Bench-scale biosynthesis of isonicotinic acid from 4-cyanopyridine by Pseudomonas putida
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Xiao-Yan Zhu
,
Jin-Song Gong
Abstract
Pseudomonas putida CGMCC3830 harboring nitrilase was used in isonicotinic acid production from 4-cyanopyridine. This nitrilase showed optimum activities towards 4-cyanopyridine at pH 7.5 and 45°C. The half-life of P. putida nitrilase was 93.3 h, 33.9 h, and 9.5 h at 30°C, 38°C, and 45°C, respectively. 4-Cyanopyridine (100 mM) was fully converted into isonicotinic acid within 20 min. The bench-scale production of isonicotinic acid was carried out using 3 mg of resting cells per mL in a 1 L system at 30°C and finally, 123 g L−1 of isonicotinic acid were obtained within 200 min without any by-products. The conversion reaction suffered from the product inhibition effect after the tenth feeding. The volumetric productivity was 36.9 g L−1 h−1. P. putida shows significant potential in nitrile hydrolysis for isonicotinic acid production. This paper is the first report on isonicotinic acid biosynthesis using Pseudomonas putida and it represents the highest isonicotinic acid production reported so far.
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© 2013 Institute of Chemistry, Slovak Academy of Sciences
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Articles in the same Issue
- Rapid determination of fosetyl-aluminium in commercial pesticide formulations by high-performance liquid chromatography
- Immobilisation of acid pectinase on graphene oxide nanosheets
- Bench-scale biosynthesis of isonicotinic acid from 4-cyanopyridine by Pseudomonas putida
- Enzymatic synthesis of a chiral chalcogran intermediate
- Separation of Cd(II) and Ni(II) ions by supported liquid membrane using D2EHPA/M2EHPA as mobile carrier
- Fouling of nanofiltration membranes used for separation of fermented glycerol solutions
- Oxyhumolite influence on adsorption and desorption of phosphate on blast furnace slag in the process of two-stage selective adsorption of Cu(II) and phosphate
- Cellulose-precipitated calcium carbonate composites and their effect on paper properties
- Landfill leachate treatment using the sequencing batch biofilm reactor method integrated with the electro-Fenton process
- Effect of sintering temperature on the magnetic properties and charge density distribution of nano-NiO
- Synthesis, optimization, characterization, and potential agricultural application of polymer hydrogel composites based on cotton microfiber
- Cu(II) removal enhancement from aqueous solutions using ion-imprinted membrane technique
- Synthesis of new eburnamine-type alkaloid via direct hydroalkoxylation
- Selection of surfactants as main components of ecological wetting agent for effective extinguishing of forest and peat-bog fires
- Ultrasonic and Lewis acid ionic liquid catalytic system for Kabachnik-Fields reaction
- A simple method for creating molecularly imprinted polymer-coated bacterial cellulose nanofibers
- Determination of pK a of N-alkyl-N,N-dimethylamine-N-oxides using 1H NMR and 13C NMR spectroscopy
