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MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis

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Published/Copyright: March 23, 2009
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 47 Issue 5

Abstract

Background: Detection of tumor-associated genetic alterations in plasma of cancer patients has recently been suggested to be an accurate method for detecting early or recurrent cancer.

Methods: We performed quantitative real-time PCR for MYC and GAPDH in tissue and plasma samples of 57 patients with gastric cancer and in plasma of 79 cancer-free individuals. We also performed two-color MYC fluorescence in situ hybridization (FISH) in tissue from the 57 patients with gastric cancer.

Results: The tissue MYC/GAPDH ratio by real-time PCR was significantly correlated with MYC status by FISH (p<0.001). The mean ratio of plasma MYC/GAPDH was 5.226±3.578 (range: 1.25–18.35) in gastric cancer patients, and 2.436±0.881 (range: 1.00–5.00) in the healthy volunteers (p<0.001). We used receiver-operating characteristics (ROC) curve analysis to select two optimal plasma MYC/GAPDH cut-offs of 2.725 and 5.225. The sensitivity and specificity were 75.4% and 76.9% at 2.725, 38.6% and 100% at 5.225, respectively. The plasma MYC/GAPDH ratio from cancer patients was significantly correlated with the tissue MYC/GAPDH ratio (p=0.009), and tissue MYC status by FISH (p=0.024).

Conclusions: These findings suggest that the plasma MYC/GAPDH ratio, as determined by real-time PCR, may be an alternative non-invasive approach for detecting gastric cancer.

Clin Chem Lab Med 2009;47:530–6.

Keywords: MYC; plasma DNA; real-time PCR; stomach cancer
Corresponding author: Hye Seung Lee, MD, Department of Pathology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-707, Korea Phone: +82-31-787-7714, Fax: +82-31-787-4012,
Received: 2008-12-25
Accepted: 2009-2-9
Published Online: 2009-03-23
Published in Print: 2009-05-01

©2009 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2009.126
Keywords for this article
MYC; plasma DNA; real-time PCR; stomach cancer

Articles in the same Issue

  1. Review
  2. Pharmacogenetics of vitamin K antagonists: useful or hype?
  3. Genetics and Molecular Diagnostics
  4. Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells
  5. Association of interleukin-18 gene promoter polymorphisms with risk of acute myocardial infarction in northern Chinese Han population
  6. MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
  7. Predictive value of CA 125 and CA 72-4 in ovarian borderline tumors
  8. Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells
  9. General Clinical Chemistry and Laboratory Medicine
  10. Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
  11. Determination of urinary stone composition based on stone morphology: a prospective study of 325 consecutive patients in an emerging country
  12. Determination of branched chain amino acids, methionine, phenylalanine, tyrosine and α-keto acids in plasma and dried blood samples using HPLC with fluorescence detection
  13. Hematocytometry analysis as discriminative marker for asthma phenotypes
  14. A routine method for the quantification of the novel antimycotic drug posaconazole in plasma using liquid chromatography-tandem mass spectrometry
  15. Reference Values and Biological Variations
  16. Serum β2-microglobulin measured by immunonephelometry: expression patterns and reference intervals in healthy adults
  17. Serum enzyme profile characteristics of victims following the Wenchuan earthquake in China
  18. Stability of selected serum proteins after long-term storage in the Janus Serum Bank
  19. Spuriously high thyrotropin values due to anti-thyrotropin antibodies in adult patients
  20. Validation and Outcome Studies
  21. Workflow restrictions on hematology analyzers XE-2100 and XS-800 when assaying pediatric samples with limited volume
  22. Letters to the Editor
  23. Carbamylated hemoglobin remains a critical issue in HbA1c measurements
  24. Detection of a monoclonal gammopathy by serum index measurement
  25. Survey on the prevalence of hemolytic specimens in an academic hospital according to collection facility: opportunities for quality improvement
  26. Quantitative scanning analysis of a cryoglobulin ring detected in sera of patients with hepatitis C using a cooling gel diffusion method
  27. Significant reduction of erythrocyte glucose-6-phosphate dehydrogenase activity in soccer-players during play. Evidence for catecholamine mediated enzyme inhibition
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