Hematocytometry analysis as discriminative marker for asthma phenotypes
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Karin J. Velthove
Abstract
Background: There is an increasing demand for easy to measure biomarkers in clinical practice. We created the relational database Utrecht Patient Oriented Database (UPOD) to develop tools for identifying new biomarkers for disease. In this study, we used UPOD to identify better biomarkers for discriminating different asthma phenotypes.
Methods: We performed a prospective study at the University Medical Center (UMC) Utrecht using blood from patients with asthma and a healthy reference group. Since asthma is an inflammatory disease, absolute leukocyte counts and leukocyte differential parameters were analyzed using raw data files and a logistic regression model.
Results: We compared 17 difficult-to-treat asthma (DTA) cases, 13 non-difficult-to-treat asthma cases, and 19 healthy volunteers. Absolute leukocyte counts and differential parameters for leukocytes were able to discriminate asthma patients from healthy volunteers. However, among patients with asthma, difficult-to-treat cases could be more accurately defined with a neutrophil morphology change (OR 8.0; 95% CI 1.5–42.0), compared to the absolute neutrophil count (OR 4.0; 95% CI 0.8–21.0).
Conclusions: In this asthma patient population, we were able to define asthma phenotypes more precisely using neutrophil morphology parameters, compared to absolute counts. Using UPOD with differential parameters, it is possible to conduct larger scale biomarker studies, combining clinical, laboratory medicine, and epidemiological techniques.
Clin Chem Lab Med 2009;47:573–8.
©2009 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Review
- Pharmacogenetics of vitamin K antagonists: useful or hype?
- Genetics and Molecular Diagnostics
- Illumina DNA test panel-based genotyping of whole genome amplified-DNA extracted from hair samples: performance and agreement with genotyping results from genomic DNA from buccal cells
- Association of interleukin-18 gene promoter polymorphisms with risk of acute myocardial infarction in northern Chinese Han population
- MYC quantitation in cell-free plasma DNA by real-time PCR for gastric cancer diagnosis
- Predictive value of CA 125 and CA 72-4 in ovarian borderline tumors
- Anti DNA antibodies are not restricted to a specific pattern of fluorescence on HEp2 cells
- General Clinical Chemistry and Laboratory Medicine
- Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry
- Determination of urinary stone composition based on stone morphology: a prospective study of 325 consecutive patients in an emerging country
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