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Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients
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Eline Verhoye
Veröffentlicht/Copyright:
1. Mai 2009
Abstract
Background: Three adult patients presented with unexpectedly high thyrotropin (TSH) concentrations that were discordant with clinical and biochemical findings of euthyroid or hyperthyroid status.
Methods: Antibody interference in the TSH immunoassay (Roche) was investigated by polyethylene glycol (PEG)-pretreatment, heterophilic blocking tube (HBT)-pretreatment, rheumatoid factor (RF) testing, immunofixation, protein A adsorption, and gel filtration chromatography (GFC).
Results: PEG-precipitation yielded <20% recovery of serum TSH, whereas HBT-pretreatment did not decrease TSH test results. RF-testing and immunofixation were negative. Protein A adsorption and GFC demonstrated the presence of TSH-immunoglobulin complexes in serum.
Conclusions: Interference by TSH-immunoglobulin complexes should be ruled out in euthyroid and hyperthyroid patients presenting with inappropriately increased or non-suppressed TSH values.
Clin Chem Lab Med 2009;47:604–6.
Keywords: antibody interference; electrochemiluminescent immunoassay (ECLIA); polyethylene glycol (PEG); thyroid-stimulating hormone (TSH)
Received: 2009-1-6
Accepted: 2009-3-10
Published Online: 2009-05-01
Published in Print: 2009-05-01
©2009 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
antibody interference;
electrochemiluminescent immunoassay (ECLIA);
polyethylene glycol (PEG);
thyroid-stimulating hormone (TSH)
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- Pharmacogenetics of vitamin K antagonists: useful or hype?
- Genetics and Molecular Diagnostics
- Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells
- Association of interleukin-18 gene promoter polymorphisms with risk of acute myocardial infarction in northern Chinese Han population
- MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
- Predictive value of CA 125 and CA 72-4 in ovarian borderline tumors
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- General Clinical Chemistry and Laboratory Medicine
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