Study on the Interaction Between Cellulase and Surfactants
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Jian Zhang
Abstract
The conformations of secondary and tertiary structures of cellulase in the presence of eleven commonly used surfactants were determined by Raman spectroscopy and the results were discussed. The results indicated that anionic surfactants had a stronger influence on the cellulase conformations than nonionic surfactants. Thus anionic surfactants showed a stronger inactivation on the cellulase activity. Furthermore, Zeta potential distributions of cellulase in solutions of surfactants were tested by Dynamic Light Scattering (DLS). The DLS results indicated that the interaction between anionic surfactants and cellulase was attributed to electrostatic attraction. By adding cellulase to a liquid, non-cellulase-containing detergent, the detergency of the liquid detergent could be increased. Further studies on the sample swatches by optical microscopy and scanning electron microscopy (SEM) were undertaken in this paper.
Kurzfassung
Die Konformationen der sekundären und der tertiären Strukturen von Cellulase in Gegenwart von elf häufig verwendeten Tensiden wurden mit Hilfe der Ramanspektroskopie bestimmt und die Ergebnisse diskutiert. Die Ergebnisse zeigten, dass anionische Tenside einen stärkeren Einfluss auf die Cellulase-Konformationen hatten als nichtionische Tenside. Anionische Tenside inaktivierten Cellulase stärker. Darüber hinaus wurden Zetapotentialverteilungen von Cellulase in Tensid-Lösungen mittels dynamischer Lichtstreuung (DLS) bestimmt. Die DLS-Ergebnisse zeigten, dass die Wechselwirkung zwischen anionischen Tensiden und Cellulase von der elektrostatischen Anziehung bestimmt wurde. Durch Hinzugeben von Cellulase in ein flüssiges, nicht cellulasehaltiges Waschmittel konnte die Waschleistung des Waschmittels erhöht werden. Weitere Untersuchungen der Testgewebe mittels optischer Mikroskopie und Rasterelektronenmikroskopie (SEM) wurden in dieser Arbeit durchgeführt.
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© 2017, Carl Hanser Publisher, Munich
Artikel in diesem Heft
- Contents/Inhalt
- Contents
- Detergent/Enzymes
- Lactobacillus brevis Lipase: Purification, Immobilization onto Magnetic Florosil NPs, Characterization and Application as a Detergent Additive
- Study on the Interaction Between Cellulase and Surfactants
- Physical Chemistry
- Study of Ionic Liquid Microemulsions: Ethylammonium Nitrate/TritonX-100/Cyclohexane
- Synergistic Effect of Cationic Surfactants on the Rheological Behavior of Erucyl Amidosulfobetaine
- Microscopic Evidence for the Correlation of Micellar Structures and Counterion Binding Constant for Flexible Nanoparticle Catalyzed Piperidinolysis of PS− in Colloidal System
- Application
- Effect of Surface Modification on the Dispersion, Thermal Stability and Crystallization Properties of PET/CaCO3 Nanocomposites
- Environmental Chemistry
- Inhibition of Calcium Carbonate Scale Using an Environmental Friendly Scale Inhibitor
- Novel Surfactants
- Study on the Properties of Mixed Micelles of Disodium Salt of 3-({2-[(2-Carboxy-ethyl)-dodecanoyl-amino]-ethyl}-dodecanoyl-amino)-propionic Acid in Solution Systems
- Synthesis
- Macrocyclic Schiff Base Metal Complexes Derived from Isatin: Structural Activity Relationship and DFT Calculations
- Quaternary Ammonium Gemini Surfactants Used in Enhanced Oil Recovery: Synthesis, Properties, and Flooding Experiments
Artikel in diesem Heft
- Contents/Inhalt
- Contents
- Detergent/Enzymes
- Lactobacillus brevis Lipase: Purification, Immobilization onto Magnetic Florosil NPs, Characterization and Application as a Detergent Additive
- Study on the Interaction Between Cellulase and Surfactants
- Physical Chemistry
- Study of Ionic Liquid Microemulsions: Ethylammonium Nitrate/TritonX-100/Cyclohexane
- Synergistic Effect of Cationic Surfactants on the Rheological Behavior of Erucyl Amidosulfobetaine
- Microscopic Evidence for the Correlation of Micellar Structures and Counterion Binding Constant for Flexible Nanoparticle Catalyzed Piperidinolysis of PS− in Colloidal System
- Application
- Effect of Surface Modification on the Dispersion, Thermal Stability and Crystallization Properties of PET/CaCO3 Nanocomposites
- Environmental Chemistry
- Inhibition of Calcium Carbonate Scale Using an Environmental Friendly Scale Inhibitor
- Novel Surfactants
- Study on the Properties of Mixed Micelles of Disodium Salt of 3-({2-[(2-Carboxy-ethyl)-dodecanoyl-amino]-ethyl}-dodecanoyl-amino)-propionic Acid in Solution Systems
- Synthesis
- Macrocyclic Schiff Base Metal Complexes Derived from Isatin: Structural Activity Relationship and DFT Calculations
- Quaternary Ammonium Gemini Surfactants Used in Enhanced Oil Recovery: Synthesis, Properties, and Flooding Experiments
