experimental studies have documented antinociceptive properties in animal pain models suggesting an analgesic effect. This study was conducted with the aim to evaluate the analgesic activity of levofloxacin in healthy human volunteers.
Methods: This was an open label, prospective, non-randomized, interventional study conducted on 12 apparently healthy volunteers at a tertiary care teaching hospital in India. Permission from the Institutional Ethics Committee was obtained. After obtaining informed consent, volunteers fulfilling the inclusion criteria were enrolled. Analgesic activity was assessed by Cola cap and sphygmomanometer, a human experimental pain model. Observation of pain threshold and tolerance was recorded at baseline and at an interval of 2 h and 4 h after administration of levofloxacin (500 mg oral) in all 12 subjects. The results were analysed using repeated measures one way analysis of variance.
Results: The mean age of the volunteers was 27.5±3.26 years. A statistically significant increase in the pain threshold and tolerance was found at 2 h and 4 h when compared to baseline.
Conclusions: Levofloxacin exhibited significant analgesic activity in a human pain model and can be used in infections associated with pain or for pre-operative prophylaxis. Further investigations are required to understand the precise mechanism and pathway of antinociception exhibited by levofloxacin.
Acknowledgments
The authors thank the study participants for their support and co-operation.
Author contributions: The authors have all accepted responsibility for the entire content of this submitted manuscript and approved submission.
Financial support: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organisation(s) played no role in the study design, in the collection, analysis, and interpretation of data, in the writing of the report, or in the decision to submit the report for publication.
References
1. Eliopoulos GM, Wennersten CB, Moellering RC. Comparative in vitro activity of levofloxacin and ofloxacin against Gram-positive bacteria. Diagn Microbiol Infect Dis 1996;25:35–41.10.1016/0732-8893(96)00069-7Search in Google Scholar
2. Une T, Fujimoto T, Sato K, Osada Y. In vitro activity of DR-3355, an optically active ofloxacin. Antimicrob Agents Chemother 1988;32:1336–40.10.1128/AAC.32.9.1336Search in Google Scholar
3. Fish DN, Chow AT. The clinical pharmacokinetics of levofloxacin. Clin Pharmacokinet 1997;32:101–19.10.2165/00003088-199732020-00002Search in Google Scholar
4. Suaudeau C, Chait A, Cimetiere C, de Beaurepaire R. Analgesic effects of antibiotics in rats. Pharmacol Biochem Behav 1993;46:361–4.10.1016/0091-3057(93)90364-YSearch in Google Scholar
5. Yildiran G, Ulak G, Utkan T, Göldeli E, Gacar N, Erden F. Antinociception induced by verapamil and chloramphenicol in mice. Biol Neonate 1997;72:28–31.10.1159/000244463Search in Google Scholar
6. Prado WA, Tonussi CR, Rego EM, Corrado AP. Antinociception induced by intraperitoneal injection of gentamicin in rats and mice. Pain 1990;41:365–71.10.1016/0304-3959(90)90013-4Search in Google Scholar
7. Macaluso A, Bernabucci M, Trabucco A, Ciolli L, Troisi F, Baldini R, et al. Analgesic effect of a single preoperative dose of the antibiotic ceftriaxone in humans. J Pain 2013;14:604–12.10.1016/j.jpain.2013.01.774Search in Google Scholar PubMed
8. Luger TJ, Geisler FL, Farkas W, Lorenz IH. Ciprofloxacin increases morphine induced antinociception but not the midazolam-morphine interaction in rats. Eur J Anaesthesiol 2001;18:101.10.1097/00003643-200100001-00358Search in Google Scholar
9. Luger TJ, Farkas W, Geisler H, Lorenz IH. The effect of ciprofloxacin and gentamicin on spinal morphine-induced antinociception in rats. Basic Clin Pharmacol Toxicol 2005;96:366–74.10.1111/j.1742-7843.2005.pto_05.xSearch in Google Scholar PubMed
10. Khobragade AA, Patel SB, Pophale RR, Vallish BN, Kosale SP. Analgesic and anti-inflammatory activity of roxithromycin and erythromycin, alone and in combination with ibuprofen: an animal study. IOSR J Pharmacy 2012;1:15–21.10.9790/3013-01101521Search in Google Scholar
11. Takasuna K, Kasai Y, Usui C, Takahashi M, Hirohashi M, Tamura K, et al. General pharmacology of the new quinolone antibacterial agent levofloxacin. Arzneimittelforschung 1992;43:408–18.Search in Google Scholar
12. Erden BF, Ulak G, Yildiz F, Utkan T, Ozdemirci S, Gacar N. Antidepressant, anxiogenic, and antinociceptive properties of levofloxacin in rats and mice. Pharmacol Biochem Behav 2001;68:435–41.10.1016/S0091-3057(00)00468-8Search in Google Scholar
13. Medhi B, Prakash A, editors. Practical manual of experimental and clinical pharmacology, 1st ed. New Delhi: Jaypee Publications, 2010:322–5.10.5005/jp/books/11381_32Search in Google Scholar
14. Karla J, Chaturvedi A, Karla S, Chaturvedi H, Dhasmana DC. Modulation of pain perception by ramipril and losartan in human volunteers. Indian J Physiol Pharmacol 2008;52:91–6.Search in Google Scholar
15. Akahane K, Kato M, Takayama S. Involvement of inhibitory and excitatory neurotransmitters in levofloxacin- and ciprofloxacin-induced convulsions in mice. Antimicrob Agents Chemother 1993;37:1764–70.10.1128/AAC.37.9.1764Search in Google Scholar PubMed PubMed Central
16. Olesen AE, Andresen T, Staahl C, Drewes AM. Human experimental pain models for assessing the therapeutic efficacy of analgesic drugs. Pharmacol Rev 2012;64:722–79.10.1124/pr.111.005447Search in Google Scholar PubMed
17. Arendt-Nielsen L, Sumikura H. From pain research to pain treatment: role of human pain models. J Nippon Med Sch 2002;69:514–24.10.1272/jnms.69.514Search in Google Scholar PubMed
18. Reddy KS, Naidu MU, Rani PU, Rao TR. Human experimental pain models: A review of standardized methods in drug development. J Res Med Sci 2012;17:587–95.Search in Google Scholar
©2015 by De Gruyter
Articles in the same Issue
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- Behavior and Neuroprotection
- Evaluation of analgesic activity of levofloxacin in human experimental pain model
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Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Evaluation of analgesic activity of levofloxacin in human experimental pain model
- Cardiovascular-Pulmonary Interactions
- Normal spirometric standards in young adult Indian population
- Oxidative Stress
- Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals
- Geraniol attenuates oxidative stress by Nrf2 activation in diet-induced experimental atherosclerosis
- Comparative assessment of onion and garlic extracts on endogenous hepatic and renal antioxidant status in rat
- Hematological Profile
- Silver nanoparticles influenced rat serum metabolites and tissue morphology
- Inflammation
- Anti-inflammatory and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar rats
- Evaluation of anti-inflammatory and analgesic activities and the phytochemical study of Astragalus arbusculinus gum in animal models
- Phytotherapy
- Effects of sub-acute methanol extract treatment of Calliandra portoricensis root bark on antioxidant defence capacity in an experimental rat model
- A hydro-ethanolic extract of Synedrella nodiflora (L.) Gaertn ameliorates hyperalgesia and allodynia in vincristine-induced neuropathic pain in rats
- In vitro assessment of anticholinesterase and NADH oxidase inhibitory activities of an edible fern, Diplazium esculentum
- Original Article
- Identification and evaluation of potentially inappropriate medications (PIMs) in hospitalized geriatric patients using Beers criteria
- Short Communication
- Nerve growth factor-induced myoprotection in C2C12 muscle cells is mediated by α9β1 integrin via release of PGE2
